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    Somatotropins with alterations in the alpha-helix 1 region, and combinations with other mutations
    3.
    发明公开
    Somatotropins with alterations in the alpha-helix 1 region, and combinations with other mutations 失效
    生长激素受体激动剂1和Kombinationen mit anderen Mutationen。

    公开(公告)号：EP0496973A2

    公开(公告)日：1992-08-05

    申请号：EP91120396.6

    申请日：1991-11-28

    申请人： BIO-TECHNOLOGY GENERAL CORPORATION ,  AMERICAN CYANAMID COMPANY

    发明人： Fischer, Meir ,  Lebens, Michael Richard ,  Chaleff, Deborah Tardy

    IPC分类号： C12N15/18 ,  C07K13/00 ,  C07K3/08

    CPC分类号： C07K1/1133 ,  C07K14/61

    摘要： The present invention relates to somatotropin analogues with amino acid changes in the alpha-helix 1 regions of said somatotropins, alone or in combination with mutations in the alpha-helix 3 and/or alpha-helix 2 regions, plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptide also are provided.

    摘要翻译： 本发明涉及在所述生长激素的α-螺旋1区域中具有氨基酸变化的生长激素类似物，单独或与α-螺旋3和/或α-螺旋2区域中的突变组合，以及与 生长激素的天然氨基酸序列。 所得类似物在持续释放制剂中配制稳定，同时保持生物活性。 此外，还提供了对编码蛋白质和/或多肽的DNA进行定点诱变的方法。

    Somatotropins with alterations in the alpha-helix 3 region, alpha-helix 2 region combinations thereof, and in combination with other mutations
    7.
    发明公开
    Somatotropins with alterations in the alpha-helix 3 region, alpha-helix 2 region combinations thereof, and in combination with other mutations 失效
    与其3个组合在α螺旋的区域中的改进和组合与其他突变的生长激素。

    公开(公告)号：EP0488279A2

    公开(公告)日：1992-06-03

    申请号：EP91120390.9

    申请日：1991-11-28

    申请人： AMERICAN CYANAMID COMPANY

    发明人： Chaleff, Deborah Tardy

    IPC分类号： C12N15/18 ,  C07K13/00 ,  C12N15/10 ,  C07K3/08 ,  C07K15/00

    CPC分类号： C07K1/1133 ,  C07K14/61 ,  C12N15/102 ,  Y10S930/12

    摘要： The present invention relates to somatotropin analogues with amino acid changes in the α-helix 3 regions of said somatotropins, changes in the α-helix 2 regions, combinations thereof plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptides also are provided.

    摘要翻译： 本发明涉及生长激素与所述α-螺旋的氨基酸变化的类似物生长激素。所述的3个区域，在α-螺旋2个区域的变化，它们的组合加上其他更改生长激素的天然氨基酸序列的组合。 将所得的类似物是用于持续释放式蒸发散制剂稳定，同时保持生物活性。 此外，提供了用于进行上DNA编码的蛋白质和/或多肽，因此位点定向诱变的方法。

    Stabilization of somatotropins by modification of cysteine residues utilizing site directed mutagenesis or chemical derivatization
    8.
    发明公开
    Stabilization of somatotropins by modification of cysteine residues utilizing site directed mutagenesis or chemical derivatization 失效
    生长激素由半胱氨酸残基的修饰通过定点突变或化学衍生的稳定。

    公开(公告)号：EP0355460A2

    公开(公告)日：1990-02-28

    申请号：EP89113852.1

    申请日：1989-07-27

    申请人： AMERICAN CYANAMID COMPANY

    发明人： Cady, Susan Mancini ,  Logan, John Steele ,  Buckwalter, Brian Lee ,  Stockton, Gerald W. ,  Chaleff, Deborah Tardy

    IPC分类号： C12N15/18 ,  C12P21/02 ,  C07K3/08 ,  A61K37/36

    CPC分类号： C07K1/1133 ,  A61K38/00 ,  C07K14/61

    摘要： The present invention relates to novel modified or derivatized recombinant animal somatotropins. This invention also provides methods for stabilizing recombinant animal somatotropins by modification or deletion of the Cysteine residues utilizing site directed mutagenesis to replace from one to four of the cysteine amino acid residues of said somatotropins with one or more different amino acid residues or by derivatization of (1) both cysteine amino acid residues in the small loop of said somatotropin, both cysteine amino acid residues in the large loop or the four cysteine amino acid residues in both loops of said somatotropin.

    摘要翻译： 本发明涉及新颖的改性或衍生的重组动物生长激素。 所以本发明提供了用于稳定通过修饰或半胱氨酸残基利用位点定向诱变的缺失重组动物生长激素从一个与一个或多个不同的氨基酸残基，或通过衍生化替换到所述生长激素的半胱氨酸氨基酸残基的四种方法（ 1）两个半胱氨酸氨基酸残基在说生长激素的小环，在大环两个半胱氨酸氨基酸残基或所述生长激素两个环的4个半胱氨酸氨基酸残基。