公开(公告)号：EP2935566A2

公开(公告)日：2015-10-28

申请号：EP13864074.3

申请日：2013-12-23

申请人： Algenol Biofuels, Inc.

发明人： WANG, Kui ,  SHI, Tuo ,  PIVEN, Irina ,  INABA, Masami ,  ULICZKA, Frank ,  KRAMER, Dan ,  ENKE, Heike ,  BAIER, Kerstin ,  FRIEDRICH, Alexandra ,  DUEHRING, Ulf

IPC分类号： C12N1/21 ,  C12N15/63

CPC分类号： C12P7/06 ,  C07K14/195 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12P7/065 ,  C12R1/01 ,  Y02E50/17

摘要： A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.

公开(公告)号：EP2723872A1

公开(公告)日：2014-04-30

申请号：EP12729154.0

申请日：2012-06-25

申请人： Algenol Biofuels Inc.

发明人： DUEHRING, Ulf ,  LEE, James ,  BAIER, Kerstin

IPC分类号： C12N15/74 ,  C12N15/65 ,  C12P5/02 ,  C12P7/06 ,  C12P5/00

CPC分类号： C12N15/8243 ,  C12N15/74 ,  C12P5/007 ,  C12P5/026 ,  C12P7/065 ,  Y02E50/17

摘要： One embodiment of the invention provides a genetically enhanced cyanobacterium producing a first chemical compound comprising: -a genetically enhanced genome with a first gene inactivation in a first essential or conditionally essential gene of the cyanobacterium, and -a first extrachromosomal plasmid harboring the first essential or conditionally essential gene and at least one first production gene for production of the first chemical compound, -wherein the cyanobacterium lacks a functional gene conferring biocide resistance. These cyanobacteria can produce a first valuable chemical compound in long-term cultures without the need to employ genes conferring biocide resistance.

公开(公告)号：EP2798056A1

公开(公告)日：2014-11-05

申请号：EP12816065.2

申请日：2012-12-21

申请人： Algenol Biofuels Inc.

发明人： DUEHRING, Ulf ,  BAIER, Kerstin ,  GERMER, Frauke ,  SHI, Tuo

IPC分类号： C12N1/12

CPC分类号： C12P7/06 ,  C12N1/12 ,  C12N15/74 ,  Y02E50/17

摘要： One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising: at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co
2+ or Zn
2+ -inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

公开(公告)号：EP2960334A1

公开(公告)日：2015-12-30

申请号：EP15181279.9

申请日：2012-12-21

申请人： Algenol Biofuels, Inc.

发明人： FRIEDRICH, Alexandra ,  DUEHRING, Ulf ,  BAIER, Kerstin ,  ENKE, Heike ,  KRAMER, Dan

IPC分类号： C12N15/74 ,  C12P7/06

CPC分类号： C12P7/065 ,  C12N15/52 ,  C12N15/74 ,  C12N15/8243 ,  Y02E50/17

摘要： This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium.

摘要翻译： 本发明提供了生产感兴趣的化学化合物的代谢增强的蓝藻，具有编码生物催化剂首先用于产生第一化学化合物中的至少两个第一生产的​​基因。 一个的两个第一生产基因是为第一生产基因的第一启动子的转录控制之下，而其他的两个第一生产基因是为第一生产基因的第二启动子的转录控制之下。 所述第一启动子和第二启动子是不同的条件下分别诱导，并且所述至少两个第一生物催化剂催化相同的化学反应。 代谢增强蓝藻gemäß到本发明允许延长生产第一化学化合物。 本发明的进一步规定包括，除其他外，用于产生代谢增强的蓝细菌和用于产生通过培养代谢增强的蓝细菌的第一化学化合物的方法的方法。

公开(公告)号：EP2935566B1

公开(公告)日：2018-06-06

申请号：EP13864074.3

申请日：2013-12-23

申请人： Algenol Biofuels, Inc.

发明人： WANG, Kui ,  SHI, Tuo ,  PIVEN, Irina ,  INABA, Masami ,  ULICZKA, Frank ,  KRAMER, Dan ,  ENKE, Heike ,  BAIER, Kerstin ,  FRIEDRICH, Alexandra ,  DUEHRING, Ulf

IPC分类号： C12N1/21 ,  C12N9/04 ,  C12N9/88 ,  C07K14/195 ,  C12N15/52 ,  C12N15/74 ,  C12P7/06 ,  C12R1/01

CPC分类号： C12P7/06 ,  C07K14/195 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12P7/065 ,  C12R1/01 ,  Y02E50/17

摘要： A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyano1, is disclosed. The enhanced Cyanobacterium sp. ABICyano1 produces a compound or compounds of interest.