公开(公告)号：EP2799872B1

公开(公告)日：2017-10-18

申请号：EP13166110.0

申请日：2013-05-01

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Drechsler, Andreas ,  Sarofim, Emad ,  Kopp, Martin ,  Cherubini, Claudio ,  Brand-Meier, Judith ,  Oosterbroek, Edwin ,  Gwerder, Nicole

IPC分类号： G01N33/543

CPC分类号： G01N1/44 ,  C12M33/00 ,  G01N33/54353

摘要： A method of individually releasing from an entity one or more members of a sub-group of biological units comprised in a heterogeneous group of biological units is provided. The method comprises binding said group of biological units comprising said sub-group of biological units to said entity via a linker. Following binding, the location of said one or more members on said entity is determined. Once the location is determined, a localized physical pulse is applied to said one or more members. The localized physical pulse individually releases the one or more members from the entity by dissociating the linker.

公开(公告)号：EP2263802A1

公开(公告)日：2010-12-22

申请号：EP10160680.4

申请日：2010-04-22

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Sarofim, Emad ,  Kopp, Martin ,  Cherubini, Claudio

IPC分类号： B01L9/00 ,  G01N35/02

CPC分类号： G01N35/1083 ,  B01L9/52 ,  B01L9/523 ,  B01L2300/0829 ,  G01N35/1065

摘要： The present invention pertains to a system for dispensing one or more fluids into wells of a target multi-well plate, comprising a holder for holding said multi-well plate in a predefined holding position, said plate having a well region provided with plural wells for accommodating said fluids and an edge region surrounding said well region, said holder including: a contact area adapted to contact said edge region for forming a sealing zone air-tightly sealing a void formed between said holder and said plate; at least one supporting face adapted to support said well region in a planar condition; a duct for connection to a pump for generating a negative pressure in said void so as to draw said well region onto said at least one supporting face. It further relates to such holder. It yet further relates to a method for dispensing fluids into wells of a target multi-well plate, comprising the following steps of: providing such holder and a multi-well plate in holding position and generating a negative pressure in said void so as to pull said well region onto said at least one supporting face.

摘要翻译： 本发明涉及一种用于将一种或多种流体分配到目标多孔板的井中的系统，包括用于将所述多孔板保持在预定保持位置的保持器，所述板具有设置有多个井的井区， 容纳所述流体和围绕所述井区域的边缘区域，所述保持器包括：适于接触所述边缘区域的接触区域，用于形成气密密封形成在所述保持器和所述板之间的空隙的密封区域; 至少一个支撑面，适于在平坦状态下支撑所述阱区; 用于连接到泵的管道，用于在所述空隙中产生负压，以便将所述井区域吸引到所述至少一个支撑面上。 它还涉及这种保持器。 它还涉及一种用于将流体分配到目标多孔板的孔中的方法，包括以下步骤：将保持器和多孔板提供在保持位置并在所述空隙中产生负压以拉动 所述井区域到所述至少一个支撑面上。

公开(公告)号：EP1712903A1

公开(公告)日：2006-10-18

申请号：EP05007912.8

申请日：2005-04-11

申请人： Roche Diagnostics GmbH ,  F. HOFFMANN-LA ROCHE AG

发明人： Kopp, Martin ,  Curcio, Mario

IPC分类号： G01N27/447

CPC分类号： G01N27/44773 ,  G01N27/44795

摘要： A process and a device for the separation of a sample mixture (1) for analytical reason by two-dimensional gel electrophoresis. The method is involving a first separation in a first gel strip (7) by isoelectric focusing and a second separation in a second gel to determine the molecular size of the compounds. When starting the separation in the second dimension the buffer solution for transferring the compounds separated in the first dimension into the second dimension gel is containing sodium-dodecyl-sulphate (SDS) and by applying an electric field the SDS migrates electrokinetically into the first gel strip (7), and the compounds are being complexed simultaneously with SDS. After forcing the SDS into the first gel strip (7) for complexation of the components at least the same or a higher electric field is applied when starting the migration of the complexed components into the second gel.

