公开(公告)号：EP2844760A1

公开(公告)日：2015-03-11

申请号：EP13727801.6

申请日：2013-05-02

申请人： Algenics

发明人： CARLIER, Aude ,  MICHEL, Rémy ,  LEJEUNE, Alexandre ,  CADORET, Jean-Paul

IPC分类号： C12N15/82

CPC分类号： C07K16/2887 ,  C07K16/2863 ,  C07K2317/13 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/76 ,  C12N1/12 ,  C12N15/79 ,  C12N15/8258

摘要： The invention relates to a transformed microalga comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, said transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and said microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, said therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content. The present invention also relates to a method for producing said therapeutic antibody, a functional fragment or a derivative thereof, to the use of a transformed microalga according to the invention for the production and the secretion of a therapeutic antibody, a functional fragment or a derivative thereof in the extracellular medium, to a therapeutic antibody, a functional fragment or a derivative thereof produced and secreted in the extracellular medium of microalgae by a method according to the invention and to a pharmaceutical composition comprising said therapeutic antibody, functional fragment or derivative thereof.

公开(公告)号：EP2658980A1

公开(公告)日：2013-11-06

申请号：EP11826113.0

申请日：2011-12-28

申请人： Algenics

发明人： CARLIER, Aude ,  MICHEL, Rémy ,  CADORET, Jean-Paul ,  LEJEUNE, Alexandre ,  DUFOURMANTEL, Nathalie

IPC分类号： C12N15/82 ,  C12P21/00 ,  C12R1/89

CPC分类号： C07K14/005 ,  C07K14/505 ,  C12N9/2402 ,  C12N15/8214 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2740/16122 ,  C12P21/005 ,  C12Q1/34 ,  C12Y302/01045 ,  G01N33/6842

摘要： The present invention concerns a transformed microalga producing a protein harboring a "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of said protein, 3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated.

公开(公告)号：EP2630243A1

公开(公告)日：2013-08-28

申请号：EP11787596.3

申请日：2011-10-20

申请人： Algenics

发明人： LEJEUNE, Alexandre ,  MICHEL, Rémy ,  CADORET, Jean-Paul ,  CARLIER, Aude

IPC分类号： C12N15/82 ,  C12P21/02 ,  C07K14/505 ,  C07K14/55

CPC分类号： C12P21/00 ,  C07K14/505 ,  C07K14/55 ,  C12N15/8257 ,  C12P21/02

摘要： The present invention concerns a transformed diatom comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide and (ii) a polypeptide, said heterologous signal peptide leading to the secretion of said polypeptide in the extracellular medium of said transformed diatom; a method for producing a polypeptide which is secreted in the extracellular medium, said method comprising the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of said culture and (iii) purifying the secreted polypeptide in said extracellular medium; and use of said transformed diatom for the secretion of a polypeptide in the extracellular medium.