公开(公告)号：EP2660323A1

公开(公告)日：2013-11-06

申请号：EP12003135.6

申请日：2012-05-02

申请人： Algenics

发明人： Carlier, Aude ,  Michel, Rémy ,  Lejeune, Alexandre ,  Cadoret, Jean-Paul

IPC分类号： C12N15/82

CPC分类号： C12N15/8258

摘要： The invention relates to a transformed Phaeodactylum tricornutum microalga comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, said transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media. Preferably, said therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content. The present invention also relates to a method for producing said therapeutic antibody, a functional fragment or a derivative thereof. The present invention also relates to the use of a transformed Phaeodactylum tricornutum microalga according to the invention for the production and the secretion of a therapeutic antibody, a functional fragment or a derivative thereof in the extracellular medium. Another object to the invention is a therapeutic antibody, a functional fragment or a derivative thereof produced and secreted in the extracellular medium of microalgae by a method according to the invention. Finally, another object of the invention concerns a pharmaceutical composition comprising said therapeutic antibody, functional fragment or derivative thereof.

摘要翻译： 本发明涉及一种包含可操作地连接于启动子的核酸序列的转化的三角褐指藻微藻，其中所述核酸序列编码包含（i）异源信号肽的氨基酸序列; 和（ii）治疗性抗体，功能片段或其衍生物，所述转化的微细胞表达分泌在细胞外培养基中的治疗性抗体，功能片段或衍生物。 优选地，所述治疗性抗体，其功能片段或衍生物具有增加的抗体依赖性细胞介导的细胞毒性（ADCC）和低岩藻糖含量。 本发明还涉及生产所述治疗性抗体的方法，功能片段或其衍生物。 本发明还涉及根据本发明的经转化的三叶黄单胞菌微藻在细胞外培养基中产生和分泌治疗性抗体，功能片段或其衍生物的用途。 本发明的另一个目的是通过根据本发明的方法在微藻的细胞外培养基中产生和分泌的治疗性抗体，功能片段或其衍生物。 最后，本发明的另一个目的涉及包含所述治疗性抗体，其功能片段或衍生物的药物组合物。

公开(公告)号：EP2844760A1

公开(公告)日：2015-03-11

申请号：EP13727801.6

申请日：2013-05-02

申请人： Algenics

发明人： CARLIER, Aude ,  MICHEL, Rémy ,  LEJEUNE, Alexandre ,  CADORET, Jean-Paul

IPC分类号： C12N15/82

CPC分类号： C07K16/2887 ,  C07K16/2863 ,  C07K2317/13 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/76 ,  C12N1/12 ,  C12N15/79 ,  C12N15/8258

摘要： The invention relates to a transformed microalga comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, said transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and said microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, said therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content. The present invention also relates to a method for producing said therapeutic antibody, a functional fragment or a derivative thereof, to the use of a transformed microalga according to the invention for the production and the secretion of a therapeutic antibody, a functional fragment or a derivative thereof in the extracellular medium, to a therapeutic antibody, a functional fragment or a derivative thereof produced and secreted in the extracellular medium of microalgae by a method according to the invention and to a pharmaceutical composition comprising said therapeutic antibody, functional fragment or derivative thereof.

公开(公告)号：EP2598648A1

公开(公告)日：2013-06-05

申请号：EP11741110.8

申请日：2011-07-27

申请人： Universite de Rouen ,  Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ,  Algenics

发明人： BARDOR, Muriel ,  LOUVET, Romain ,  SAINT-JEAN, Bruno ,  BUREL, Carole ,  BAIET, Bérangère ,  CADORET, Jean-Paul ,  LEROUGE, Patrice ,  MICHEL, Rémy ,  CARLIER, Aude

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24 ,  C12N1/13 ,  C07K14/505

CPC分类号： C12N15/8243 ,  C07K14/505 ,  C12N9/1051 ,  C12N9/2402 ,  C12P21/005 ,  C12Y302/01096 ,  Y02P20/52

摘要： The present invention concerns transformed Phaeodactylum tricomutum comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an a-Mannosidase II and wherein at least one β-Ν-acetylglucosaminidase of said transformed Phaeodactylum tricomutum has been inactivated; a method for producing a glycosylated polypeptide, said method comprising the steps of (i) culturing a transformed P. tricomutum as defined previously and (ii) purifying said polypeptide that is expressed and glycosylated in said transformed P. tricomutum; and the use of such a transformed P. tricomutum for producing a glycosylated polypeptide.

