公开(公告)号：EP3978513A1

公开(公告)日：2022-04-06

申请号：EP20815305.6

申请日：2020-05-29

申请人： Editforce, Inc. ,  Kyushu University, National University Corporation

发明人： YAGI,Yusuke ,  NAKAMURA, Takahiro

IPC分类号： C07K14/00 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/11 ,  C12N15/63

摘要： A PPR protein with high performance is provided. A PPR protein that binds to a long nucleotide sequence is provided by linking motifs in a number larger than conventionally used 7 to 14. A PPR motif is provided, of which typical examples are the followings: (A-1) a PPR motif consisting of the sequence of SEQ ID NO: 9 or 401, (C-1) a PPR motif consisting of the sequence of SEQ ID NO: 10, (G-1) a PPR motif consisting of the sequence of SEQ ID NO: 11, and (U-1) a PPR motif consisting of the sequence of SEQ ID NO: 12. These motifs are useful as PPR motifs for adenine, cytosine, guanine, and uracil in a target nucleotide sequence, respectively.

公开(公告)号：EP4242237A1

公开(公告)日：2023-09-13

申请号：EP21889216.4

申请日：2021-11-04

申请人： Editforce, Inc. ,  Kyushu University, National University Corporation

发明人： YAGI, Yusuke ,  OHTA, Masaru ,  NAKAMURA, Takahiro

IPC分类号： C07K19/00 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/55 ,  C12N15/62 ,  C12N9/16

摘要： It was found that four nuclease domain mutants have nuclease activity superior to that of a wild-type nuclease domain and are capable of enhancing genome editing efficiency in combination with various nucleic acid binding domains.

公开(公告)号：EP3977849A1

公开(公告)日：2022-04-06

申请号：EP20812826.4

申请日：2020-05-29

申请人： Editforce, Inc. ,  Kyushu University, National University Corporation

发明人： YAGI, Yusuke ,  IMAI, Takayoshi ,  TAMAI, Takayuki ,  NAKAMURA, Takahiro ,  TERAMOTO, Takamasa

IPC分类号： A01K67/027 ,  C07K7/06 ,  C07K7/08 ,  C07K14/46 ,  C07K19/00 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/10 ,  C12N15/12 ,  C12N15/63 ,  C12Q1/68

摘要： In order to improve aggregation property of a PPR protein, the A 6 amino acid of the 1st PPR motif from the N-terminus (M 1 ) is made more hydrophilic. Further, the A 9 amino acid of M 1 is made to be a hydrophilic amino acid or glycine. The A 6 amino acid is preferably asparagine or aspartic acid, and the A 9 amino acid is preferably glutamine, glutamic acid, lysine, or glycine. Proteins containing such a PPR motif as M 1 motif may have not only improved aggregation property, but also high binding power to a target nucleic acid.

公开(公告)号：EP2784157A1

公开(公告)日：2014-10-01

申请号：EP12841435.6

申请日：2012-10-22

申请人： Kyushu University, National University Corporation

发明人： NAKAMURA, Takahiro ,  YAGI, Yusuke ,  KOBAYASHI, Keiko

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07K14/00

CPC分类号： G16B30/00 ,  C07K14/415 ,  C07K2319/85 ,  C12N15/8216 ,  C12N15/8287 ,  C12N15/8289 ,  C12Q1/68 ,  C12Q2522/101 ,  G01N33/5308 ,  G16B15/00 ,  G16B20/00 ,  G16B35/00 ,  G16C20/60

摘要： A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an α-helix structure, and is represented by the formula 2, wherein, in the formula 2, A 1 to A 12 independently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an α-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered "i" (-1), "ii" (-2), and so on from the C-terminus side, provided that L iii to L vii may not exist), and combination of three amino acids A 1 , A 4 and L ii , or combination of two amino acids A 4 , and L ii is a combination corresponding to a target RNA base or base sequence.

摘要翻译： 提供了以RNA碱基选择性方式或RNA碱基序列特异性结合的蛋白质的设计方法。 本发明的蛋白质是含有一个或多个PPR基序（优选2至14个PPR基序）的蛋白质，每个PPR基序由由式1表示的30至38个氨基酸长度的多肽组成（其中螺旋A是部分 能够形成± - 螺旋结构的12个氨基酸长度，并且由式2表示，其中在式2中，A 1至A 12独立地表示氨基酸; X不存在或为部分 1至9个氨基酸长度;螺旋B是能形成± - 螺旋结构的11至13个氨基酸长度的部分; L是2-至7-氨基酸长度的部分，由 式3，其中，在式3中，如果L iii至L vii不可以，则从C末端侧的氨基酸编号为“i”（-1），“ii”（-2）等等 存在），并且三个氨基酸A 1，A 4和L ii的组合，或两个氨基酸A 4和L ii的组合是对应于靶RNA碱基或碱基序列的组合。

公开(公告)号：EP3466978A1

公开(公告)日：2019-04-10

申请号：EP17806675.9

申请日：2017-05-30

申请人： Kyushu University, National University Corporation

发明人： NAKAMURA, Takahiro ,  YAGI, Yusuke

IPC分类号： C07K19/00 ,  C07K14/415 ,  C07K14/435 ,  C12N15/09

摘要： [Problem to be Solved] The object of the present invention is to develop a method of regulating a target RNA.
[Solution] There is provided a fusion protein comprising a functional domain which improves the protein expression level from mRNA and a PPR protein which can bind to a target mRNA in an RNA base-selective or RNA base sequence-specific manner.

公开(公告)号：EP4130274A1

公开(公告)日：2023-02-08

申请号：EP21781411.0

申请日：2021-03-31

申请人： Editforce, Inc.

发明人： ICHINOSE, Mizuho ,  GUTMANN, Bernard ,  YAGI, Yusuke ,  AKAIWA, Yumi ,  SHIMAJIRI, Yasuka ,  NAKAMURA, Takahiro

IPC分类号： C12N15/52 ,  C07K14/415 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/29

摘要： A method for converting an editing target C contained in a target RNA to U or an editing target U contained in a target RNA to C is provided. A method for editing a target RNA, which comprises applying an artificial DYW protein containing a DYW domain consisting of any one of the polypeptides a, b, c, and be mentioned below to the target RNA: a. a polypeptide having x a1 PGx a2 SWIEx a3 -x a16 HP ... Hx aa E ... Cx a17 x a18 CH ... DYW, having a sequence identity of at least 40% to the sequence of SEQ ID NO: 1, and having a C-to-U/U-to-C editing activity, b. a polypeptide having x b1 PGx b2 SWWTDx b3 -x b16 HP ... Hx bb E ... Cx b17 x b18 CH ... DYW, having a sequence identity of at least 40% to the sequence of SEQ ID NO: 2, and having a C-to-U/U-to-C editing activity, c. a polypeptide having KPAx c1 Ax c2 IEx c3 ... Hx cc E ... Cx c4 x c5 CH ... x c6 x c7 x c8 , having a sequence identity of at least 40% to the sequence of SEQ ID NO: 3, and having a C-to-U/U-to-C editing activity, and be. a polypeptide having x b1 PGx b2 SWWTDx b3 -x b16 HP ... Hx cc E ... Cx c4 x c5 CH ... Dx bc1 x bc2 , having a sequence identity of at least 40% to the sequence of SEQ ID NO: 90, and having a C-to-U/U-to-C editing activity (in the sequences, x represents an arbitrary amino acid, and ... represents an arbitrary polypeptide fragment).