公开(公告)号：EP2694536A2

公开(公告)日：2014-02-12

申请号：EP12710298.6

申请日：2012-03-27

申请人： Evonik Degussa GmbH

发明人： HANS, Stephan ,  BATHE, Brigitte ,  RETH, Alexander ,  CLAES, Wilfried ,  KRÄMER, Reinhard ,  SEIBOLD, Gerd ,  HENRICH, Alexander

IPC分类号： C07K14/34 ,  C12P13/08 ,  C12N1/21

CPC分类号： C07K14/34 ,  C12P13/08

摘要： The invention relates to a microorganism which produces and/or secretes an organo-chemical compound, the microorganism demonstrating an expression of one or more protein subunits of the ABC transporter with the activity of a trehalose importer, which expression is increased in comparison with the respective starting strain, where the microorganism is capable of taking up trehalose from the medium; and to a process for the production of an organo-chemical compound using the microorganism according to the invention, where an accumulation of trehalose in the fermentation broth is reduced or avoided.

公开(公告)号：EP2446039A1

公开(公告)日：2012-05-02

申请号：EP10725186.0

申请日：2010-06-18

申请人： Evonik Degussa GmbH

发明人： WENDISCH, Volker, F. ,  LINDNER, Steffen ,  BATHE, Brigitte ,  CLAES, Wilfried

IPC分类号： C12N15/52 ,  C12N9/12 ,  C12N15/63 ,  C12N15/77 ,  C12N1/21 ,  C12P13/08 ,  C12R1/15 ,  C12R1/19

CPC分类号： C12P13/08 ,  C12N9/1205 ,  C12Y207/01023

摘要： The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.

摘要翻译： 本发明涉及一种制备有机化合物的方法，其特征在于进行以下步骤：a）分泌L-氨基酸的微生物的发酵，该微生物含有编码多磷酸依赖性多肽的过表达多核苷酸 NAD +激酶活性，在发酵培养基中形成发酵液，b）所述化合物在所述发酵液中和/或所述微生物细胞中的积累。 本发明涉及通过发酵其中具有多磷酸依赖性NAD +激酶的多肽过表达的微生物来制备有机化合物的方法。

公开(公告)号：EP2446039B1

公开(公告)日：2018-05-02

申请号：EP10725186.0

申请日：2010-06-18

申请人： Evonik Degussa GmbH

发明人： WENDISCH, Volker, F. ,  LINDNER, Steffen ,  BATHE, Brigitte ,  CLAES, Wilfried

IPC分类号： C12N15/52 ,  C12N9/12 ,  C12N15/63 ,  C12N15/77 ,  C12N1/21 ,  C12P13/08 ,  C12R1/15 ,  C12R1/19

CPC分类号： C12P13/08 ,  C12N9/1205 ,  C12Y207/01023

摘要： The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.

公开(公告)号：EP2951310A1

公开(公告)日：2015-12-09

申请号：EP14700603.5

申请日：2014-01-13

申请人： Evonik Degussa GmbH

发明人： WALTER, Frederik ,  PERSICKE, Marcus ,  KALINOWSKI, Jörn ,  HÜSER, Andrea ,  CLAES, Wilfried ,  RETH, Alexander

IPC分类号： C12P13/08 ,  C12P13/12 ,  C12N9/02

CPC分类号： C12R1/15 ,  C12N9/0036 ,  C12N9/0051 ,  C12P7/40 ,  C12P13/001 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/227 ,  C12P13/24 ,  C12P19/30 ,  C12P19/38

摘要： The invention relates to a microorganism which produces and/or secretes an organic chemical compound, wherein the microorganism has an increased expression, in comparison with the respective starting strain, of a polypeptide LpdA having the activity of a transhydrogenase; and also a method for producing an organic chemical compound, using the microorganism according to the invention.

