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公开(公告)号：EP2680961B8
公开(公告)日：2018-04-04
申请号：EP12704318.0
申请日：2012-02-01
发明人： BERTHOLLE, Fabien , BIBETTE, Jérôme , BAUDRY, Jean-Marie , BREMOND, Nicolas , BARABAN, Larysa , PANIZZA, Pascal
CPC分类号： C12Q1/025 , A61K6/0023 , B01L3/502784 , B01L2200/0673 , B01L2300/0838 , B01L2300/088 , C12M23/16 , C12M25/01 , G01N35/08 , C08L79/08
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2.发明公开PROCÉDÉ DE CRIBLAGE HAUT DÉBIT DE GOUTTES PAR ÉCHANGE OSMOTIQUE ET VARIATION DE DENSITÉ 审中-公开DROP通过渗透交换和密度变化的高通量筛选方法
公开(公告)号：EP2689012A1
公开(公告)日：2014-01-29
申请号：EP12709345.8
申请日：2012-03-21
申请人： Centre National de la Recherche Scientifique (CNRS) , Université Pierre et Marie Curie (Paris 6) , Ecole Normale Supérieure de Lyon , Université Paris Diderot - Paris 7
发明人： YVERT, Gaël , BIBETTE, Jérôme , BAUDRY, Jean-Marie , BOITARD, Laurent , BREMOND, Nicolas , COTTINET, Denis
IPC分类号： C12N15/00 , C12P1/00 , C12Q1/04 , B01D17/02 , B01D17/04 , G01N15/02 , B03D1/00 , B04B5/04 , G01N15/04 , G01N15/10
CPC分类号： B01D61/002 , B01D19/0036 , B01D61/38 , C12N1/00 , G01N15/0255 , G01N15/04 , G01N2015/1081
摘要： This method comprises bringing drops to be separated into contact with an interface (40) suitable for allowing an osmotic equilibrium between the content of each drop to be separated. The method comprises an osmotic flow between the drops (20A) of the first group of drops through the interface (40) in order to modify the density of each drop (20A) of the first group of drops and the separation of the drops (20A, 20B) according to the density thereof or a combination of the density and the volume in order to isolate the drops (20A) of the first group of drops from the drops (20B) of a second group of drops.
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公开(公告)号：EP3080247A1
公开(公告)日：2016-10-19
申请号：EP14815303.4
申请日：2014-12-12
申请人： Etablissement Français du Sang , Université Pierre et Marie Curie (Paris 6) , Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris , Assistance Publique Hôpitaux de Paris , Centre National de la Recherche Scientifique (C.N.R.S.)
发明人： GIARRATANA, Maria Catalina , DOUAY, Luc , DOMEJEAN, Hugo , BREMOND, Nicolas , BROUCHON, Julie Victoire , BAUDRY, Jean , BIBETTE, Jérôme
IPC分类号： C12N5/078
CPC分类号： C12N5/0012 , C12M25/01 , C12N5/0641 , C12N2500/25 , C12N2501/125 , C12N2501/14 , C12N2501/145 , C12N2501/155 , C12N2501/165 , C12N2501/2303 , C12N2501/2306 , C12N2501/26 , C12N2501/91 , C12N2533/74
摘要： The present invention concerns a capsule comprising at least one cell with blood-forming potential, said capsule being formed of a liquid core and at least one gelled shell completely encapsulating the liquid core at the periphery of same, the use of such a capsule to produce enucleated erythroid cells
ex vivo , as well as an
ex vivo method of producing enucleated erythroid cells using said capsule.-
公开(公告)号：EP2689012B1
公开(公告)日：2019-07-03
申请号：EP12709345.8
申请日：2012-03-21
申请人： Centre National de la Recherche Scientifique (C.N.R.S.) , Université Pierre et Marie Curie (Paris 6) , Ecole Normale Supérieure de Lyon , Université Paris Diderot - Paris 7
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5.发明公开PROCÉDÉ DE RÉCUPÉRATION DE GOUTTES ET SYSTÈME DE RÉCUPÉRATION DE GOUTTES ASSOCIÉ 审中-公开DROP恢复方法及相关DROP恢复系统
公开(公告)号：EP3319728A1
公开(公告)日：2018-05-16
申请号：EP16741562.9
申请日：2016-07-07
申请人： Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris , Centre National de la Recherche Scientifique (CNRS)
摘要： The invention relates to a method for recuperating droplets (4), comprising the following steps: - supplying a chip (20) comprising a fluid circulation duct (46), - injecting an emulsion (6) of droplets, - injecting a carrier fluid (26) to form a working fluid (28) containing droplets of the emulsion which are spaced apart along the duct, - conveying the working fluid through the duct, - injecting a separating fluid (33) in order to separate the working fluid into a plurality of successive pockets (35), each pocket being isolated from the next pocket by a separator (80), - conveying the pockets and separators towards an outlet (66) of the chip, - recuperating in a compartment (82) of a recuperation support (34) at least one pocket containing a droplet recuperated after conveying.
