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11 条记录 (耗时 0.038 秒)

    MICROFABRICATED CELL SORTER
    3.
    发明公开
    MICROFABRICATED CELL SORTER 有权
    小型化的细胞分选仪

    公开(公告)号：EP1190229A2

    公开(公告)日：2002-03-27

    申请号：EP99933512.8

    申请日：1999-05-21

    申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

    发明人： QUAKE, Stephen ,  FU, Anne ,  ARNOLD, Frances ,  SPENCE, Charles, F.

    IPC分类号： G01N15/14

    CPC分类号： G01N15/14 ,  G01N2015/149

    摘要： The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.

    MICROFABRICATED CELL SORTER
    6.
    发明授权
    MICROFABRICATED CELL SORTER 有权
    MINIATURISIERTER ZELLSORTIERER

    公开(公告)号：EP1190229B1

    公开(公告)日：2011-10-26

    申请号：EP99933512.8

    申请日：1999-05-21

    申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

    发明人： QUAKE, Stephen ,  FU, Anne ,  ARNOLD, Frances ,  SPENCE, Charles, F.

    IPC分类号： G01N15/14

    CPC分类号： G01N15/14 ,  G01N2015/149

    摘要： The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.

    PRODUCTION OF FUNCTIONAL HYBRID GENES AND PROTEINS
    8.
    发明公开
    PRODUCTION OF FUNCTIONAL HYBRID GENES AND PROTEINS 审中-公开
    生产功能杂合基因和杂合蛋白质

    公开(公告)号：EP1228200A1

    公开(公告)日：2002-08-07

    申请号：EP00975458.1

    申请日：2000-10-27

    申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY ,  Arnold, Frances

    发明人： ARNOLD, Frances ,  SIEBER, Volker ,  ZHANG, Ji, Hu

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/1093 ,  C07K14/43595 ,  C12N9/0071 ,  C12N9/0077 ,  C12N15/1027

    摘要： The invention relates to an improved method for creating gene and protein libraries, particularly random gene libraries encoding for hybrid proteins containing fragments from one or two parent proteins. The method may be used to make libraries of circularly permuted variants of genes encoding a single protein or hybrid proteins, especially single chain proteins. In addition, the invention can be used to make libraries for protein fragment complementation, in which the two fragments originate from one parent protein or from two different parent proteins. The method can produce a library of genes mostly of the correct size, leading to a high fraction of functional hybrids or complements. When coupled with suitable screening or selection, the method can be used to create and identify hybrid proteins, including new proteins with new or altered properties. The invention also provides libraries of hybrid proteins, especially single-chain proteins, that include an N-terminal sequence originating from one parent protein, fused to a C-terminal sequence of a second parent protein, with both sequences varying in length among the hybrids of the library.