摘要:
Methods and systems for performing single molecule amplification for detection, quantification and statistical analysis of nucleic acids are provided. Methods and systems are provided for determining and quantifying lengths of nucleic acids of interest.
摘要:
A minute flow path is filled with liquid so that the liquid may be supplied from an accumulating portion. Energy is imparted to the liquid exposed outwardly of an opening in the flow path by a heat generating element or by energy application to thereby heat and gasify the liquid. Thereupon, the liquid is supplied by an amount corresponding to the gasified liquid by capillary phenomenon through the flow path, and gasification is continuously effected, whereby a flow free of pulsating flow can be formed in the flow path.
摘要:
[Problem] To provide a PCR device and a PCR method capable of achieving a reduction in size and volume and even heating of a channel. [Solution] A reaction container 10 of the PCR device 1 has a tubular ring channel 20 formed by a substrate 11, a channel forming plate 12, and a cover plate 13. The ring channel 20 comprises an electrode pair 21 disposed, in a diametrical direction of an inner surface of one side wall of the ring channel 20, to face each other with a horizontal gap interposed therebetween, and is vertically erected. The gap length of the electrode pair 21 is across the entire width within a cross-section of the ring channel 20.
摘要:
Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
摘要:
Provided is a method comprising adding anions having a high charge density to a liquid medium; the liquid medium comprising molecules that hydrogen bond with one another; the anions interacting with the molecules of the liquid medium with a force that is stronger than the forces that produce hydrogen bonding between the molecules of the medium; and heating the liquid medium by irradiating it with microwaves.
摘要:
Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and systems for performing a wide variety of fluid operations are provided. The devices and systems are used in performing fluid operations which require a large number of iterative, successive or parallel fluid manipulations, in a microscale, or sealed and readily automated format.
摘要:
A method to adjust a temperature of a fluid (8) is disclosed, the method comprising the steps of providing a heat sink (4) taking effect on the fluid (8), heating the fluid (8) by Joule heating, and adjusting the heat provided by Joule heating in order to reach a desired temperature of the fluid (8). Furthermore, an apparatus to carry out the method is also disclosed. Therewith, a very efficient temperature change of the fluid can very fast be obtained since the temperature of the surrounding is not substantially changed by heat diffusion. While a temperature increase is carried out by Joule heating, a temperature decrease is carried out by simply deactivating Joule heating. As soon as Joule heating is deactivated, the heat is extracted from the fluid by the heat sink, thereby initiating a high speed temperature change towards lower temperatures.
摘要:
One aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a conductive member for heating the interior volume. Another aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a plurality of conductive members for heating an interior volume. Another aspect of the invention provides a container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a first conductive member located in the interior volume and adapted to contact a first end of the microfluidic array.