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6.发明公开
公开(公告)号：EP2680961A1
公开(公告)日：2014-01-08
申请号：EP12704318.0
申请日：2012-02-01
发明人： BERTHOLLE, Fabien , BIBETTE, Jérôme , BAUDRY, Jean-Marie , BREMOND, Nicolas , BARABAN, Larysa , PANIZZA, Pascal
CPC分类号： C12Q1/025 , A61K6/0023 , B01L3/502784 , B01L2200/0673 , B01L2300/0838 , B01L2300/088 , C12M23/16 , C12M25/01 , G01N35/08 , C08L79/08
摘要： The invention relates to a method for monitoring a reaction and to a reaction system which are inexpensive, compact, easy to implement, and enable the reaction medium to be fully supervised throughout the entire experiment. For this purpose, the invention relates to a reaction system, in particular for microorganism cultures, including: at least one vessel (M1) for the reaction medium (1, 1'), which is in fluid communication with an injection tube (10); at least one vessel (F1) for a carrier fluid (11) that is immiscible with the reaction medium (1, 1'), which is in fluid communication with a reaction tube (20); the injection tube (10) being mounted so as to lead into the reaction tube (20) such that individual drops (30) of the reaction medium can be injected into the reaction tube (20) and into the immiscible carrier fluid (11), so as to form a train of reaction chambers; at least one detector for monitoring a reaction; a means for classifying the reaction chambers; and at least one means for recirculating reaction chambers in front of at least one detector for monitoring a reaction.
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公开(公告)号：EP2300137A2
公开(公告)日：2011-03-30
申请号：EP09737052.2
申请日：2009-07-13
申请人： Universite Pierre Et Marie Curie - Paris 6 , Centre National de la Recherche Scientifique , Université Paris Diderot - Paris 7
CPC分类号： G01N15/1475 , G01N2015/003 , G01N2015/0092
摘要： The present invention relates to a method for reading an emulsion (3) comprising drops and a continuous phase surrounding the drops, wherein said method comprises: two-dimensional scanning of the emulsion (3), and construction of a two-dimensional image of the emulsion (3) on the basis of said scanning. Preferably, the drops do not move during the scanning, this being achieved, for example, by solidifying the continuous phase or by using a compact or semi-compact two-dimensional network of drops. The method according to the invention may also comprise monitoring over time a chemical or biological reaction taking place in at least one of the drops. The invention also relates to a device which implements this method. Use in the detection and/or sorting of microdrops playing the role of microreactors or containing specific cells or molecules, in fields such as gene expression or diagnosis.
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8.发明公开METHOD FOR MANIPULATING THE EVOLUTION OF COLLECTIVES OF SELF-REPLICATING ENTITIES AND VARIATION BETWEEN COLLECTIVES OF SELF-REPLICATING ENTITIES 审中-公开进行操纵的开发方法和差异之间的实体自我复制自我复制的实体社区
公开(公告)号：EP3103862A1
公开(公告)日：2016-12-14
申请号：EP15305891.2
申请日：2015-06-11
申请人： Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris , Centre National de la Recherche Scientifique (C.N.R.S.) , Ecole Normale Supérieure
发明人： RAINEY, Paul , BIBETTE, Jérôme , BAUDRY, Jean , BREMOND, Nicolas , BOITARD, Laurent , GARNICA, Jairo , COTTINET, Denis
摘要： The present invention relates to a method for manipulating the evolution of collectives of self-replicating entities and/or variation between collectives of self-replicating entities, in a high throughput droplet milli-fluidic system.
摘要翻译： 本发明涉及一种用于操纵自我复制的实体和/或自我复制实体的集体之间的变化的集体的演变，以高通量液滴毫流体系统。
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公开(公告)号：EP4075118A1
公开(公告)日：2022-10-19
申请号：EP22172592.2
申请日：2009-07-13
申请人： SORBONNE UNIVERSITE , Centre National de la Recherche Scientifique (C.N.R.S.) , Université Paris Cité , Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris
IPC分类号： G01N15/14
摘要： La présente invention concerne un procédé de lecture d'une émulsion (3) comprenant des gouttes et une phase continue entourant les gouttes, ledit procédé comprenant :
- un balayage bidimensionnel de l'émulsion (3), et
- une construction d'une image bidimensionnelle de l'émulsion (3) à partir dudit balayage.
De préférence, les gouttes ne bougent pas pendant le balayage, par exemple en solidifiant la phase continue ou en utilisant un réseau bidimensionnel compact ou semi compact de gouttes. Le procédé selon l'invention peut comprendre en outre un suivi dans le temps d'une réaction chimique ou biologique se déroulant dans au moins une des gouttes.
L'invention concerne aussi un dispositif mettant en œuvre ce procédé.
Application à la détection et/ou le tri de microgouttes jouant le rôle de microréacteurs ou contenant des cellules ou des molécules spécifiques, dans des domaines tels que l'expression de gènes ou le diagnostic.-
公开(公告)号：EP3319728B1
公开(公告)日：2020-03-18
申请号：EP16741562.9
申请日：2016-07-07
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