摘要翻译： 本发明涉及包含可操作地连接到启动子的核酸序列的转化的三叶黄瓜，其中所述核酸序列编码N-乙酰葡糖胺基转移酶I和/或± - 甘露糖苷酶II，并且其中至少一个所述转化的2-N-乙酰氨基葡糖苷酶 三角褐指藻已灭活; 一种制备糖基化多肽的方法，所述方法包括以下步骤：（i）培养如前所定义的转化的三角褐豆胡桃，和（ii）纯化在所述转化的三角褐豆中表达和糖基化的所述多肽; 以及使用这种经转化的三角褐指藻来生产糖基化多肽。

公开(公告)号：EP2471929A1

公开(公告)日：2012-07-04

申请号：EP10016162.9

申请日：2010-12-29

申请人： Algenics

发明人： Carlier, Aude ,  Michel, Rémy ,  Dufourmantel, Nathalie ,  Cadoret, Jean-Paul ,  Lejeune, Alexandre

IPC分类号： C12N15/82 ,  C12P21/00 ,  C12R1/89

CPC分类号： C07K14/005 ,  C07K14/505 ,  C12N9/2402 ,  C12N15/8214 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2740/16122 ,  C12P21/005 ,  C12Q1/34 ,  C12Y302/01045 ,  G01N33/6842

摘要： The present invention concerns a transformed microalga producing a protein harboring a non-immunogenic "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide ; and (ii) The sequence of said protein,3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated

摘要翻译： 本发明涉及一种转化的微藻生产蛋白质中所述的转化微藻的质窝藏糖基化的非免疫原性的“高甘露糖”图案，worin 1）所述的转化微藻具有叶绿体内质网（CER）; 2）所述微藻已经用可操作地连接于启动子的核酸序列，所述核酸序列编码的氨基酸序列，其包含（i）至氨基末端二分拓扑基因信号（BTS）序列由至少一个信号肽随后的 通过转运肽; 和（ii）所述蛋白质的序列，3）。所述的xylosyltransferases和fucosyltransferases微藻还没有被失活; 4）acetylglycosyltransferase的N-I所述微藻尚未失活，优选的是N-acetylglycosyltranferases II，III，IV，V和VI，甘露糖苷酶II和Said微藻的糖基转移酶都没有灭活

公开(公告)号：EP2444495A1

公开(公告)日：2012-04-25

申请号：EP10013808.0

申请日：2010-10-20

申请人： Algenics

发明人： Lejeune, Alexandre ,  Michel, Rémy ,  Cadoret, Jean-Paul ,  Carlier, Aude

IPC分类号： C12N15/82 ,  C12P21/02 ,  C07K14/505 ,  C07K14/55

CPC分类号： C12P21/00 ,  C07K14/505 ,  C07K14/55 ,  C12N15/8257 ,  C12P21/02

摘要： The present invention concerns a transformed diatom comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide and (ii) a polypeptide, said heterologous signal peptide leading to the secretion of said polypeptide in the extracellular medium of said transformed diatom ; a method for producing a polypeptide which is secreted in the extracellular medium, said method comprising the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of said culture and (iii) purifying the secreted polypeptide in said extracellular medium; and use of said transformed diatom for the secretion of a polypeptide in the extracellular medium.

摘要翻译： 本发明涉及包含与启动子有效连接的核酸序列的转化硅藻，其中所述核酸序列编码氨基酸序列，其包含（i）异源信号肽和（ii）多肽，所述异源信号肽导致 所述多肽在所述转化的硅藻的细胞外培养基中的分泌; 一种生产分泌在细胞外介质中的多肽的方法，所述方法包括以下步骤：（i）培养转化的硅藻，（ii）收获所述培养物的细胞外培养物，和（iii）纯化所述细胞外培养基中分泌的多肽 ; 以及使用所述转化的硅藻在细胞外培养基中分泌多肽。

公开(公告)号：EP2598648B1

公开(公告)日：2017-11-08

申请号：EP11741110.8

申请日：2011-07-27

申请人： Université de Rouen ,  Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ,  Algenics

