公开(公告)号：EP2490701A4

公开(公告)日：2013-09-25

申请号：EP10828771

申请日：2010-10-19

申请人： CELLULAR DYNAMICS INT INC

发明人： STANKEWICZ CASEY ,  RILEY MATT ,  BEARDSLEY NATHANIEL ,  WANG WEN BO ,  KATTMAN STEVEN

IPC分类号： A61K35/12 ,  A61K38/18 ,  A61P9/00 ,  A61P9/04 ,  C12N5/0735

CPC分类号： C12N5/0657 ,  A61K48/00 ,  C07H21/04 ,  C07K14/475 ,  C07K14/50 ,  C12N5/0606 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/48 ,  C12N2501/70 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2510/00

摘要： Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.