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1.发明公开METHOD FOR IMMOBILIZING TEMPERATURE-RESPONSIVE PROTEIN A 审中-公开VERFAHREN ZUR IMMOBILISIERTE DES TEMPERATURREAKTIVEN蛋白A
公开(公告)号：EP2657254A1
公开(公告)日：2013-10-30
申请号：EP11850738.3
申请日：2011-12-26
CPC分类号： C07K14/31 , B01D15/3809 , B01D15/3876 , B01J20/261 , B01J20/286 , B01J20/321 , B01J20/3219 , B01J20/3274 , B01J2220/86
摘要： A method for manufacturing a carrier having temperature responsive protein A, which is protein A mutated such that a binding property to an antibody changes depending upon temperature, immobilized thereto, wherein the temperature responsive protein A has the binding property to the antibody in a first temperature region and no binding property to the antibody in a second temperature region; and the method comprises a step of immobilizing the temperature responsive protein A to a carrier surface at a temperature within the first temperature region in which the temperature responsive protein A has the binding property to the antibody.
摘要翻译： 一种制造具有温度敏感性蛋白A的载体的方法，其是突变的蛋白A，使得与抗体的结合性随固化的温度而变化，其中温度敏感性蛋白A在第一温度下具有与抗体的结合性 并且在第二温度区域对抗体没有结合性; 并且该方法包括在温度响应蛋白A具有与抗体结合性的第一温度区域内的温度下将温度响应蛋白A固定在载体表面上的步骤。
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公开(公告)号：EP3173783B1
公开(公告)日：2019-02-27
申请号：EP15824285.9
申请日：2015-07-23
发明人： TANIGUCHI, Hiroki , KOGUMA, Ichiro
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3.发明公开CARRIER FOR POSITIVE-ION-EXCHANGE CHROMATOGRAPHY AND METHOD FOR USING SAME 审中-公开用于阳离子交换色谱的载体和使用该载体的方法
公开(公告)号：EP3173783A1
公开(公告)日：2017-05-31
申请号：EP15824285.9
申请日：2015-07-23
发明人： TANIGUCHI, Hiroki , KOGUMA, Ichiro
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01J39/19 , B01J39/26 , B01J47/014 , B01J47/12 , C07K1/22 , C07K16/00 , C07K2317/21 , C08J5/2243 , C08J7/18 , C08J9/36 , C08J2323/06 , G01N30/02 , G01N30/88 , G01N30/96
摘要： The present invention provides a cation-exchange chromatographic support, comprising a membrane matrix and a copolymer immobilized on the surface of the membrane matrix, wherein the copolymer comprises a (meth)acrylamide-based compound and/or a (meth)acrylate-based compound as monomer units, and the support has one or more species of cation-exchange groups including at least a weak cation-exchange group at a density higher than 30 mmol/L per volume of the support.
摘要翻译： 本发明提供阳离子交换色谱支持体，其包含膜基质和固定在膜基质表面上的共聚物，其中所述共聚物包含基于（甲基）丙烯酰胺的化合物和/或基于（甲基）丙烯酸酯的化合物 作为单体单元，并且所述载体具有一种或多种阳离子交换基团，所述阳离子交换基团至少包括弱阳离子交换基团，其密度高于每体积载体30mmol / L。
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公开(公告)号：EP2889617A1
公开(公告)日：2015-07-01
申请号：EP13832456.1
申请日：2013-08-27
CPC分类号： C07K1/22 , B01D15/3809 , B01D15/3876 , G01N2030/8813 , B01D15/362
摘要： There is provided a method for purifying an antibody using temperature-responsive Protein A, wherein different buffer solutions are used in a washing step of washing a stationary phase having the temperature-responsive Protein A and in an elution step of eluting the antibody captured by the stationary phase, the method including the washing step of washing the stationary phase using a buffer solution at a temperature at which the temperature-responsive Protein A and the antibody are bound and having a first salt concentration, and the elution step of eluting the antibody captured by the stationary phase using a buffer solution at a temperature at which the antibody is released from the temperature-responsive Protein A and having a second salt concentration lower than the first salt concentration.
摘要翻译： 提供了使用温度敏感性蛋白A纯化抗体的方法，其中在洗涤具有温度敏感性蛋白A的固定相的洗涤步骤中使用不同的缓冲溶液，并且在洗脱步骤中洗脱由 所述方法包括在温度响应性蛋白A和抗体结合并具有第一盐浓度的温度下使用缓冲溶液洗涤固定相的洗涤步骤，并且洗脱所获得的抗体的洗脱步骤 通过在从温度敏感性蛋白A释放抗体的温度下使用缓冲溶液并具有低于第一盐浓度的第二盐浓度的固定相。
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公开(公告)号：EP1230970B1
公开(公告)日：2006-07-05
申请号：EP00969932.3
申请日：2000-10-20
CPC分类号： B32B27/08 , B01D67/003 , B01D69/02 , B01D71/34 , B01D2323/20 , B01D2325/02 , B01D2325/22 , B32B3/26 , B32B5/32 , B32B27/00 , B32B27/22 , B32B27/304 , B32B38/0036 , B32B2305/026 , B32B2307/306 , B32B2307/54 , H01M2/1653 , H01M2/1686 , H01M2300/0025 , Y10T428/249953 , Y10T428/249978 , Y10T428/249981
摘要： A heat-resistant microporous film which comprises a thermoplastic resin having a crystal melting point of 140 to 300 °C and has a layer structure formed in the direction of thickness, wherein the layer structure comprises 5 to 100 % of a layer defined by (A) below and 0 to 95 % of a layer defined by (B) below: (A) a layer having only micropores which are voids within a spherulite, and (B) a layer having micropores which are voids within a spherulite and micropores which are voids being present between spherulites.
