公开(公告)号：EP2606333A4

公开(公告)日：2015-03-25

申请号：EP11818860

申请日：2011-08-19

Applicant: BIO RAD LABORATORIES

Inventor： HENG XIN ,  PATT PAUL

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1436 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/645 ,  G01N2021/6421

Abstract: A flow cytometry system includes a flow element through which a cell is transported in a flowing fluid. The flow element includes a bore bounded by a wall. A light source is configured to illuminate the cell. An optical system receives light emanating from the cell and directs at least some of the received light to a light sensor. The optical system includes a numerical-aperture-increasing lens at a wall of the flow element. At least some of the received light passes through the numerical-aperture-increasing lens. The flow cytometry system may also include a beam splitter that directs two wavelength bands of the emanating light such that light in two wavelength band preferentially reach different sensing locations via different paths. The system may also include an optical element placed in one of the paths, shifting a focal location of the affected path to compensate for chromatic aberration of the numerical-aperture-increasing lens.

公开(公告)号：EP2643453A4

公开(公告)日：2014-06-11

申请号：EP11843656

申请日：2011-11-18

Applicant: BIO RAD LABORATORIES

Inventor： ARCHER ROBERT M ,  HSIUNG FRANK ,  PATT PAUL

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  G01J1/56 ,  G01J3/30 ,  G01N23/00 ,  G01N33/58

CPC classification number: G01N33/5094 ,  G01N15/0606 ,  G01N15/1463 ,  G01N2015/0288 ,  G01N2015/149

公开(公告)号：EP2467806A4

公开(公告)日：2016-06-01

申请号：EP10810688

申请日：2010-08-20

Applicant: BIO RAD LABORATORIES

Inventor： PATT PAUL

IPC: G01N15/14

CPC classification number: G01N15/14

公开(公告)号：EP2909613A4

公开(公告)日：2016-10-26

申请号：EP13846939

申请日：2013-10-17

Applicant: BIO RAD LABORATORIES

Inventor： BARICH JOHN ,  LEMAIRE-ADKINS RENEE ,  NEAZ DAVID ,  NOTCOVICH ARIEL ,  PATT PAUL ,  SHORT RYAN ,  SWIHART STEVEN ,  THRUSH EVAN

IPC: G01N27/26 ,  G01N21/64 ,  G01N27/447 ,  H04N3/14

CPC classification number: G01N21/63 ,  G01N21/6452 ,  G01N27/44726 ,  G01N2021/6484 ,  G01N2201/0833 ,  G01N2201/10 ,  G01N2201/103 ,  G01N2201/127 ,  H04N5/2624 ,  H04N5/349 ,  H04N5/3572 ,  H04N5/361

Abstract: Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.

公开(公告)号：EP2678660A4

公开(公告)日：2015-08-26

申请号：EP12749439

申请日：2012-02-21

Applicant: BIO RAD LABORATORIES

Inventor： HENG XIN ,  PATT PAUL

IPC: G01N21/00 ,  G01N15/14

CPC classification number: G01N15/1459 ,  G01N15/1434 ,  G01N2015/144

公开(公告)号：EP2473948A4

公开(公告)日：2014-07-09

申请号：EP10812708

申请日：2010-08-30

Applicant: BIO RAD LABORATORIES

Inventor： MCCOLLUM TOM ,  PATT PAUL ,  SHEN FRANK ,  CHU DANIEL Y ,  FLORY DON ,  GRIFFIN MIKE ,  HENG XIN ,  HEFNER ELI

IPC: G06K9/00 ,  C12M1/34 ,  G01N15/02 ,  G01N15/06 ,  G01N21/64 ,  G02B21/34 ,  G06T7/00

CPC classification number: C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 mum or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.