公开(公告)号：EP3872087A1

公开(公告)日：2021-09-01

申请号：EP19877316.0

申请日：2019-10-25

申请人： Shinshu University

发明人： NAKAZAWA, Yozo ,  TANAKA, Miyuki ,  MOROKAWA, Hirokazu ,  NARIMATSU, Shogo

IPC分类号： C07K14/705 ,  C12N5/0783 ,  C12N5/10 ,  C12N15/12 ,  C12N15/63

摘要： An object of the present invention is to improve the efficiency of a method for producing chimeric antigen receptor (CAR)-expressing cells. The present invention provides a method for producing genetically modified mammalian cells, comprising the steps of: a) introducing a polynucleotide encoding a chimeric antigen receptor (CAR) protein to a cell population comprising T cells derived from a mammal by a transposon method to obtain a genetically modified cell population; b) providing an endogenous cell population derived from the mammal expressing a protein that binds to the CAR; and c) coculturing the genetically modified cell population of the step a) and the endogenous cell population of the step b).

公开(公告)号：EP4006148A1

公开(公告)日：2022-06-01

申请号：EP20847793.5

申请日：2020-07-30

申请人： Shinshu University ,  Kyoto Prefectural Public University Corporation ,  BrightPath Biotherapeutics Co., Ltd.

发明人： NAKAZAWA, Yozo ,  YAGYU, Shigeki ,  TANAKA, Miyuki ,  NAKAMURA, Kayoko ,  OKADA, Masahiro ,  KONDO, Makoto ,  SHIGEURA, Tomokuni ,  HIROTA, Shogo

IPC分类号： C12N5/10 ,  C12N15/12 ,  C12N15/62 ,  C12N15/85 ,  A61K35/17

摘要： The present disclosure includes a method of producing a cell population containing Chimeric Antigen Receptor (CAR)-expressing immune cells, comprising co-culturing CAR-expressing immune cells and cells expressing a target antigen of the CAR, wherein the CAR-expressing immune cells are cells into which a CAR gene has been introduced and the target antigen-expressing cells are normal blood cells that have been engineered to express the target antigen.

公开(公告)号：EP3766977A1

公开(公告)日：2021-01-20

申请号：EP19766785.0

申请日：2019-03-15

申请人： Shinshu University

发明人： NAKAZAWA, Yozo ,  HASEGAWA, Aiko ,  TANAKA, Miyuki ,  NAKANO, Shigeru ,  NARIMATSU, Shogo

IPC分类号： C12N15/62 ,  A61K35/12 ,  A61P35/02 ,  C12N5/10 ,  C12N15/12 ,  C12N15/13 ,  C12N15/27 ,  C12N15/85

摘要： It is intended to produce a cell expressing a mutant chimeric antigen receptor (CAR) having excellent cytotoxicity to target cells. The present invention provides a genetically modified cell having introduced thereinto a polynucleotide encoding a chimeric antigen receptor (CAR) protein having a target binding domain that specifically binds to a human granulocyte-macrophage colony stimulating factor (GM-CSF) receptor, a transmembrane domain, and an intracellular signaling domain, wherein the target binding domain is a mutant having the substitution of glutamic acid at position 21 in the amino acid sequence shown in SEQ ID NO: 1 with another amino acid.

公开(公告)号：EP4428229A1

公开(公告)日：2024-09-11

申请号：EP22889998.5

申请日：2022-11-02

申请人： AGC INC. ,  Kyoto Prefectural Public University Corporation ,  Shinshu University

发明人： KISHIMOTO, Megumi ,  SHIMIZU, Yuto ,  KUBOTA, Susumu ,  YAGYU, Shigeki ,  SUEMATSU, Masaya ,  NAKAZAWA, Yozo ,  TANAKA, Miyuki

IPC分类号： C12N5/0783 ,  C12N15/11

CPC分类号： C12N15/11 ,  C12N5/06

摘要： The present invention provides a method for producing a chimeric antigen receptor T (CAR-T) cell, including a step of bead separating with a specific factor a T cell from a T cell source, wherein the specific factor is CD45RA+ or the like. The method enables production of CAR-T cells high antitumor property, and can produce highly functional CAR-T cells conveniently in a short time at a low cost.

公开(公告)号：EP4003304A1

公开(公告)日：2022-06-01

申请号：EP20753430.6

申请日：2020-07-22

申请人： KABUSHIKI KAISHA TOSHIBA ,  Shinshu University

发明人： AKAHOSHI, Eiichi ,  ISHIHARA, Mitsuko ,  NAKAZAWA, Yozo ,  MORITA, Daisuke

IPC分类号： A61K9/127 ,  A61K47/16 ,  A61K47/20 ,  A61K47/22 ,  C07C219/06 ,  C07C219/10 ,  C07C235/10 ,  C07C327/30 ,  C07C327/36 ,  C07C403/20 ,  C07C403/22 ,  C12N15/88 ,  C12N9/12 ,  C12N15/85 ,  C12N15/90 ,  C12P21/02 ,  A61K39/00 ,  C07K14/725

公开(公告)号：EP3978611A1

公开(公告)日：2022-04-06

申请号：EP20813406.4

申请日：2020-05-29

申请人： Shinshu University

发明人： NAKAZAWA, Yozo ,  SAITO, Shoji ,  YAGYU, Shigeki ,  NAKANO, Shigeru ,  MOMOSE, Takaki ,  HITOMI, Kenta

IPC分类号： C12N15/62

摘要： The present invention is intended to develop a chimeric antigen receptor (CAR) that is effective against solid tumor expressing anaplastic lymphoma kinase (ALK). The present invention provides a polynucleotide encoding a CAR protein comprising a target binding domain binding to an extracellular ligand binding region of ALK, a transmembrane domain, and an intracellular signaling domain. The target binding domain of the polynucleotide is selected from among FAM150A, FAM150B, and fragments thereof binding to the extracellular ligand binding region of ALK. The present invention also provides a genetically modified cell comprising the polynucleotide introduced thereinto.