摘要翻译： 使用微生物发酵有机化合物的方法包括：（a）产生有机化合物的微生物的发酵，其中微生物在编码多肽的多核苷酸中过表达，所述多核苷酸的氨基酸序列与氨基酸具有至少80％的同一性 SEQ ID NO：2的酸序列，（b）从（a）的发酵液中富集有机化合物，其中未定义的序列可以在ftp://ftp.wipo.int/pub/ 出版-PCT-序列/出版物。

公开(公告)号：EP2553113A2

公开(公告)日：2013-02-06

申请号：EP11710196.4

申请日：2011-03-24

申请人： Evonik Degussa GmbH

发明人： CLAES, Wilfried ,  GERSTMEIR, Robert

IPC分类号： C12P13/10

CPC分类号： C12N15/77 ,  C12P13/10

摘要： The invention relates to a method for the production of L-ornithine by fermentation using microorganisms, which is characterized by an increased export of the amino acid.

摘要翻译： 一种用于通过在增加的氨基酸的出口为特征的L-鸟氨酸使用微生物的发酵制备方法。

公开(公告)号：EP2041276B1

公开(公告)日：2012-02-29

申请号：EP07786778.6

申请日：2007-06-20

申请人： Evonik Degussa GmbH

发明人： BATHE, Brigitte ,  CLAES, Wilfried

IPC分类号： C12N9/12

CPC分类号： C12N9/88 ,  C12N9/1217 ,  C12P13/06 ,  C12P13/08 ,  C12Y203/03001 ,  C12Y207/02004

摘要： The invention relates to novel polynucleotides, coding for a polypeptide with citrate synthase activity, bacteria comprising the same, preferably of the species Corynebacterium and Escherichia, and methods for producing amino acids using said bacteria. The invention particularly relates to an isolated polynucleotide, coding for a polypeptide with the amino acid sequence SEQ ID NO:2, wherein the L-aspartic acid in position 5 of the amino acid sequence is replaced by another proteinogenic amino acid and the polypeptide has citrate synthase activity.

公开(公告)号：EP2694536B1

公开(公告)日：2018-07-25

申请号：EP12710298.6

申请日：2012-03-27

申请人： Evonik Degussa GmbH

发明人： HANS, Stephan ,  BATHE, Brigitte ,  RETH, Alexander ,  CLAES, Wilfried ,  KRÄMER, Reinhard ,  SEIBOLD, Gerd ,  HENRICH, Alexander

IPC分类号： C07K14/34 ,  C12P13/08 ,  C12N1/21

CPC分类号： C07K14/34 ,  C12P13/08

公开(公告)号：EP2089525B1

公开(公告)日：2016-03-16

申请号：EP07821770.0

申请日：2007-10-24

申请人： Evonik Degussa GmbH

发明人： BATHE, Brigitte ,  CLAES, Wilfried ,  JERRENTRUP, Silke ,  KREUTZER, Caroline ,  THIERBACH, Georg

IPC分类号： C12N15/77 ,  C12P13/08 ,  C12P13/22 ,  A23K10/40 ,  C12R1/15

CPC分类号： C12N15/77 ,  A23K10/12 ,  A23K20/147 ,  C07K14/34 ,  C12P13/04 ,  C12P13/08 ,  C12P13/227

公开(公告)号：EP2041276A2

公开(公告)日：2009-04-01

申请号：EP07786778.6

申请日：2007-06-20

申请人： Evonik Degussa GmbH

发明人： BATHE, Brigitte ,  CLAES, Wilfried

IPC分类号： C12N9/12

CPC分类号： C12N9/88 ,  C12N9/1217 ,  C12P13/06 ,  C12P13/08 ,  C12Y203/03001 ,  C12Y207/02004

摘要： The invention relates to novel polynucleotides, coding for a polypeptide with citrate synthase activity, bacteria comprising the same, preferably of the species Corynebacterium and Escherichia, and methods for producing amino acids using said bacteria. The invention particularly relates to an isolated polynucleotide, coding for a polypeptide with the amino acid sequence SEQ ID NO:2, wherein the L-aspartic acid in position 5 of the amino acid sequence is replaced by another proteinogenic amino acid and the polypeptide has citrate synthase activity.

公开(公告)号：EP2726615B1

公开(公告)日：2017-03-01

申请号：EP12728293.7

申请日：2012-06-22

申请人： Evonik Degussa GmbH

发明人： RETH, Alexander ,  BATHE, Brigitte ,  HANS, Stephan ,  CLAES, Wilfried

IPC分类号： C12N9/02 ,  C12P13/04 ,  C12N15/77 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12R1/15 ,  C12N9/00

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10