公开(公告)号：EP2525650A2

公开(公告)日：2012-11-28

申请号：EP11735271.6

申请日：2011-01-21

申请人： Dow AgroSciences, LLC

发明人： AINLEY, William, Michael ,  BLUE, Ryan, C. ,  MURRAY, Michael, G. ,  CORBIN, David, Richard ,  MILES, Rebecca, Ruth ,  WEBB, Steven, R.

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/29

CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

公开(公告)号：EP2893023B1

公开(公告)日：2018-08-15

申请号：EP13834887.5

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： COGAN, Noel ,  FORSTER, John ,  HAYDEN, Matthew ,  SAWBRIDGE, Tim ,  SPANGENBERG, German ,  WEBB, Steven, R. ,  GUPTA, Manju ,  AINLEY, William, Michael ,  HENRY, Matthew, J. ,  MILLER, Jeffrey, C. ,  GUSCHIN, Dmitry, Y.

IPC分类号： C12N15/10 ,  C12N15/82 ,  C12N15/90 ,  C12N5/04 ,  C12N9/22 ,  A01H1/06 ,  A01H5/00

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：EP2525650B1

公开(公告)日：2017-03-15

申请号：EP11735271.6

申请日：2011-01-21

申请人： Dow AgroSciences, LLC

发明人： AINLEY, William, Michael ,  BLUE, Ryan, C. ,  MURRAY, Michael, G. ,  CORBIN, David, Richard ,  MILES, Rebecca, Ruth ,  WEBB, Steven, R.

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/29

CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

公开(公告)号：EP2820136B1

公开(公告)日：2018-03-28

申请号：EP13708634.4

申请日：2013-02-28

申请人： Dow AgroSciences LLC

发明人： OWENS MERLO, Patricia, Ann ,  LARSEN, Cory ,  BEVAN, Scott, A. ,  DAVIES, John, P. ,  REDDY, Vaka, S. ,  AINLEY, William, Michael ,  THOMPSON, Mark, Allen

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N15/8234

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

公开(公告)号：EP2820136A1

公开(公告)日：2015-01-07

申请号：EP13708634.4

申请日：2013-02-28

申请人： Dow AgroSciences LLC

发明人： OWENS MERLO, Patricia, Ann ,  LARSEN, Cory ,  BEVAN, Scott, A. ,  DAVIES, John, P. ,  REDDY, Vaka, S. ,  AINLEY, William, Michael ,  THOMPSON, Mark, Allen

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N15/8234

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

公开(公告)号：EP1809736A2

公开(公告)日：2007-07-25

申请号：EP05851384.7

申请日：2005-11-04

申请人： Dow AgroSciences LLC

发明人： AINLEY, William, Michael ,  BERINGER, Jeffrey, R. ,  LARSEN, Cory, Michelle, Leatherman ,  LU, Min ,  SHEN, Liu, Yin ,  JAYAKUMAR, Pon, Samuel ,  GARRISON, Robbi, Janette ,  PAREDDY, Dayakar, Reddy

IPC分类号： C12N5/02 ,  C12N5/14 ,  C12N5/04

CPC分类号： A01N3/00 ,  A01N1/02 ,  A01N1/0278 ,  C12N5/04

摘要： The present invention relates to methods for the cryopreservation of transformed and non-transformed cells. Also provided by the subject invention are methods of recovering cells that have been cryopreserved. Cultures of cells that have been successfully recovered from cryopreservation are also provided.

公开(公告)号：EP2893023A2

公开(公告)日：2015-07-15

申请号：EP13834887.5

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： COGAN, Noel ,  FORSTER, John ,  HAYDEN, Matthew ,  SAWBRIDGE, Tim ,  SPANGENBERG, German ,  WEBB, Steven, R. ,  GUPTA, Manju ,  AINLEY, William, Michael ,  HENRY, Matthew, J. ,  MILLER, Jeffrey, C. ,  GUSCHIN, Dmitry, Y.

IPC分类号： C12N15/66

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

摘要翻译： 通过以定点方式切割FAD2基因在细胞中的位置来在FAD2基因座中进行基因编辑或基因堆叠以在FAD2基因中产生断裂，然后将与之相关的核酸分子 披露了一个或多个感兴趣的特征。

公开(公告)号：EP2118269B1

公开(公告)日：2013-03-13

申请号：EP07869981.6

申请日：2007-12-27

申请人： Dow AgroSciences LLC

发明人： JAYAKUMAR, Pon, Samuel ,  BERINGER, Jeffrey, R. ,  SCHMITZER, Paul ,  BURROUGHS, Frank ,  GARISSON, Robbi ,  AINLEY, William, Michael ,  SAMBOJU, Narasimha, Chary

IPC分类号： C12N5/00 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12N15/8201

公开(公告)号：EP2118269A2

公开(公告)日：2009-11-18

申请号：EP07869981.6

申请日：2007-12-27

申请人： Dow AgroSciences LLC

发明人： JAYAKUMAR, Pon, Samuel ,  BERINGER, Jeffrey, R. ,  SCHMITZER, Paul ,  BURROUGHS, Frank ,  GARISSON, Robbi ,  AINLEY, William, Michael ,  SAMBOJU, Narasimha, Chary

IPC分类号： C12N5/00 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12N15/8201

摘要： The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin- degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker- free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

公开(公告)号：EP1848806A1

公开(公告)日：2007-10-31

申请号：EP06701862.2

申请日：2006-02-06

申请人： Dow AgroSciences LLC ,  Plantigen Inc.

发明人： JEVNIKAR, Anthony ,  MA, Shengwu ,  AINLEY, William, Michael ,  MERLO, Donald Joseph ,  RUSSELL, Sean, Michael ,  ARMSTRONG, Janna, M.

IPC分类号： C12N15/82 ,  C12N15/60 ,  C12N15/24 ,  A61P3/10 ,  A61K31/52 ,  A61K31/519 ,  A61K39/395 ,  A61K39/00 ,  A61K38/20 ,  A61K38/13

CPC分类号： A61K31/52 ,  A61K31/519 ,  A61K38/00 ,  A61K38/13 ,  A61K38/2066 ,  A61K38/28 ,  A61K39/39541 ,  A61K45/06 ,  A61K2039/505 ,  C07K14/5406 ,  C07K16/2809 ,  C07K16/40 ,  C07K2319/04 ,  C07K2319/21 ,  C12N9/88 ,  C12N15/8257 ,  C12N15/8258 ,  A61K2300/00 ,  A61K38/51

摘要： The invention is directed to a new method for the treatment of new onset Type I diabetes in mammals or for the treatment of pre-Type I diabetic mammals where the method comprises administering (a) anti-T cell therapy to the mammal and administering (b) an autoantigen and optional mucosal antigen composition, wherein (a) and (b) are administered concurrently or sequentially Exemplified is a treatment using a mixture of anti-CD3 antibodies, a glutamic acid decarboxylase (GAD) autoantigen, and an immunoregulatory cytokine Canme GAD sequences are also disclosed.