摘要翻译： 用于通过二维凝胶电泳的分析原因分离样品混合物（1）的方法和装置。 该方法涉及通过等电聚焦在第一凝胶条（7）中进行第一分离，并且在第二凝胶中进行第二次分离以确定化合物的分子大小。 当在第二维开始分离时，用于将在第一维中分离的化合物转移到第二维凝胶中的缓冲溶液含有十二烷基硫酸钠（SDS），并且通过施加电场，SDS电动迁移到第一凝胶条 （7），并且化合物与SDS同时络合。 在将SDS加入到第一凝胶条（7）中以使组分络合时，当将复合组分迁移到第二凝胶中时，施加至少相同或更高电场的SDS。

公开(公告)号：EP1666150A1

公开(公告)日：2006-06-07

申请号：EP05024993.7

申请日：2005-11-16

申请人： Roche Diagnostics GmbH ,  F. HOFFMANN-LA ROCHE AG

发明人： Kopp, Martin

IPC分类号： B01L7/00 ,  C12Q1/68

CPC分类号： B01L7/52 ,  B01L3/5021 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0688

摘要： The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method allows to speed up overall reaction time without affecting the limit of detection.

摘要翻译： 本发明涉及通过使样品经受热循环从模板核酸制备核酸的方法，装置和计算机程序。 在第一数量的热循环之后，部分量的反应混合物经受第二数量的热循环。 这两步扩增方法可以加快整个反应时间，而不影响检测限。

公开(公告)号：EP2799872A1

公开(公告)日：2014-11-05

申请号：EP13166110.0

申请日：2013-05-01

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Drechsler, Andreas ,  Sarofim, Emad ,  Kopp, Martin ,  Cherubini, Claudio ,  Brand-Meier, Judith ,  Oosterbroek, Edwin ,  Gwerder, Nicole

IPC分类号： G01N33/543

CPC分类号： G01N1/44 ,  C12M33/00 ,  G01N33/54353

摘要： A method of individually releasing from an entity one or more members of a sub-group of biological units comprised in a heterogeneous group of biological units is provided. The method comprises binding said group of biological units comprising said sub-group of biological units to said entity via a linker. Following binding, the location of said one or more members on said entity is determined. Once the location is determined, a localized physical pulse is applied to said one or more members. The localized physical pulse individually releases the one or more members from the entity by dissociating the linker.

摘要翻译： 提供了从实体单独地释放包含在生物单元的异质组中的生物单元子组的一个或多个成员的方法。 该方法包括通过接头将包含所述生物单元亚组的所述生物单元组结合到所述实体。 在绑定之后，确定所述一个或多个成员在所述实体上的位置。 一旦确定了位置，则将局部物理脉冲施加到所述一个或多个成员。 本地化的物理脉冲通过解离接头单独地从实体释放一个或多个成员。

公开(公告)号：EP1637228A1

公开(公告)日：2006-03-22

申请号：EP04021997.4

申请日：2004-09-16

申请人： Roche Diagnostics GmbH ,  F. HOFFMANN-LA ROCHE AG

发明人： Kopp, Martin ,  Sarofim, Emad

IPC分类号： B01L7/00 ,  C12Q1/68

CPC分类号： G05D23/19 ,  B01L7/52 ,  B01L2300/1822 ,  B01L2300/1833

摘要： A method to adjust a temperature of a fluid (8) is disclosed, the method comprising the steps of providing a heat sink (4) taking effect on the fluid (8), heating the fluid (8) by Joule heating, and adjusting the heat provided by Joule heating in order to reach a desired temperature of the fluid (8). Furthermore, an apparatus to carry out the method is also disclosed. Therewith, a very efficient temperature change of the fluid can very fast be obtained since the temperature of the surrounding is not substantially changed by heat diffusion. While a temperature increase is carried out by Joule heating, a temperature decrease is carried out by simply deactivating Joule heating. As soon as Joule heating is deactivated, the heat is extracted from the fluid by the heat sink, thereby initiating a high speed temperature change towards lower temperatures.