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6.发明授权
公开(公告)号：EP2889617B1
公开(公告)日：2017-10-11
申请号：EP13832456.1
申请日：2013-08-27
CPC分类号： C07K1/22 , B01D15/3809 , B01D15/3876 , G01N2030/8813 , B01D15/362
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公开(公告)号：EP1413350B1
公开(公告)日：2011-12-14
申请号：EP02753217.5
申请日：2002-07-31
发明人： NAGOYA, Fujiharu , KOGUMA, Ichiro
CPC分类号： B01D67/0083 , B01D67/0011 , B01D67/0016 , B01D67/0018 , B01D67/003 , B01D67/0093 , B01D69/02 , B01D69/08 , B01D71/34 , B01D2323/385 , B01D2325/02 , B01D2325/022 , Y10T428/249958 , Y10T428/249961 , Y10T428/249964 , Y10T428/249978 , Y10T428/249979
摘要： A multilayer microporous film which comprises a thermoplastic resin and has a rough structure layer having a high proportion of open pores and a dense structure layer having a low proportion of open pores, wherein said rough structure layer is present in at least one surface portion of the film and has a thickness of 5.0 μm or more, said dense structure layer has a thickness accounting for 50 % or more of that of the total film, and said rough structure layer and said dense structure layer are integrated with each other.
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公开(公告)号：EP2987801B1
公开(公告)日：2019-06-12
申请号：EP14785266.9
申请日：2014-04-15
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公开(公告)号：EP2987801A1
公开(公告)日：2016-02-24
申请号：EP14785266.9
申请日：2014-04-15
IPC分类号： C07K1/18 , B01D15/04 , B01D15/08 , B01J20/24 , B01J20/26 , B01J20/281 , B01J20/30 , B01J20/34 , B01J39/04 , B01J39/26 , C07K1/22 , C08F291/00 , G01N30/26 , G01N30/54 , G01N30/88
CPC分类号： C07K1/22 , B01D15/362 , B01D15/3809 , B01J39/05 , B01J39/17 , B01J39/20 , B01J39/26 , B01J49/06 , C07K1/18 , C07K16/00 , G01N30/30 , G01N30/54 , G01N30/96 , G01N2030/8813
摘要： A method for purifying a biologically active substance from a solution mixture containing impurities and the biologically active substance, in which an ion exchange chromatography carrier comprising a matrix and a copolymer containing at least N-isopropylacrylamide as a monomer unit and immobilized to a surface of the matrix is used, and the solution mixture is allowed to flow through a container storing the carrier at a uniform temperature, thereby recovering the biologically active substance.
摘要翻译： 一种从含有杂质和生物活性物质的溶液混合物中纯化生物活性物质的方法，其中包含基质和至少含有N-异丙基丙烯酰胺作为单体单元的共聚物的离子交换色谱载体固定在 使溶液混合物在均匀的温度下流过容纳载体的容器，从而回收生物活性物质。
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10.发明公开TEMPERATURE-RESPONSIVE ADSORBENT HAVING STRONG CATION EXCHANGE GROUP, AND METHOD FOR PRODUCING SAME 审中-公开与制造具有强力阳离子交换基团和过程温度反应吸附
公开(公告)号：EP2653218A1
公开(公告)日：2013-10-23
申请号：EP11848385.8
申请日：2011-12-19
发明人： KOGUMA, Ichiro , SHIGEMATSU, Hiroki , OKUYAMA, Kazuo , OKANO, Teruo , AKIYAMA, Yoshikatsu , NAGASE, Kenichi
IPC分类号： B01J20/26 , B01J20/30 , B01J20/34 , C08F8/36 , C08F261/04
CPC分类号： B01J39/20 , B01J20/285 , B01J20/3276 , B01J20/3278 , B01J39/05 , B01J39/19 , B01J39/26 , C07K1/18 , C08F293/005 , C08F2438/01
摘要： Provided is a temperature responsive adsorbent prepared by immobilizing a copolymer containing at least N-isopropylacrylamide to a base material surface. The copolymer has at least a strong cation exchange group.
In addition, the copolymer contains the strong cation exchange group in an amount of 0.01 to 5 mol% relative to N-isopropylacrylamide in terms of monomer.摘要翻译： 提供了通过固定化含有至少N-异丙基于基材表面的共聚物而制备的温度响应吸附剂。 该共聚物具有至少一个强阳离子交换基团。 此外，该共聚物含有以相对于N-异丙基0点01分〜5摩尔％的量在单体而言强阳离子交换基团。
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