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公开(公告)号：EP4230746A3
公开(公告)日：2023-11-01
申请号：EP23168994.4
申请日：2018-05-25
申请人： 10X Genomics, Inc.
IPC分类号： C12Q1/68 , C12Q1/6806
摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets or wells) including a single biological particle (e.g., a cell) and a single solid or semi-solid particle (e.g., bead) comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.
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公开(公告)号：EP3700672A1
公开(公告)日：2020-09-02
申请号：EP18801153.0
申请日：2018-10-24
申请人： 10X Genomics, Inc.
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公开(公告)号：EP4375376A3
公开(公告)日：2024-08-14
申请号：EP24167618.8
申请日：2018-04-26
申请人： 10X Genomics, Inc.
发明人： LEE, Josephine , MARRS, Samuel , MCDERMOTT, Geoffrey , MESCHI, Francesca , MONTESCLAROS, Luz , PFEIFFER, Katherine , SHUGA, Joseph Francis , TERRY, Jessica Michele , ZIRALDO, Solongo B.
IPC分类号： C12N9/12 , C12N9/22 , C12N15/33 , C12Q1/6806 , C12Q1/6869
CPC分类号： C12N9/1276 , C12Y207/07049 , C07K2319/2120130101 , C07K2319/5020130101 , C12Q1/6869 , C12Q1/6806
摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.
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公开(公告)号：EP4241882A3
公开(公告)日：2023-12-06
申请号：EP22216643.1
申请日：2018-10-24
申请人： 10X Genomics, Inc.
发明人： BELHOCINE, Kamila , LEE, Josephine , MESCHI, Francesca , MONTESCLAROS, Luz , PFEIFFER, Katherine , PRICE, Andrew D. , SAPIDA, Jerald , SUKOVICH, David , WHEELER, Tobias Daniel , YIN, Yifeng
摘要： The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.
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公开(公告)号：EP4107282A1
公开(公告)日：2022-12-28
申请号：EP21712302.5
申请日：2021-02-19
申请人： 10X Genomics, Inc.
发明人： KOHLWAY, Andrew Scott , MESCHI, Francesca , CHEW, Jennifer , ARTHUR, Joseph Glenn , DELANEY, Nigel , BENT, Zachary , PFEIFFER, Katherine , HILL, Andrew John , ALVARADO MARTINEZ, Luigi Jhon
IPC分类号： C12Q1/6806 , C12Q1/6834
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公开(公告)号：EP4375376A2
公开(公告)日：2024-05-29
申请号：EP24167618.8
申请日：2018-04-26
申请人： 10X Genomics, Inc.
发明人： LEE, Josephine , MARRS, Samuel , MCDERMOTT, Geoffrey , MESCHI, Francesca , MONTESCLAROS, Luz , PFEIFFER, Katherine , SHUGA, Joseph Francis , TERRY, Jessica Michele , ZIRALDO, Solongo B.
IPC分类号： C12N15/33
CPC分类号： C12N9/1276 , C12Y207/07049 , C07K2319/2120130101 , C07K2319/5020130101
摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.
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公开(公告)号：EP4230746A2
公开(公告)日：2023-08-23
申请号：EP23168994.4
申请日：2018-05-25
申请人： 10X Genomics, Inc.
IPC分类号： C12Q1/68 , C12Q1/6806
摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets or wells) including a single biological particle (e.g., a cell) and a single solid or semi-solid particle (e.g., bead) comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.
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公开(公告)号：EP4241882A2
公开(公告)日：2023-09-13
申请号：EP22216643.1
申请日：2018-10-24
申请人： 10X Genomics, Inc.
发明人： BELHOCINE, Kamila , LEE, Josephine , MESCHI, Francesca , MONTESCLAROS, Luz , PFEIFFER, Katherine , PRICE, Andrew D. , SAPIDA, Jerald , SUKOVICH, David , WHEELER, Tobias Daniel , YIN, Yifeng
摘要： The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.
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公开(公告)号：EP3830289A1
公开(公告)日：2021-06-09
申请号：EP19755475.1
申请日：2019-08-02
申请人： 10X Genomics, Inc.
发明人： BELL, Jason , LEE, Josephine , NEMEC, Corey , MESCHI, Francesca
IPC分类号： C12Q1/6806
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公开(公告)号：EP3592847A1
公开(公告)日：2020-01-15
申请号：EP18791768.7
申请日：2018-04-26
申请人： 10X Genomics, Inc.
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