摘要翻译： 公开了一种调节流体（8）的温度的方法，所述方法包括以下步骤：提供对流体（8）起作用的散热器（4），通过焦耳加热来加热流体（8），并调节 由焦耳加热提供的热量以达到流体（8）的期望温度。 此外，还公开了一种执行该方法的装置。 因此，由于通过热扩散基本上不改变周围的温度，所以可以非常快速地获得流体的非常有效的温度变化。 当通过焦耳加热进行温度升高时，通过简单地停用焦耳加热来进行温度降低。 焦耳加热失效后，散热器从流体中提取热量，从而开始向较低温度的高速温度变化。

公开(公告)号：EP2436446B1

公开(公告)日：2016-09-21

申请号：EP11183447.9

申请日：2011-09-30

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Oosterbroek, Edwin ,  Kopp, Martin

IPC分类号： B01L3/00

CPC分类号： B01L3/502723 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0409

公开(公告)号：EP2436446A1

公开(公告)日：2012-04-04

申请号：EP11183447.9

申请日：2011-09-30

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Oosterbroek, Edwin ,  Kopp, Martin

IPC分类号： B01L3/00

CPC分类号： B01L3/502723 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0409

摘要： A multi-chamber plate (110) is disclosed: preferably for analytical purposes, the multi-chamber plate (110) has a plurality of chambers (112) and a channel system (114) for filling the chambers (112) with at least one sample fluid (113). The multi-chamber plate (110) has a proximal end (116) and a distal end (118). A radial direction (120) is defined from the proximal end (116) to the distal end (118). A centrifugal force (122) is applicable parallel to the radial direction (120). The channel system (114) comprises at least one application site (124) for applying the sample fluid (113) to the channel system (114). The chambers (112) each have at least one inlet opening (126) and at least one outlet opening (128) being separate from the inlet opening (126). Both the inlet opening (126) and the outlet opening (128) are positioned on a proximal side (130) of the chambers (112). The chambers (112) are fillable through the inlet openings (126) with the sample fluid (113) driven by the centrifugal force (122). The chambers (112) are vented through the outlet openings (128). At least one reagent (200) is located in the chambers (112). The channel system (114) has at least one main feeding line (132) and has at least one main venting line (134). The inlet openings (126) are connected to the main feeding line (132) at inlet channel junctions (136) and the outlet openings (128) are connected to the main venting line (134) at outlet channel junctions (138). The inlet channel junctions (136) are located further towards the distal end (118) than the respective outlet channel junctions (138). The inlet openings (126), the outlet openings (128), the inlet channel junctions (136) and the outlet channel junctions (138) are arranged such that, during the filling with the sample fluid (113), a venting of each chamber (112) is possible until the respective chamber (112) is completely filled with the sample fluid (113). Described layouts provide controlled, complete filling of the chambers (112) while strongly reducing the risk of cross-contamination of reagents (200), contained in the chambers (112), and trapping of gas bubbles in the chambers (112).

摘要翻译： 的多腔室板（110）是圆盘游离缺失：用于与至少一个填充所述室（112），优选用于分析目的，该多室板（110）具有腔（112）多个和一个信道系统（114） 样本流体（113）。 多室板（110）具有近端（116）和远端（118）。 径向方向（120）从近端（116）到远端（118）所定义。 的离心力（122）适用于平行于径向方向（120）。 通道系统（114）包括至少一个应用程序的网站（124），用于将样品流体（113）到信道系统（114）。 所述腔室（112）各具有至少一个入口开口（126）和至少一个出口开口（128）是从所述入口开口（126）是分开的。 两个入口开口（126）和出口开口（128）被定位在所述腔室（112）的基端侧（130）。 所述腔室（112）是通过所述入口开口（126）与由所述离心力（122）驱动的样本流体（113）可填充。 所述腔室（112）通过所述出口开口（128）排出。 至少一种试剂（200）位于所述腔室（112）。 通道系统（114）具有至少一个主馈电线（132）和具有至少一个主排气管线（134）。 所述入口开口（126）被连接到在入口通道结（136），并连接到在出口通道结（138）的主排气管线（134）的出口开口（128）的主馈送线（132）。 入口通道结（136）被进一步定位成朝向所述远端（118）比所述respectivement出口通道结（138）。 所述入口开口（126），出口开口（128），所述入口通道结（136）和出口通道连接（138）被布置寻求的是，与样品流体（113），每个腔室的排气填充期间 （112）是可能的，直到respectivement室（112）被完全填充呼叫与样品流体（113）。 描述的布局提供控制，腔室（112），同时强烈地减少包含在所述腔室（112）的试剂（200）的交叉污染，和气泡捕获在腔室中的风险（112）的完全填充。

