公开(公告)号：EP4428229A1

公开(公告)日：2024-09-11

申请号：EP22889998.5

申请日：2022-11-02

申请人： AGC INC. ,  Kyoto Prefectural Public University Corporation ,  Shinshu University

发明人： KISHIMOTO, Megumi ,  SHIMIZU, Yuto ,  KUBOTA, Susumu ,  YAGYU, Shigeki ,  SUEMATSU, Masaya ,  NAKAZAWA, Yozo ,  TANAKA, Miyuki

IPC分类号： C12N5/0783 ,  C12N15/11

CPC分类号： C12N15/11 ,  C12N5/06

摘要： The present invention provides a method for producing a chimeric antigen receptor T (CAR-T) cell, including a step of bead separating with a specific factor a T cell from a T cell source, wherein the specific factor is CD45RA+ or the like. The method enables production of CAR-T cells high antitumor property, and can produce highly functional CAR-T cells conveniently in a short time at a low cost.

公开(公告)号：EP4006148A1

公开(公告)日：2022-06-01

申请号：EP20847793.5

申请日：2020-07-30

申请人： Shinshu University ,  Kyoto Prefectural Public University Corporation ,  BrightPath Biotherapeutics Co., Ltd.

发明人： NAKAZAWA, Yozo ,  YAGYU, Shigeki ,  TANAKA, Miyuki ,  NAKAMURA, Kayoko ,  OKADA, Masahiro ,  KONDO, Makoto ,  SHIGEURA, Tomokuni ,  HIROTA, Shogo

IPC分类号： C12N5/10 ,  C12N15/12 ,  C12N15/62 ,  C12N15/85 ,  A61K35/17

摘要： The present disclosure includes a method of producing a cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells, comprising co-culturing CAR-expressing immune cells and cells expressing a target antigen of the CAR, wherein the CAR-expressing immune cells are cells into which a CAR gene has been introduced and the target antigen-expressing cells are normal blood cells that have been engineered to express the target antigen.

公开(公告)号：EP3872087A1

公开(公告)日：2021-09-01

申请号：EP19877316.0

申请日：2019-10-25

申请人： Shinshu University

发明人： NAKAZAWA, Yozo ,  TANAKA, Miyuki ,  MOROKAWA, Hirokazu ,  NARIMATSU, Shogo

IPC分类号： C07K14/705 ,  C12N5/0783 ,  C12N5/10 ,  C12N15/12 ,  C12N15/63

摘要： An object of the present invention is to improve the efficiency of a method for producing chimeric antigen receptor (CAR)-expressing cells. The present invention provides a method for producing genetically modified mammalian cells, comprising the steps of: a) introducing a polynucleotide encoding a chimeric antigen receptor (CAR) protein to a cell population comprising T cells derived from a mammal by a transposon method to obtain a genetically modified cell population; b) providing an endogenous cell population derived from the mammal expressing a protein that binds to the CAR; and c) coculturing the genetically modified cell population of the step a) and the endogenous cell population of the step b).

公开(公告)号：EP3766977A1

公开(公告)日：2021-01-20

申请号：EP19766785.0

申请日：2019-03-15

申请人： Shinshu University

发明人： NAKAZAWA, Yozo ,  HASEGAWA, Aiko ,  TANAKA, Miyuki ,  NAKANO, Shigeru ,  NARIMATSU, Shogo

IPC分类号： C12N15/62 ,  A61K35/12 ,  A61P35/02 ,  C12N5/10 ,  C12N15/12 ,  C12N15/13 ,  C12N15/27 ,  C12N15/85

摘要： It is intended to produce a cell expressing a mutant chimeric antigen receptor (CAR) having excellent cytotoxicity to target cells. The present invention provides a genetically modified cell having introduced thereinto a polynucleotide encoding a chimeric antigen receptor (CAR) protein having a target binding domain that specifically binds to a human granulocyte-macrophage colony stimulating factor (GM-CSF) receptor, a transmembrane domain, and an intracellular signaling domain, wherein the target binding domain is a mutant having the substitution of glutamic acid at position 21 in the amino acid sequence shown in SEQ ID NO: 1 with another amino acid.