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    METHOD OF AND COMPOSITE FOR FLUORESCENT IN SITU HYBRIDIZATION
    2.
    发明公开
    METHOD OF AND COMPOSITE FOR FLUORESCENT IN SITU HYBRIDIZATION 审中-公开
    荧光原位杂交法和混合材料THEREFOR

    公开(公告)号：EP1100965A1

    公开(公告)日：2001-05-23

    申请号：EP99935850.0

    申请日：1999-07-23

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： BAR-AM, Irit ,  JANISH, Cathy ,  GARINI, Yuval ,  CABIB, Dario ,  BUCKWALD, Robert, A.

    IPC: C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6841 ,  C12Q2527/125 ,  C12Q2563/107 ,  C12Q2527/101 ,  C12Q2525/151

    Abstract: A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (f) analyzing and presenting images of the now hybridized chromosome spread.

    METHOD FOR CHROMOSOMES CLASSIFICATION
    4.
    发明公开
    METHOD FOR CHROMOSOMES CLASSIFICATION 失效
    分类程序染色体

    公开(公告)号：EP0920537A1

    公开(公告)日：1999-06-09

    申请号：EP98913120.0

    申请日：1998-03-27

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： GARINI, Yuval ,  KATZIR, Nir ,  WINE, David ,  CABIB, Dario

    IPC: G01N33 ,  C12M1 ,  C12M3 ,  C12N15 ,  C12Q1 ,  G01J3 ,  G01N1 ,  G01N21 ,  G01N37 ,  G02B21 ,  G06K9

    CPC classification number: G01J3/12 ,  C12Q1/6816 ,  G01J3/02 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/0014 ,  G06K9/76

    Abstract: A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

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