利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.009 秒)

    METHOD FOR FERMENTING SUGARS USING GENETICALLY ENGINEERED YEAST
    1.
    发明公开
    METHOD FOR FERMENTING SUGARS USING GENETICALLY ENGINEERED YEAST 审中-公开
    使用遗传工程酵母来开发糖的方法

    公开(公告)号：EP3158075A2

    公开(公告)日：2017-04-26

    申请号：EP15733966.4

    申请日：2015-06-18

    申请人： Cargill, Incorporated

    发明人： BRADY, Keith M. ,  MILLER, Christopher K.

    IPC分类号： C12P19/02 ,  C12P7/42 ,  C12P7/56 ,  C12P7/64 ,  C12P7/02 ,  C12P7/40 ,  C12P13/04

    CPC分类号： C12P19/02 ,  C12N15/815 ,  C12P7/02 ,  C12P7/40 ,  C12P7/56 ,  C12P7/6409 ,  C12P7/6436 ,  C12P13/04

    摘要： A fermentation process for fermenting a starch hydrolysate containing 60-98 weight percent of glucose based on total carbohydrate and 0.3-5% weight percent of isomaltose based on total carbohydrate to a non-ethanol fermentation product is carried out. The method comprises: a) forming a fermentation broth containing the starch hydrolysate and a genetically modified microorganism containing i) an exogenous gene encoding transporter capable of transporting isomaltose ii) an exogenous gene encoding an enzyme capable of converting isomaltose to glucose; and b) fermenting the starch hydrolysate in the fermentation broth to produce a non-ethanol fermentation product. This fermentation process may be carried out as a single step fermentation or a batch process. The genetically modified microorganism may contain an exogenous gene encoding an enzyme that catalyzes the formation of a product other than ethanol.

    摘要翻译： 进行用于发酵含有基于总碳水化合物的60-98重量％葡萄糖和基于总碳水化合物的0.3-5重量％异麦芽糖到非乙醇发酵产物的淀粉水解产物的发酵工艺。 该方法包括：a）形成含有淀粉水解产物和经遗传修饰的微生物的发酵液，所述微生物包含i）编码能够运输异麦芽糖的转运蛋白的外源基因ii）编码能够将异麦芽糖转化为葡萄糖的酶的外源基因; 和b）发酵发酵液中的淀粉水解产物以产生非乙醇发酵产物。 该发酵过程可以作为单步发酵或分批过程进行。 经遗传修饰的微生物可含有编码催化除乙醇以外的产物形成的酶的外源基因。