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    SEPARATION OF CHARGED PARTICLES BY A SPATIALLY AND TEMPORALLY VARYING ELECTRIC FIELD
    4.
    发明公开
    SEPARATION OF CHARGED PARTICLES BY A SPATIALLY AND TEMPORALLY VARYING ELECTRIC FIELD 失效
    的带电粒子的通过空间和时间上变化的电场分离

    公开(公告)号：EP0972187A1

    公开(公告)日：2000-01-19

    申请号：EP97917713.6

    申请日：1997-03-26

    申请人： Curagen Corporation

    发明人： BADER, Joel, S. ,  ROTHBERG, Jonathan, M. ,  DEEM, Michael, W. ,  MULHERN, Gregory, T. ,  WENT, Gregory, T.

    IPC分类号： G01N27/26 ,  G01N27/447

    CPC分类号： B82Y30/00 ,  G01N27/44773

    摘要： A device for separating charged particles comprising: an upper substate (11); a lower substrate (12); separation lanes (15) defined on the upper substrate, a first plurality of electrodes (21, 22...); a first pad (18), said first plurality of electrodes connected to said first pad (13); a second plurality of electrodes (21, 23...); a second pad (14), said second plurality of electrodes connected to said second pad; said pads and electrodes deposited on the lower substrate; and said first plurality of electrodes and said second plurality of electrodes are interdigitated. During the operation of the device, charged particles are subjected to an electric potential that is cycled between an off-state and one or more on states, in which the potential is preferably spatially periodic with a plurality of eccentrically shaped stationary potential wells.

    NUCLEIC ACID PROBE ARRAYS
    6.
    发明公开
    NUCLEIC ACID PROBE ARRAYS 审中-公开
    NUCLEIC探针阵列

    公开(公告)号：EP1234058A2

    公开(公告)日：2002-08-28

    申请号：EP00980700.9

    申请日：2000-11-27

    申请人： Curagen Corporation

    发明人： ROTHBERG, Jonathan, M. ,  BADER, Joel, S.

    IPC分类号： C12Q1/68

    CPC分类号： B82Y30/00 ,  C12Q1/682 ,  C12Q1/6853 ,  C12Q2600/156 ,  C12Q2565/519 ,  C12Q2531/125 ,  C12Q2521/501

    摘要： Disclosed are nucleic acid probe arrays and methods of identifying and sequencing nucleic acids in a population of nucleic acids using the arrays. The method is preferably performed by annealing a nucleic acid template to an anchor primer attached to a durface of the array. At least one anchor in the array has a sequence complementary to sequences at the 5' and 3' termini of a target nucleic acid.The annealed linear target nucleic acid is circularized using one or two ligation reactions. In one embodiment, one liggation is issued. Annealing of of the linear nucleic acid results in juxtaposition of the 5' and 3' termini of the target nucleic acid on the anchor primer. Addition of a ligase results in circulization of the target nucleic acid. This circularized nucleic acid is a template for extension of the anchor primer in a rolling circle amplification reaction. An extended anchor primer containing multiple copies of a sequence complementary to the circular mucleic acid is also referred to herein as a anchor primer nucleid acid-nucleic acid concatamer. The presence of multiple copies of the complementary sequence facilitates detection of the nucleic acid. Thus, the method provides for a highly sensitive method of detecting a desired nucleic acid attached at a discrete location on the array.

    METHOD AND APPARATUS FOR DISTANCE MEASUREMENTS WITH SOLID-STATE NMR
    7.
    发明公开
    METHOD AND APPARATUS FOR DISTANCE MEASUREMENTS WITH SOLID-STATE NMR 失效
    方法和装置固体中距离测量NMR

    公开(公告)号：EP0990141A1

    公开(公告)日：2000-04-05

    申请号：EP97925527.0

    申请日：1997-05-12

    申请人： Curagen Corporation

    发明人： JARVIE, Thomas, P. ,  BADER, Joel, S. ,  WENT, Gregory, T.

    IPC分类号： G01N24/12 ,  G01N33/53 ,  G06F19/00 ,  G06F159/00

    CPC分类号： G01R33/4625 ,  G01R33/4641 ,  Y10T436/24

    摘要： This invention includes methods for analyzing data generated by various solid-state NMR experiments, including rotational echo double resonance (REDOR), transferred echo double resonance (TEDOR), dipolar recoupling at the magic angle (DRAMA), dipolar recoupling with a windowless sequence (DRAWS), and melding of spin-locking and DRAMA (MELODRAMA). The methods are based alternately on a new analytical transform or the maximum entropy method and their multidimensional extensions. They permit simultaneous, multiple distance measurements of high accuracy and precision, even from nuclei with identical chemical shifts. By providing high quality easily obtained distance measurement from disordered solid state materials, this invention also improves drug discovery and design through fast determination of structures of pharmaceutical lead compounds, drug molecules, or their targets.

    SEPARATION OF CHARGED PARTICLES BY A SPATIALLY AND TEMPORALLY VARYING ELECTRIC FIELD
    10.
    发明公开
    SEPARATION OF CHARGED PARTICLES BY A SPATIALLY AND TEMPORALLY VARYING ELECTRIC FIELD 审中-公开
    的带电粒子的通过空间和时间上变化的电场分离

    公开(公告)号：EP1147409A1

    公开(公告)日：2001-10-24

    申请号：EP99966079.8

    申请日：1999-12-09

    申请人： Curagen Corporation

    发明人： BADER, Joel, S. ,  ROTHBERG, Jonathan, M. ,  DEEM, Michael, W. ,  MULHERN, Gregory, T. ,  WENT, Gregory, T. ,  SIMPSON, John ,  HENCK, Steven

    IPC分类号： G01N27/26 ,  G01N27/447

    CPC分类号： B82Y30/00 ,  G01N27/44773

    摘要： This invention relates to a method and device for separating particles according to their diffusivities in a separation medium by means of a spatially and temporally varying electric potential. The method takes advantage of the transport of charged particles subject to an electric potential that is cycled between an off-state (in which the potential is flat) and one or more on-states, in which the potential is preferably spatially periodic with a plurality of eccentrically shaped stationary wells. The potential wells are at a constant spatial positions in the on-state. Differences in liquid-phase diffusivities lead to charged particle separation. A separation medium fills physically defined separation lanes (15) in the device. Electrodes deposited substantially transverse to the lanes create the required potential. Advantageously, injection ports (16) allow sample loading, and special gating electrodes focus the sample prior to separation.