公开(公告)号：EP2350617A2

公开(公告)日：2011-08-03

申请号：EP09744320.4

申请日：2009-10-21

申请人： ChemoMetec A/S

发明人： KJÆRULFF, Søren ,  SKINDERSØ, Mette Elena ,  SØRENSEN, Helle Frobøse ,  RAVN, Frans ,  GLENSBJERG, Martin

IPC分类号： G01N21/64 ,  C12N15/00 ,  G01N33/50 ,  G02B21/06 ,  G02B27/09 ,  G01N1/30 ,  G01N33/58

CPC分类号： G01N21/64 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/0631 ,  G02B27/0905 ,  G02B27/0961

摘要： According to an embodiment of the invention, an apparatus to detect fluorescence from a sample is disclosed. The apparatus comprises a sample plane onto which the sample is arranged, an excitation light unit including at least a light source to illuminate the sample, and a detection unit comprising at least a detector having at least 100,000 active detection elements to detect a fluorescence signal from the sample.

公开(公告)号：EP2312943B1

公开(公告)日：2019-11-06

申请号：EP09776240.5

申请日：2009-07-13

申请人： Chemometec A/S

发明人： SKINDERSØ, Mette Elena ,  SØRENSEN, Helle Frobøse ,  KJÆRULFF, Søren

IPC分类号： A01N1/02 ,  G01N15/14 ,  G01N21/64 ,  G01N33/50 ,  C09B15/00 ,  C09B57/00 ,  C09B57/02 ,  G01N33/58

公开(公告)号：EP2312943A2

公开(公告)日：2011-04-27

申请号：EP09776240.5

申请日：2009-07-13

申请人： Chemometec A/S

发明人： SKINDERSØ, Mette Elena ,  SØRENSEN, Helle Frobøse ,  KJÆRULFF, Søren

IPC分类号： A01N1/02 ,  G01N15/14 ,  G01N21/64 ,  G01N33/50

CPC分类号： G01N33/582 ,  C09B15/00 ,  C09B57/00 ,  C09B57/02 ,  G01N21/6428 ,  G01N33/502 ,  G01N33/583 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2510/00

摘要： The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).

公开(公告)号：EP2350617B1

公开(公告)日：2018-01-10

申请号：EP09744320.4

申请日：2009-10-21

申请人： ChemoMetec A/S

发明人： KJÆRULFF, Søren ,  SKINDERSØ, Mette Elena ,  SØRENSEN, Helle Frobøse ,  RAVN, Frans ,  GLENSBJERG, Martin

IPC分类号： G01N21/64 ,  C12N15/00 ,  G01N33/50 ,  G02B21/06 ,  G02B27/09 ,  G01N1/30 ,  G01N33/58

CPC分类号： G01N21/64 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/0631 ,  G02B27/0905 ,  G02B27/0961

摘要： According to an embodiment of the invention, an apparatus to detect fluorescence from a sample is disclosed. The apparatus comprises a sample plane onto which the sample is arranged, an excitation light unit including at least a light source to illuminate the sample, and a detection unit comprising at least a detector having at least 100,000 active detection elements to detect a fluorescence signal from the sample.