公开(公告)号：EP1571211A4

公开(公告)日：2006-03-22

申请号：EP03775858

申请日：2003-11-21

申请人： CHUGAI PHARMACEUTICAL CO LTD ,  PHARMALOGICALS RES PTE LTD

发明人： TSUCHIYA MASAYUKI ,  SUZUKI MASAMI ,  YOSHIDA KENJI ,  FUJII ETSUKO ,  MATSUBARA KOUICHI ,  TSUNODA HIROYUKI

IPC分类号： C12N15/12 ,  A61K39/395 ,  A61P35/00 ,  C07K16/00 ,  C07K16/18 ,  C07K16/30 ,  C07K16/32 ,  C12N1/21 ,  C12N15/09 ,  C12N15/13 ,  C12N15/70 ,  G01N33/53 ,  G01N33/547 ,  G01N33/577

CPC分类号： C07K16/00 ,  C07K16/30 ,  C07K16/3015 ,  C07K2317/21 ,  C07K2317/622

摘要： It is intended to provide a method of isolating a polynucleotide encoding an antibody against a lesion which involves the following steps: (a) the step of isolating B cells infiltrating into the target lesion; and (b) the step of obtaining a polynucleotide encoding an antibody from the thus isolated B cells. As examples of the lesion, cancer tissues and so on can be cited. Thus, an antibody gene can be obtained without resort to cloning B cells. As a result, it also becomes possible to obtain a gene encoding a human-origin antibody wherein cloning can be hardly effected. By using a cancer tissue as the lesion, a gene of an antibody against cancer can be obtained.

公开(公告)号：EP1045027A4

公开(公告)日：2002-11-20

申请号：EP99900150

申请日：1999-01-08

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： TSUCHIYA MASAYUKI ,  YOSHIDA KENJI

IPC分类号： C07H21/04 ,  C12N9/12 ,  C12N9/22 ,  C12N15/12 ,  C12Q1/68 ,  C07K16/40 ,  C12P21/02

CPC分类号： C12N9/1276 ,  C12Y207/07049

摘要： A novel gene having a reverse transcriptase motif; the full base sequence of this gene isolated; a protein encoded by the gene; and an antibody against this protein. They are useful in developing a method for detecting telomerase activity, a method for detecting cancer cells, a telomerase activity inhibitor and a method for screening a telomerase activity inhibitor.

摘要翻译： 具有逆转录酶基序的新基因; 该基因的全部碱基序列被分离; 由该基因编码的蛋白质; 和针对这种蛋白质的抗体。 它们可用于开发检测端粒酶活性的方法，检测癌细胞的方法，端粒酶活性抑制剂和筛选端粒酶活性抑制剂的方法。

公开(公告)号：EP2436397A4

公开(公告)日：2014-02-19

申请号：EP10780608

申请日：2010-05-27

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： YOSHIDA KENJI ,  ITO HIROTAKA

IPC分类号： C07K16/22 ,  A61K39/395 ,  A61P35/00

CPC分类号： C07K16/22 ,  A61K45/06 ,  A61K2039/507 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  G01N33/5011 ,  G01N2333/4703 ,  G01N2333/485

摘要： The present inventors identified inhibition of a combination of EGFR ligands that serve as targets for inhibition of cancer cell proliferation. More specifically, EREG antagonists and TGF± antagonists were found to be useful as inhibitors of cell growth. The present invention relates to pharmaceutical compositions containing EGF family ligand antagonists as components.

公开(公告)号：EP1746157A4

公开(公告)日：2009-02-18

申请号：EP05737209

申请日：2005-04-27

申请人： CHUGAI PHARMACEUTICAL CO LTD ,  PHARMALOGICALS RES PTE LTD ,  CENTRAL INST FOR EX ANIMALS IN

发明人： TSUCHIYA MASAYUKI ,  SUZUKI MASAMI ,  YOSHIDA KENJI ,  FUJII ETSUKO ,  WATANABE MIHO ,  MATSUBARA KOICHI ,  CHEN YU JAU ,  SIM JULIANA

IPC分类号： C12N15/09 ,  A01K67/027 ,  A61K31/7088 ,  A61K35/12 ,  A61K35/16 ,  A61K39/395 ,  A61K48/00 ,  A61P31/12 ,  A61P35/00 ,  C07K16/00 ,  C07K16/30 ,  C07K16/32 ,  C12N5/02 ,  C12N5/16 ,  C12P21/08 ,  C12R1/91

CPC分类号： A01K67/027 ,  A01K2267/01 ,  A61K35/16 ,  C07K16/00 ,  C07K16/30

摘要： It is intended to facilitate obtaining of antibody-producing cells infiltrating virus-infected cells, cancerous cells, abnormal cells constituting benign hyperplasia, etc. and to highly efficiently produce an antibody and nucleic acid coding for the same from the antibody-producing cells. It has been found that when a cancer-ridden tissue containing infiltrative lymphocytes is transplanted into a highly immunocompromised animal having none of T cell, B cell and NK cell and exhibiting a low IFN producing capability, unexpectedly, the differentiation and proliferation of infiltrative lymphocytes are promoted, and the number of plasma cells capable of producing an antibody that recognizes the cancer-ridden tissue is strikingly increased, and the plasma cells can be easily isolated, and from the plasma cells, there can be easily prepared the antibody or nucleic acid coding for the antibody.

公开(公告)号：EP1672065A4

公开(公告)日：2007-07-11

申请号：EP04788274

申请日：2004-09-29

申请人： CHUGAI PHARMACEUTICAL CO LTD ,  MATSUSHIMA KOUJI ,  HASHIMOTO SHINICHI

发明人： MATSUSHIMA KOUJI ,  HASHIMOTO SHINICHI ,  TSUCHIYA MASAYUKI ,  HIRATA YUICHI ,  YOSHIDA KENJI ,  OJIMA KAZUYUKI

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K45/00 ,  A61P1/16 ,  A61P11/06 ,  A61P31/12 ,  A61P31/14 ,  A61P35/00 ,  A61P37/04 ,  A61P37/06 ,  A61P37/08 ,  C07K14/705 ,  C07K16/28 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12P21/02 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50 ,  G01N33/564 ,  G01N33/566

CPC分类号： C07K14/705 ,  A61K31/00 ,  G01N33/564 ,  G01N33/566 ,  G01N2500/00

摘要： Purified immunocytes were analyzed for expression frequencies, and the NKIR gene expressed specifically in natural killer (NK) cells was successfully identified. The NKIR gene encodes a receptor. Agonists and antagonists for the receptor can be identified by using the receptor.

公开(公告)号：EP1344831A4

公开(公告)日：2004-06-16

申请号：EP01271868

申请日：2001-12-21

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： SUGO IZUMI ,  YOSHIDA KENJI

IPC分类号： G01N33/50 ,  C12Q1/02 ,  C12N5/10 ,  C12N15/09 ,  G01N33/15 ,  G01N33/48 ,  G01N33/566 ,  G01N33/68

CPC分类号： G01N33/5011 ,  G01N33/5005 ,  G01N2333/924 ,  G01N2510/00

摘要： A method of measuring the cell death of target cells characterized in that target cells containing a gene encoding a membrane protein capable of inducing cell death and a gene encoding a marker protein are incubated and then the marker protein leaked outside the cells due to cell death is measured.