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    CYCLIC PEPTIDE LIBRARIES AND METHODS OF USE THEREOF TO IDENTIFY BINDING MOTIFS
    4.
    发明公开
    CYCLIC PEPTIDE LIBRARIES AND METHODS OF USE THEREOF TO IDENTIFY BINDING MOTIFS 失效
    图书馆环肽及其用于标识结合基序

    公开(公告)号：EP0990156A1

    公开(公告)日：2000-04-05

    申请号：EP98923835.7

    申请日：1998-05-28

    申请人： Beth Israel Deaconess Medical Center

    发明人： LAI, Hung-sen ,  YAFFE, Michael, B. ,  SONGYANG, Zhou ,  CARRAWAY, Kermit, L., III ,  CANTLEY, Lewis, C.

    IPC分类号： G01N33/68 ,  C12Q1/48 ,  C12Q1/42 ,  C12Q1/37 ,  C07K1/14 ,  C07K4/00

    CPC分类号： C12Q1/37 ,  C07K1/047 ,  C07K7/64 ,  G01N2333/9121 ,  G01N2333/976

    摘要： Methods for determining an optimal binding motif for a binding compound are provided in which the binding compound is contacted with an oriented degenerate cyclic peptide library (ODCPL) under conditions which allow for interaction between the binding compound and the ODCPL such that a complex is formed between the binding compound and a subpopulation of library members capable of interacting with the binding compound. The subpopulation of library members capable of interacting with the binding compound is then separated from library members that are incapable of interacting with the binding compound. The subpopulation of library members capable of interacting with the binding compound is linearized to form a subpopulation of linearized library members. The amino acid sequence of the subpopulation of linearized library members is determined and an amino acid sequence motif is then determined for an interaction site of the binding compound, based upon the relative abundance of different amino acid residues at each degenerate position within the linearized library members. Oriented degenerate cyclic peptide libraries, and methods for purifying cyclic peptides from linear peptides, are also provided.