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    METHOD AND KIT FOR ASSESSING VIABLE CELLS
    3.
    发明公开
    METHOD AND KIT FOR ASSESSING VIABLE CELLS 审中-公开
    方法和试剂盒FOR法官活菌

    公开(公告)号：EP2312943A2

    公开(公告)日：2011-04-27

    申请号：EP09776240.5

    申请日：2009-07-13

    申请人： Chemometec A/S

    发明人： SKINDERSØ, Mette Elena ,  SØRENSEN, Helle Frobøse ,  KJÆRULFF, Søren

    IPC分类号： A01N1/02 ,  G01N15/14 ,  G01N21/64 ,  G01N33/50

    CPC分类号： G01N33/582 ,  C09B15/00 ,  C09B57/00 ,  C09B57/02 ,  G01N21/6428 ,  G01N33/502 ,  G01N33/583 ,  G01N2021/6439 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2510/00

    摘要： The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).

    IMAGE FORMING CYTOMETER
    9.
    发明公开
    IMAGE FORMING CYTOMETER 审中-公开
    图像形成细胞仪

    公开(公告)号：EP3004838A1

    公开(公告)日：2016-04-13

    申请号：EP14733071.6

    申请日：2014-05-28

    申请人： ChemoMetec A/S

    发明人： GLENSBJERG, Martin ,  HOLM, Johan ,  KJÆRULFF, Søren ,  RAVN HANSEN, Frans Ejner

    IPC分类号： G01N15/14

    CPC分类号： G01N15/1434 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/144 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024

    摘要： The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field, dark field or one or more sources of excitation light. The system comprises illumination means (11, 112), a sample holder (100), a sample compartment (101), imaging means (120), collection means (121), light modulation means (122, 123), and detection means (130) with active detection elements (131).

    摘要翻译： 本发明涉及典型地在低光学放大率下进行图像细胞术分析的方法和系统，其中分析基于使用UV明场，暗场或一个或多个激发光源的生物粒子的检测。 该系统包括照明装置（11,112），样品架（100），样品室（101），成像装置（120），收集装置（121），光调制装置（122,123）和检测装置（ 130）与有源检测元件（131）。

    METHOD FOR ANALYSIS OF CELLULAR DNA CONTENT
    10.
    发明公开
    METHOD FOR ANALYSIS OF CELLULAR DNA CONTENT 审中-公开
    VERFAHREN ZUR ANALYZE VONZELLULÄREMDNA-GEHALT

    公开(公告)号：EP2534463A1

    公开(公告)日：2012-12-19

    申请号：EP11705805.7

    申请日：2011-02-10

    申请人： ChemoMetec A/S

    发明人： KJÆRULFF, Søren ,  GLENSBJERG, Martin

    IPC分类号： G01N1/30 ,  C12Q1/68 ,  G01N15/14

    摘要： Disclosed is a fast and simple method for quantification of nucleic acid of biological cells as 2-step protocol. In the first step cells are treated with an acidic solution containing a non-ionic detergent and a fluorescent DNA specific label. In the second step the sample may be neutralized. Determining of the content of nucleic can be performed by fluorescence microscopy. The method may also be used for obtaining information of cell cycle analysis, ploidy determination, measurements of nucleotide incorporation and assays for proliferation, health, stress level, apoptosis, necrosis, or other state of conditions of cells. The invention also relates to a kit of parts comprising an acidic agent, a detergent, a labelling agent and optionally a neutralization agent.

    摘要翻译： 公开了一种用于定量生物细胞核酸的快速和简单的方法，作为2步方案。 在第一步中，用含有非离子洗涤剂和荧光DNA特异性标记的酸性溶液处理细胞。 在第二步中，样品可能被中和。 核酸含量的测定可以通过荧光显微镜进行。 该方法还可用于获得细胞周期分析，倍性测定，核苷酸掺入测定和增殖测定，健康，应激水平，凋亡，坏死或其他细胞状态的信息。 本发明还涉及包含酸性试剂，洗涤剂，标记试剂和任选的中和剂的试剂盒。