发明人： BARDOR, Muriel ,  LOUVET, Romain ,  SAINT-JEAN, Bruno ,  BUREL, Carole ,  BAIET, Bérangère ,  CADORET, Jean-Paul ,  LEROUGE, Patrice ,  MICHEL, Rémy ,  CARLIER, Aude

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24 ,  C12N1/13 ,  C07K14/505

CPC分类号： C12N15/8243 ,  C07K14/505 ,  C12N9/1051 ,  C12N9/2402 ,  C12P21/005 ,  C12Y302/01096 ,  Y02P20/52

公开(公告)号：EP2660324A1

公开(公告)日：2013-11-06

申请号：EP12003136.4

申请日：2012-05-02

申请人： Algenics

发明人： Carlier, Aude ,  Michel, Rémy ,  Lejeune, Alexandre

IPC分类号： C12N15/82

CPC分类号： C12N15/8258

摘要： The invention relates to a transformed microalga comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, said transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and said microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, said therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content. The present invention also relates to a method for producing said therapeutic antibody, a functional fragment or a derivative thereof, to the use of a transformed microalga according to the invention for the production and the secretion of a therapeutic antibody, a functional fragment or a derivative thereof in the extracellular medium, to a therapeutic antibody, a functional fragment or a derivative thereof produced and secreted in the extracellular medium of microalgae by a method according to the invention and to a pharmaceutical composition comprising said therapeutic antibody, functional fragment or derivative thereof.

摘要翻译： 本发明涉及包含可操作地连接到启动子的核酸序列的转化微藻，其中所述核酸序列编码氨基酸序列，其包含（i）异源信号肽; 和（ii）治疗性抗体，功能片段或其衍生物，所述转化的微藻表达分泌在细胞外介质中的治疗性抗体，功能片段或衍生物，所述微藻选自除Volvocales之外的绿藻，以及红藻， 铬酸盐和黑体。 优选地，所述治疗性抗体，其功能片段或衍生物具有增加的抗体依赖性细胞介导的细胞毒性（ADCC）和低岩藻糖含量。 本发明还涉及使用根据本发明的转化微藻用于产生和分泌治疗性抗体，功能片段或衍生物的所述治疗性抗体，功能片段或其衍生物的制备方法 通过本发明的方法在细藻体的细胞外培养基中产生和分泌的治疗性抗体，功能片段或衍生物，以及包含所述治疗性抗体，功能片段或衍生物的药物组合物。

公开(公告)号：EP2658980A1

公开(公告)日：2013-11-06

申请号：EP11826113.0

申请日：2011-12-28

申请人： Algenics

发明人： CARLIER, Aude ,  MICHEL, Rémy ,  CADORET, Jean-Paul ,  LEJEUNE, Alexandre ,  DUFOURMANTEL, Nathalie

IPC分类号： C12N15/82 ,  C12P21/00 ,  C12R1/89

CPC分类号： C07K14/005 ,  C07K14/505 ,  C12N9/2402 ,  C12N15/8214 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2740/16122 ,  C12P21/005 ,  C12Q1/34 ,  C12Y302/01045 ,  G01N33/6842

摘要： The present invention concerns a transformed microalga producing a protein harboring a "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of said protein, 3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated.

公开(公告)号：EP2630243A1

公开(公告)日：2013-08-28

申请号：EP11787596.3

申请日：2011-10-20

申请人： Algenics

发明人： LEJEUNE, Alexandre ,  MICHEL, Rémy ,  CADORET, Jean-Paul ,  CARLIER, Aude

IPC分类号： C12N15/82 ,  C12P21/02 ,  C07K14/505 ,  C07K14/55

CPC分类号： C12P21/00 ,  C07K14/505 ,  C07K14/55 ,  C12N15/8257 ,  C12P21/02

摘要： The present invention concerns a transformed diatom comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide and (ii) a polypeptide, said heterologous signal peptide leading to the secretion of said polypeptide in the extracellular medium of said transformed diatom; a method for producing a polypeptide which is secreted in the extracellular medium, said method comprising the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of said culture and (iii) purifying the secreted polypeptide in said extracellular medium; and use of said transformed diatom for the secretion of a polypeptide in the extracellular medium.