公开(公告)号：EP2471929A1

公开(公告)日：2012-07-04

申请号：EP10016162.9

申请日：2010-12-29

申请人： Algenics

发明人： Carlier, Aude ,  Michel, Rémy ,  Dufourmantel, Nathalie ,  Cadoret, Jean-Paul ,  Lejeune, Alexandre

IPC分类号： C12N15/82 ,  C12P21/00 ,  C12R1/89

CPC分类号： C07K14/005 ,  C07K14/505 ,  C12N9/2402 ,  C12N15/8214 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2740/16122 ,  C12P21/005 ,  C12Q1/34 ,  C12Y302/01045 ,  G01N33/6842

摘要： The present invention concerns a transformed microalga producing a protein harboring a non-immunogenic "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide ; and (ii) The sequence of said protein,3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated

摘要翻译： 本发明涉及一种转化的微藻生产蛋白质中所述的转化微藻的质窝藏糖基化的非免疫原性的“高甘露糖”图案，worin 1）所述的转化微藻具有叶绿体内质网（CER）; 2）所述微藻已经用可操作地连接于启动子的核酸序列，所述核酸序列编码的氨基酸序列，其包含（i）至氨基末端二分拓扑基因信号（BTS）序列由至少一个信号肽随后的 通过转运肽; 和（ii）所述蛋白质的序列，3）。所述的xylosyltransferases和fucosyltransferases微藻还没有被失活; 4）acetylglycosyltransferase的N-I所述微藻尚未失活，优选的是N-acetylglycosyltranferases II，III，IV，V和VI，甘露糖苷酶II和Said微藻的糖基转移酶都没有灭活