公开(公告)号：EP1525916A1

公开(公告)日：2005-04-27

申请号：EP03024419.8

申请日：2003-10-23

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Griss, Patrick ,  Siljegovic, Vuk ,  Kopp, Martin

IPC分类号： B01L3/00 ,  B01J19/00

CPC分类号： F16K99/0017 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0688 ,  F16K99/0001 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0084

摘要： The invention is related to a microfluidic device through which a liquid (19) is conveyed, having at least one non-closing valve (1, 65, 73) and a channel system (10, 12, 14; 43, 44; 63, 64) within which a channel (12) branches-off from a first channel (10) connected to a liquid supply (81, 91, 101). The channel (12) branches-off from the first channel (10) prior to the at least one non-closing valve (1, 65, 73) and is connected to the outlet of the non-closing valve (1, 65, 73) channel (14) downstream of the non-closing valve (1, 65, 73). The microfluidic device (80, 90, 100) may comprise a functional chamber (40).

摘要翻译： 本发明涉及一种微流体装置，液体（19）通过该微流体装置被输送，具有至少一个非关闭阀（1,65,73）和通道系统（10,12,14; 43,44; 其中通道（12）从连接到液体供应源（81,91,101）的第一通道（10）分支出来。 在至少一个非关闭阀（1,67,73）之前，通道（12）从第一通道（10）分支出来并连接到非关闭阀（1,67,73）的出口 ）通道（14）在非关闭阀（1,65,73）的下游。 微流体装置（80,90,100）可以包括功能室（40）。

公开(公告)号：EP1371419A1

公开(公告)日：2003-12-17

申请号：EP02077317.2

申请日：2002-06-12

申请人： F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

发明人： Kopp, Martin

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/68

CPC分类号： B01L3/5027 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/086

摘要： A method and a device for determining the presence or the amount of an analyte in a test sample.
The method comprises:

(a) introducing a test sample in a chamber (12),
(b) performing the following steps in that chamber (12):

(b.1) capturing nucleic acids contained in the test sample, said capturing being obtained by use of a binding surface (15) located within said chamber, said binding surface having a high binding affinity for capturing nucleic acids,
(b.2) amplifying a target nucleic acid sequence which is part of the captured nucleic acids, and
(b.3) detecting the presence of the amplified target nucleic acid sequence.


The device comprises the chamber (12). This chamber has a sealable inlet port (13) for introducing into chamber (12) a liquid containing the test sample and a sealable outlet port (14) allowing the exit of liquid from said chamber (12). Chamber (12) contains a binding surface which is adapted to capture nucleic acids contained in the test sample when said liquid containing said test sample flows through said chamber (12). Chamber (12) has at least one wall (16) which enables heating and cooling of the contents of said chamber (12), the temperature of said chamber (12) being thereby modifiable in order to carry out therein a process for amplifying a target nucleic acid sequence which is part of the captured nucleic acids. A part of chamber (12) has a zone (17) which allows examination of the chamber contents by optical means for detecting the presence of an amplified target nucleic acid sequence contained in said chamber (12).

摘要翻译： 用于确定测试样品中分析物的存在或量的方法和装置。 该方法包括：（a）在室（12）中引入测试样品，（b）在该室（12）中执行以下步骤：（b.1）捕获测试样品中所含的核酸，获得所述捕获 通过使用位于所述室内的结合表面（15），所述结合表面对捕获核酸具有高结合亲和力，（b.2）扩增作为捕获核酸一部分的靶核酸序列，和（b 3）检测扩增的靶核酸序列的存在。 该装置包括腔室（12）。 该室具有可密封的入口（13），用于将容纳测试样品的液体和允许从所述室（12）排出液体的可密封出口（14）引入室（12）。 当所述含有所述测试样品的液体流过所述室（12）时，所述室（12）含有粘合表面，所述结合表面适于捕获包含在所述测试样品中的核酸。 室（12）具有至少一个壁（16），其能够加热和冷却所述室（12）的内容物，因此可以修改所述室（12）的温度，以便在其中执行放大目标的过程 作为捕获的核酸的一部分的核酸序列。 腔室（12）的一部分具有区域（17），其允许通过光学装置检查腔室内容物，以检测包含在所述腔室（12）中的扩增的靶核酸序列的存在。