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1.发明公开HANSENULA POLYMORPHA YAPSIN DEFICIENT MUTANT STRAIN AND PROCESS FOR THE PREPARATION OF RECOMBINANT PROTEINS USING THE SAME 有权多形汉逊和方法的YAPSIN亏突变株FOR SO生产重组蛋白的
公开(公告)号：EP1534835A1
公开(公告)日：2005-06-01
申请号：EP03736354.6
申请日：2003-06-28
申请人： Korea Research Institute of Bioscience and Biotechnology , Leadbio Inc. , Bioholdings Co., Ltd.
发明人： KANG, Hyun-Ah , RHEE, Sang-Ki , SOHN, Min-Jeong , KIM, Jeong-Yoon
IPC分类号： C12N15/31
CPC分类号： C12N9/58 , C07K2319/02 , C12N15/815 , C12P21/02 , C12P21/06
摘要： The present invention relates to polynucleotide containing HpYPS1 gene coding Hansenula polymorpha yapsin1; polypeptide coded thereby; Hansenula polymorpha mutant wherein yapsin activity is reduced by the mutation of the HpYPS1 gene coding Hansenula polymorpha yapsin1; recombinant Hansenula polymorpha strain expressing a foreign protein prepared by introducing a gene coding a foreign protein to the Hansenula polymorpha mutant; and a method for preparing a foreign protein comprising the steps of culturing the recombinant Hansenula polymorpha strain under the condition that the foreign protein can be expressed, and isolating the foreign protein from the obtained culture broth.
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2.发明授权HANSENULA POLYMORPHA YAPSIN DEFICIENT MUTANT STRAIN AND PROCESS FOR THE PREPARATION OF RECOMBINANT PROTEINS USING THE SAME 有权多形汉逊和方法的YAPSIN亏突变株FOR SO生产重组蛋白的
公开(公告)号：EP1534835B1
公开(公告)日：2010-09-08
申请号：EP03736354.6
申请日：2003-06-28
申请人： Korea Research Institute of Bioscience and Biotechnology , Leadbio Inc. , Bioholdings Co., Ltd.
发明人： KANG, Hyun-Ah , RHEE, Sang-Ki , SOHN, Min-Jeong , KIM, Jeong-Yoon
IPC分类号： C12N15/31
CPC分类号： C12N9/58 , C07K2319/02 , C12N15/815 , C12P21/02 , C12P21/06
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3.发明公开HANSENULA POLYMORPHA YAPSIN DEFICIENT MUTANT STRAIN AND PROCESS FOR THE PREPARATION OF RECOMBINANT PROTEINS USING THE SAME 有权YAPSIN-MANGELMUTANTENSTAMM VON HANSENULA POLYMORPHA SOWIE VERFAHREN ZUR HERSTELLUNG REKOMBINANTER PROTEINE DAMIT
公开(公告)号：EP1534835A4
公开(公告)日：2005-12-14
申请号：EP03736354
申请日：2003-06-28
发明人： KANG HYUN-AH , RHEE SANG-KI , SOHN MIN-JEONG , KIM JEONG-YOON
CPC分类号： C12N9/58 , C07K2319/02 , C12N15/815 , C12P21/02 , C12P21/06
摘要： The present invention relates to polynucleotide containing HpYPS1 gene coding Hansenula polymorpha yapsin1; polypeptide coded thereby; Hansenula polymorpha mutant wherein yapsin activity is reduced by the mutation of the HpYPS1 gene coding Hansenula polymorpha yapsin1; recombinant Hansenula polymorpha strain expressing a foreign protein prepared by introducing a gene coding a foreign protein to the Hansenula polymorpha mutant; and a method for preparing a foreign protein comprising the steps of culturing the recombinant Hansenula polymorpha strain under the condition that the foreign protein can be expressed, and isolating the foreign protein from the obtained culture broth.
摘要翻译： 本发明涉及含有编码多形汉逊酵母Yapsin1的HpYPS1基因的多核苷酸; 由此编码的多肽; 多形汉逊酵母突变体，其中由于编码多形汉逊酵母Yapsin1的HpYPS1基因的突变降低了耶那囊蛋白活性; 表达外源蛋白质的重组多形汉逊酵母菌株，其通过将编码外源蛋白质的基因导入多形汉逊酵母突变体而制备; 以及制备外源蛋白质的方法，该方法包括在外源蛋白质可以表达的条件下培养重组多形汉逊酵母菌株并从所得培养液中分离外源蛋白质的步骤。
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