公开(公告)号：EP4375376A3

公开(公告)日：2024-08-14

申请号：EP24167618.8

申请日：2018-04-26

申请人： 10X Genomics, Inc.

发明人： LEE, Josephine ,  MARRS, Samuel ,  MCDERMOTT, Geoffrey ,  MESCHI, Francesca ,  MONTESCLAROS, Luz ,  PFEIFFER, Katherine ,  SHUGA, Joseph Francis ,  TERRY, Jessica Michele ,  ZIRALDO, Solongo B.

IPC分类号： C12N9/12 ,  C12N9/22 ,  C12N15/33 ,  C12Q1/6806 ,  C12Q1/6869

CPC分类号： C12N9/1276 ,  C12Y207/07049 ,  C07K2319/2120130101 ,  C07K2319/5020130101 ,  C12Q1/6869 ,  C12Q1/6806

摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.

公开(公告)号：EP4225934A1

公开(公告)日：2023-08-16

申请号：EP21802873.6

申请日：2021-10-08

申请人： 10X Genomics, Inc.

发明人： MCDONNELL, Wyatt, James ,  STUBBINGTON, Michael, John, Terry ,  MCDERMOTT, Geoffrey

IPC分类号： C12Q1/6804 ,  C12Q1/6869

公开(公告)号：EP4194855A1

公开(公告)日：2023-06-14

申请号：EP22209155.5

申请日：2020-10-09

申请人： 10X Genomics, Inc.

发明人： MCDERMOTT, Geoffrey ,  TAYLOR, Sarah ,  BOUTET, Stephane Claude ,  MCDONNELL, Wyatt James ,  STUBBINGTON, Michael John Terry

IPC分类号： G01N33/543 ,  C12Q1/6806

摘要： Provided herein are methods and systems for measuring secreted cytokines or other analytes from single cells. The methods disclosed herein include the use of analyte-specific and/or barcoded binding agents (e.g., antibodies) and beads in partitions (e.g., droplets or wells) to measure such analytes on a single cell basis. Further described herein are methods comprising the use of hydrogel-encapsulated cells (e.g., cell beads) to measure secreted and/or cellular analytes from single cells.

公开(公告)号：EP4025709A1

公开(公告)日：2022-07-13

申请号：EP20775530.7

申请日：2020-09-04

申请人： 10X Genomics, Inc.

发明人： LANCE, Shea T. ,  MCDERMOTT, Geoffrey ,  WYATT, Paul William ,  RIORDAN, Daniel P. ,  SCHNALL-LEVIN, Michael

IPC分类号： C12Q1/6806

公开(公告)号：EP3896171A1

公开(公告)日：2021-10-20

申请号：EP21169849.3

申请日：2017-12-22

申请人： 10X Genomics, Inc.

发明人： BELGRADER, Phillip ,  BENT, Zachary ,  GOPALAN, Vijay Kumar Sreenivasa ,  HARADA, Josephine ,  HINDSON, Christopher ,  LENJI, Mohammad Rahimi ,  MCDERMOTT, Geoffrey ,  MEER, Elliott ,  MIKKELSEN, Tarjei Sigurd ,  O'KEEFFE, Christopher Joachim ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RYVKIN, Paul ,  SAXONOV, Serge ,  STUELPNAGEL, John R. ,  TERRY, Jessica Michele

IPC分类号： C12Q1/6804 ,  C12N15/10 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for tagging analytes for characterization.

公开(公告)号：EP3894585A2

公开(公告)日：2021-10-20

申请号：EP19835340.1

申请日：2019-12-06

申请人： 10X Genomics, Inc.

发明人： SCHNALL-LEVIN, Michael ,  RAMACHANDRAN IYER, Eswar Prasad ,  YIN, Yifeng ,  SHAH, Preyas ,  MCDERMOTT, Geoffrey ,  LOWE, Adam ,  DELANEY, Joshua ,  TENTORI, Augusto Manuel ,  BHARADWAJ, Rajiv ,  GIACOMELLO, Stefania ,  GAGNON, Alex ,  FREY, Meghan L. F. ,  BENT, Zachary ,  BORGSTROM, Erik Leonard Henrik ,  MIKKELSEN, Tarjei Sigurd

IPC分类号： C12Q1/6837 ,  C12Q1/6841

公开(公告)号：EP3775271A1

公开(公告)日：2021-02-17

申请号：EP19721887.8

申请日：2019-04-01

申请人： 10X Genomics, Inc.

发明人： BENT, Zachary ,  RYVKIN, Paul ,  TERRY, Jessica Michele ,  MCDERMOTT, Geoffrey ,  FREY, Meghan

IPC分类号： C12Q1/6806 ,  C12Q1/6869

公开(公告)号：EP4271509A1

公开(公告)日：2023-11-08

申请号：EP21848455.8

申请日：2021-12-29

申请人： 10X Genomics, Inc.

发明人： MCDERMOTT, Geoffrey ,  PATTERSON, David Michael ,  PRICE, Andrew D. ,  RAMACHANDRAN IYER, Eswar Prasad ,  SCHNALL-LEVIN, Michael

IPC分类号： B01J19/00 ,  C12Q1/6834

公开(公告)号：EP4230746A3

公开(公告)日：2023-11-01

申请号：EP23168994.4

申请日：2018-05-25

申请人： 10X Genomics, Inc.

发明人： BELHOCINE, Zahra, Kamila ,  MCDERMOTT, Geoffrey ,  MESCHI, Francesca ,  ZHENG, Xinying

IPC分类号： C12Q1/68 ,  C12Q1/6806

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets or wells) including a single biological particle (e.g., a cell) and a single solid or semi-solid particle (e.g., bead) comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：EP4022309A1

公开(公告)日：2022-07-06

申请号：EP20800449.9

申请日：2020-10-09

申请人： 10X Genomics, Inc.

发明人： MCDERMOTT, Geoffrey ,  TAYLOR, Sarah ,  BOUTET, Stephane Claude ,  MCDONNELL, Wyatt James ,  STUBBINGTON, Michael John Terry

IPC分类号： G01N33/543 ,  C12Q1/6806