POLYOL OXIDASE
    1.
    发明公开
    POLYOL OXIDASE 有权
    多元醇氧化酶

    公开(公告)号:EP2772537A1

    公开(公告)日:2014-09-03

    申请号:EP12843544.3

    申请日:2012-10-26

    IPC分类号: C12N9/04 C12P19/02 C12N15/09

    CPC分类号: C12N9/0006 C12P19/02 C12R1/80

    摘要: Problem: To provide a novel polyol oxidase. Solution: A polyol oxidase, which is derived from a microorganism belonging to the genus Penicillium and is specified by the properties listed in the following (a) to (e): (a) it has a stable pH of 6.0 or higher and an optimum reaction pH of from 7.0 to 9.0; (b) it has an operating temperature of 50°C or lower and an optimum reaction temperature of 40°C; (c) it has a molecular weight of about 113 kDa; (d) it specifically recognizes the structure of a polyol in which the OH groups at positions 2 and 3 are in the L-erythro configuration and reacts with the polyol, but cannot recognize the structure of a polyol in which the OH group at position 4 is in the L-ribo configuration and does not react with the polyol; and (e) it has substrate specificity for D-arabitol, erythritol, D-mannitol, and D-sorbitol, which are listed in descending order of specificity.

    摘要翻译: 问题:提供一种新型的多元醇氧化酶。 解决方案:一种多元醇氧化酶,来自属于青霉属的微生物,由下列(a)至(e)中列出的特性指定:(a)它具有6.0或更高的稳定pH值和最佳 反应pH值为7.0至9.0; (b)工作温度为50°C或更低,最佳反应温度为40°C; (c)它具有约113kDa的分子量; (d)具体地确认了2位和3位的OH基为L-赤型构型并与多元醇反应的多元醇的结构,但是不能识别其中位置4处的OH基团 处于L-核糖构型并且不与多元醇反应; 和(e)它对D-阿拉伯糖醇,赤藓糖醇,D-甘露糖醇和D-山梨醇具有底物特异性,按特异性的降序列出。

    KETOSE 3-EPIMERASE PRODUCED BY ARTHROBACTER GLOBIFORMIS
    6.
    发明公开
    KETOSE 3-EPIMERASE PRODUCED BY ARTHROBACTER GLOBIFORMIS 审中-公开
    DURCH ARTHROBACTER GLOBIFORMIS HERGESTELLTE KETOSE-3-EPIMERASE

    公开(公告)号:EP2944691A1

    公开(公告)日:2015-11-18

    申请号:EP13870446.5

    申请日:2013-12-26

    IPC分类号: C12N9/90 C12N15/09 C12P19/02

    摘要: There are provided a highly safe epimerase usable in food industry, and a method for producing a ketose. The epimerase is a ketose 3-epimerase obtainable from a microorganism of the genus Arthrobacter , and having the amino acid sequence represented by SEQ ID NO: 1 of the Sequence Listing, and (1) substrate specificity whereby a D- or L-ketose is epimerized at position 3 to produce a corresponding D- or L-ketose, and (2) the highest substrate specificity for D-fructose and D-psicose among D- and L-ketoses. The ketose 3-epimerase is also represented by SEQ ID NO: 3 or SEQ ID NO: 4 of the Sequence Listing, and epimerizes a D- or L-ketose at position 3 to produce a corresponding D- or L-ketose.

    摘要翻译: 提供了可用于食品工业的高度安全的差向异构酶,以及生产酮糖的方法。 差向异构酶是从节杆菌属的微生物获得并具有由序列表的SEQ ID NO:1表示的氨基酸序列的酮糖3-差向异构酶,和(1)底物特异性,其中D-或L-酮糖是 在位置3差向异构化以产生相应的D-或L-酮糖,和(2)D和L-酮糖之中D-果糖和D-灵芝蛋白的最高底物特异性。 酮基3-差向异构酶也由序列表的SEQ ID NO:3或SEQ ID NO:4表示,并且在3位差异化D-或L-酮糖以产生相应的D-或L-酮糖。

    ENZYME PRODUCED BY ARTHROBACTER GLOBIFORMIS
    8.
    发明公开
    ENZYME PRODUCED BY ARTHROBACTER GLOBIFORMIS 有权
    AUS ARTHROBACTER-GLOBIFORMIEN HERGESTELLTES ENZYM

    公开(公告)号:EP2730652A1

    公开(公告)日:2014-05-14

    申请号:EP12807548.8

    申请日:2012-07-05

    IPC分类号: C12N9/90 C12P19/02

    CPC分类号: C12N9/90 C12P19/02 C12P19/24

    摘要: Task: There are provided a highly safe epimerase usable in food industry, and a method for producing a ketose. Resolution: The epimerase is a ketose 3-epimerase obtainable from a microorganism of the genus Arthrobacter, and having (1) substrate specificity whereby a D- or L-ketose is epimerized at position 3 to produce a corresponding D- or L-ketose, and (2) the highest substrate specificity for D-fructose and D-psicose among D- and L-ketoses.

    摘要翻译: 任务:提供了可用于食品工业的高度安全的差向异构酶,以及生产酮糖的方法。 分辨率:差向异构酶是可从节杆菌属微生物获得的酮糖3-差向异构酶,并具有(1)底物特异性,其中D-或L-酮糖在3位差向异构化以产生相应的D-或L-酮糖, 和(2)D和L-酮糖中D-果糖和D-灵芝蛋白的底物特异性最高。