公开(公告)号：EP2844760A1

公开(公告)日：2015-03-11

申请号：EP13727801.6

申请日：2013-05-02

申请人： Algenics

发明人： CARLIER, Aude ,  MICHEL, Rémy ,  LEJEUNE, Alexandre ,  CADORET, Jean-Paul

IPC分类号： C12N15/82

CPC分类号： C07K16/2887 ,  C07K16/2863 ,  C07K2317/13 ,  C07K2317/24 ,  C07K2317/732 ,  C07K2317/76 ,  C12N1/12 ,  C12N15/79 ,  C12N15/8258

摘要： The invention relates to a transformed microalga comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide; and (ii) a therapeutic antibody, a functional fragment or a derivative thereof, said transformed microalga expressing the therapeutic antibody, functional fragment or derivative thereof secreted in the extracellular media and said microalga being selected among green algae except Volvocales, and among red algae, chromalveolates, and euglenids. Preferably, said therapeutic antibody, functional fragment or derivative thereof has an increased antibody-dependant cell-mediated cytotoxicity (ADCC) and a low fucose content. The present invention also relates to a method for producing said therapeutic antibody, a functional fragment or a derivative thereof, to the use of a transformed microalga according to the invention for the production and the secretion of a therapeutic antibody, a functional fragment or a derivative thereof in the extracellular medium, to a therapeutic antibody, a functional fragment or a derivative thereof produced and secreted in the extracellular medium of microalgae by a method according to the invention and to a pharmaceutical composition comprising said therapeutic antibody, functional fragment or derivative thereof.

公开(公告)号：EP2598648A1

公开(公告)日：2013-06-05

申请号：EP11741110.8

申请日：2011-07-27

申请人： Universite de Rouen ,  Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ,  Algenics

发明人： BARDOR, Muriel ,  LOUVET, Romain ,  SAINT-JEAN, Bruno ,  BUREL, Carole ,  BAIET, Bérangère ,  CADORET, Jean-Paul ,  LEROUGE, Patrice ,  MICHEL, Rémy ,  CARLIER, Aude

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24 ,  C12N1/13 ,  C07K14/505

CPC分类号： C12N15/8243 ,  C07K14/505 ,  C12N9/1051 ,  C12N9/2402 ,  C12P21/005 ,  C12Y302/01096 ,  Y02P20/52

摘要： The present invention concerns transformed Phaeodactylum tricomutum comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an a-Mannosidase II and wherein at least one β-Ν-acetylglucosaminidase of said transformed Phaeodactylum tricomutum has been inactivated; a method for producing a glycosylated polypeptide, said method comprising the steps of (i) culturing a transformed P. tricomutum as defined previously and (ii) purifying said polypeptide that is expressed and glycosylated in said transformed P. tricomutum; and the use of such a transformed P. tricomutum for producing a glycosylated polypeptide.

摘要翻译： 本发明涉及包含可操作地连接到启动子的核酸序列的转化的三叶黄瓜，其中所述核酸序列编码N-乙酰葡糖胺基转移酶I和/或± - 甘露糖苷酶II，并且其中至少一个所述转化的2-N-乙酰氨基葡糖苷酶 三角褐指藻已灭活; 一种制备糖基化多肽的方法，所述方法包括以下步骤：（i）培养如前所定义的转化的三角褐豆胡桃，和（ii）纯化在所述转化的三角褐豆中表达和糖基化的所述多肽; 以及使用这种经转化的三角褐指藻来生产糖基化多肽。

公开(公告)号：EP2598648B1

公开(公告)日：2017-11-08

申请号：EP11741110.8

申请日：2011-07-27

申请人： Université de Rouen ,  Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ,  Algenics

发明人： BARDOR, Muriel ,  LOUVET, Romain ,  SAINT-JEAN, Bruno ,  BUREL, Carole ,  BAIET, Bérangère ,  CADORET, Jean-Paul ,  LEROUGE, Patrice ,  MICHEL, Rémy ,  CARLIER, Aude

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24 ,  C12N1/13 ,  C07K14/505

CPC分类号： C12N15/8243 ,  C07K14/505 ,  C12N9/1051 ,  C12N9/2402 ,  C12P21/005 ,  C12Y302/01096 ,  Y02P20/52

公开(公告)号：EP2658980A1

公开(公告)日：2013-11-06

申请号：EP11826113.0

申请日：2011-12-28

申请人： Algenics

发明人： CARLIER, Aude ,  MICHEL, Rémy ,  CADORET, Jean-Paul ,  LEJEUNE, Alexandre ,  DUFOURMANTEL, Nathalie

IPC分类号： C12N15/82 ,  C12P21/00 ,  C12R1/89

CPC分类号： C07K14/005 ,  C07K14/505 ,  C12N9/2402 ,  C12N15/8214 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2740/16122 ,  C12P21/005 ,  C12Q1/34 ,  C12Y302/01045 ,  G01N33/6842

摘要： The present invention concerns a transformed microalga producing a protein harboring a "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of said protein, 3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated.

公开(公告)号：EP2630243A1

公开(公告)日：2013-08-28

申请号：EP11787596.3

申请日：2011-10-20

申请人： Algenics

发明人： LEJEUNE, Alexandre ,  MICHEL, Rémy ,  CADORET, Jean-Paul ,  CARLIER, Aude

IPC分类号： C12N15/82 ,  C12P21/02 ,  C07K14/505 ,  C07K14/55

CPC分类号： C12P21/00 ,  C07K14/505 ,  C07K14/55 ,  C12N15/8257 ,  C12P21/02

摘要： The present invention concerns a transformed diatom comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an amino acid sequence comprising (i) an heterologous signal peptide and (ii) a polypeptide, said heterologous signal peptide leading to the secretion of said polypeptide in the extracellular medium of said transformed diatom; a method for producing a polypeptide which is secreted in the extracellular medium, said method comprising the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of said culture and (iii) purifying the secreted polypeptide in said extracellular medium; and use of said transformed diatom for the secretion of a polypeptide in the extracellular medium.