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公开(公告)号：EP1364205B1
公开(公告)日：2007-05-23
申请号：EP02713487.3
申请日：2002-01-28
申请人： Avigenics, Inc.
发明人： RAPP, Jeffrey, C.
IPC分类号： G01N33/00 , A01K67/027 , C12N5/00 , C12N5/02 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/85
CPC分类号： C12N15/8509 , A01K2207/15 , A01K2217/00 , A01K2227/30 , A01K2267/01 , C07K16/02 , C12N15/873 , C12N2799/022 , C12N2799/027 , C12N2840/203
摘要： The present invention relates generally to novel methods of producing transgenic chickens that generate antibodies or immunoglobulin polypeptides in whites of eggs. More specifically, one embodiment of the present invention relates to methods of inserting immunoglobulin-encoding transgenes into avian sperm cells for transfer to ova to generate transgenic zygotes. The transgenes may include at least two immunoglobulin-encoding nucleic acid sequences and an internal ribosome entry site (IRES) that allow the immunoglobulin polypeptides to be expressed by chicken cells and hence in egg whites.
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公开(公告)号：EP1313849B8
公开(公告)日：2007-03-07
申请号：EP01968166.7
申请日：2001-08-27
申请人： Avigenics, Inc.
IPC分类号： C12N15/00 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0273 , A01K2217/05 , A01K2217/075 , A01K2227/30 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N2770/24222
摘要： Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.
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公开(公告)号：EP1478751A2
公开(公告)日：2004-11-24
申请号：EP02725432.5
申请日：2002-03-29
申请人： Avigenics, Inc.
发明人： RAPP, Jeffrey, C.
IPC分类号： C12N15/11
CPC分类号： C12N15/85 , A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/30 , A01K2267/01 , C07K14/56 , C07K16/2818 , C07K2317/20 , C07K2317/21 , C07K2317/23 , C12N15/8509 , C12N2830/00 , C12N2830/46 , C12N2830/90
摘要： The present invention provides novel isolated nucleic acids comprising an avian nucleic acid sequence encoding a lysozyme gene expression control region. The isolated nucleic acid of the present invention is useful for reducing the chromosomal positional effect of a transgene operably linked to the lysozyme gene expression control region and transfected into a recipient cell and allows expression of an operably linked heterologous nucleic acid insert in a transfected avian cells such as, for example, an oviduct cell. The isolated avian lysozyme of the present invention may be operably linked with a selected nucleic acid insert encoding a polypeptide desired to be expressed in a transfected cell. The recombinant DNA of the present invention may further comprise a polyadenylation signal sequence or a chicken lysozyme 3' domain.
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公开(公告)号：EP2007803A1
公开(公告)日：2008-12-31
申请号：EP07751151.7
申请日：2007-02-20
IPC分类号： C07K14/535
CPC分类号： C07K14/535
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公开(公告)号：EP1313849B1
公开(公告)日：2006-06-07
申请号：EP01968166.7
申请日：2001-08-27
申请人： Avigenics, Inc.
IPC分类号： C12N15/00 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0273 , A01K2217/05 , A01K2217/075 , A01K2227/30 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N2770/24222
摘要： Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.
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公开(公告)号：EP1364205A1
公开(公告)日：2003-11-26
申请号：EP02713487.3
申请日：2002-01-28
申请人： Avigenics, Inc.
发明人： RAPP, Jeffrey, C.
IPC分类号： G01N33/00 , A01K67/027 , C12N5/00 , C12N5/02 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/85
CPC分类号： C12N15/8509 , A01K2207/15 , A01K2217/00 , A01K2227/30 , A01K2267/01 , C07K16/02 , C12N15/873 , C12N2799/022 , C12N2799/027 , C12N2840/203
摘要： The present invention relates generally to novel methods of producing transgenic chickens that generate antibodies or immunoglobulin polypeptides in whites of eggs. More specifically, one embodiment of the present invention relates to methods of inserting immunoglobulin-encoding transgenes into avian sperm cells for transfer to ova to generate transgenic zygotes. The transgenes may include at least two immunoglobulin-encoding nucleic acid sequences and an internal ribosome entry site (IRES) that allow the immunoglobulin polypeptides to be expressed by chicken cells and hence in egg whites.
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公开(公告)号：EP1812568A2
公开(公告)日：2007-08-01
申请号：EP05786634.5
申请日：2005-08-23
申请人： Avigenics, Inc.
IPC分类号： C12N15/11
CPC分类号： C12N15/1133 , C12N15/1131 , C12N2310/111 , C12N2310/14 , C12N2310/53
摘要： The invention relates to nucleotide sequences which encode therapeutic polynucleotides which correspond to one or more certain sequences in the genome of an avian pathogen. The invention also relates to transgenic avians whose cells contain such nucleotide sequences.
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公开(公告)号：EP1592773A2
公开(公告)日：2005-11-09
申请号：EP04704892.1
申请日：2004-01-23
IPC分类号： C12N1/00
CPC分类号： C12N15/86 , A01K2217/05 , A01K2227/30 , A01K2267/01 , C12N15/8509 , C12N15/902 , C12N2740/13043 , C12N2799/027 , C12N2830/008 , C12N2830/15 , C12N2830/40 , C12N2830/90 , C12N2840/20 , C12N2840/203
摘要： This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of avians in order to express the exogenous sequences to alter the phenotype of the avians or to produce desired proteins. In particular, transgenic avians are produced which express exogenous sequences in their oviducts and which deposit exogenous proteins into their eggs. Avian eggs that contain exogenous proteins are encompassed by this invention. The instant invention further provides novel forms of interferon and erythropoietin which are efficiently expressed in the oviduct of transgenic avians and deposited into avian eggs.
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公开(公告)号：EP1438401A2
公开(公告)日：2004-07-21
申请号：EP02775895.2
申请日：2002-09-18
申请人： Avigenics, Inc.
IPC分类号： C12N15/00
CPC分类号： A01K67/0275 , A01K2207/15 , A01K2217/00 , A01K2227/30 , A01K2267/01 , C07K16/00 , C07K2317/20 , C07K2317/23 , C12N15/8509 , C12N2799/027 , C12N2800/30 , C12N2830/008 , C12N2830/15 , C12N2830/46 , C12N2830/90 , C12N2840/203
摘要： This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.
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公开(公告)号：EP1313849A2
公开(公告)日：2003-05-28
申请号：EP01968166.7
申请日：2001-08-27
申请人： Avigenics, Inc.
IPC分类号： C12N15/00 , A01K67/027
CPC分类号： C12N15/8509 , A01K67/0273 , A01K2217/05 , A01K2217/075 , A01K2227/30 , A01K2267/01 , A01K2267/02 , C07K14/005 , C12N2770/24222
摘要： Methods for producing cloned non-human animals by nuclear transfer and by combinations of nuclear transfer and ovum transfer, employing near-infrared visualization of the recipient cell nucleus are described herein. Transgenic, knock-out, and knock-in avians are provided. Method for producing eggs which contain exogenous proteins are encompassed by the present invention. Thus, the instant invention satisfies the need for an effective route to the generation of cloned avians.
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