NANOPORE SENSING BY LOCAL ELECTRICAL POTENTIAL MEASUREMENT
    3.
    发明公开
    NANOPORE SENSING BY LOCAL ELECTRICAL POTENTIAL MEASUREMENT 审中-公开
    NANOPORENERFASSUNG DURCH LOKALE ELEKTRISCHE POTENZIALMESSUNG

    公开(公告)号:EP2694671A1

    公开(公告)日:2014-02-12

    申请号:EP11720230.9

    申请日:2011-04-29

    IPC分类号: C12Q1/68 G01N33/487 B82Y15/00

    摘要: There is provided a nanopore disposed in a support structure, with a fluidic connection between a first fluidic reservoir and an inlet to the nanopore and a second fluidic connection between a second fluidic reservoir and an outlet from the nanopore. A first ionic solution of a first buffer concentration is disposed in the first reservoir and a second ionic solution of a second buffer concentration, different than the first concentration, is disposed in the second reservoir, with the nanopore providing the sole path of fluidic communication between the first and second reservoirs. An electrical connection is disposed at a location in the nanopore sensor that develops an electrical signal indicative of electrical potential local to at least one site in the nanopore sensor as an object translocates through the nanopore between the two reservoirs.

    摘要翻译: 提供了一种设置在支撑结构中的纳米孔,在第一流体储存器和纳米孔的入口之间具有流体连接,并且在第二流体储存器和来自第一缓冲液浓度的纳米孔第​​一离子溶液的出口之间的第二流体连接 设置在第一储存器中,并且将第二缓冲液浓度与第一浓度不同的第二离子溶液设置在第二储存器中,纳米孔提供第一和第二储存器之间的流体连通的唯一路径。 电连接被设置在纳米孔传感器中的位置处,当物体通过两个储存器之间的纳米孔移位时,该电信号产生指示纳米孔传感器中至少一个位点局部电势的电信号。

    NANOPORE SENSING BY LOCAL ELECTRICAL POTENTIAL MEASUREMENT

    公开(公告)号:EP4109094A1

    公开(公告)日:2022-12-28

    申请号:EP22183113.4

    申请日:2011-04-29

    摘要: There is provided a nanopore disposed in a support structure, with a fluidic connection between a first (cis) fluidic reservoir and an inlet to the nanopore and a second fluidic connection between a second (trans) fluidic reservoir and an outlet from the nanopore; the cis fluidic reservoir having a cis reservoir fluidic access resistance Rcis, or Rc, the trans fluidic reservoir having a trans reservoir fluidic access resistance Rtrans, or Rt, and the nanopore having a nanopore fluidic resistance Rpore, or Rp, wherein Rp is of the same order of magnitude as Rt and both Rp and Rt are at least an order of magnitude greater than Rc. As an embodiment a first ionic solution of a first buffer concentration is disposed in the first reservoir and a second ionic solution of a second buffer concentration, different than the first concentration, is disposed in the second reservoir, with the nanopore providing the sole path of fluidic communication between the first and second reservoirs. An electrical connection is disposed at a location in the nanopore sensor that develops an electrical signal indicative of electrical potential local to at least one site in the nanopore sensor as an object translocates through the nanopore between the two reservoirs.