摘要:
A method of simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV is disclosed comprising amplifying the genotypes 1, 2, 3 and/or 4 of HEV with one single non-degenerate forward primer partially overlapping the 5'UTR region of HEV and at least one reverse primer.
摘要:
An operation checking device and checking method for a dispenser, capable of having the dispenser perform a high-reliability, high-accuracy processing. A dispenser comprises one or at least two translucent or semi-translucent liquid passages capable of sucking, discharging and storing liquid, a pressure control means for controlling pressures in the liquid passages, a moving means for relatively moving between a container and the liquid passages, and an operation instruction means for instructing operations to the pressure control means and the moving means, wherein a detection means for detecting the optical conditions of the liquid passages, and their movable areas or parts thereof, and a judgment means for judging the liquid passages-related results of instructions by the operation instruction means based on the optical conditions detected by the detection means are provided to thereby check the operation of the dispenser.
摘要:
Method and apparatus, including computer program products, implement techniques for quantifying a target nucleic acid (target) in a test sample. The techniques include providing a target and a defined initial amount of an internal control nucleic acid (control) different from the target; amplifying the target and control in a common amplification process; measuring a quantity indicating the amount of amplification product for the target and for the control in relation to a parameter representing the progress of the amplification; determining a characteristic value of the progress parameter for the target based on measurement results related to the amount of target amplification product; possibly determining a characteristic valuc of the progress parameter for the control based on measurement results related to the amount of control amplification product; and quantifying the original amount of target according to a predefined or selected quantification scheme using at least the characteristic value for the target.
摘要:
An object is to provide a dispenser operation verification apparatus and verification method that enables operation with high reliability and high accuracy. The construction is such that with a dispenser comprising: one or a plurality of transparent or semi-transparent liquid passages capable of liquid suction, discharge and storage; a pressure control device for controlling the pressure in the liquid passage; a transport device for effecting relative movement between a container and the liquid passage; and an operation instruction device for issuing operating instructions to the pressure control device and the transport device, the operation of the dispenser is verified by providing; a detecting device for detecting an optical condition of the liquid passage, a movable region thereof or a part of that region, and a judgement device for judging the result of the instruction related to the liquid passage issued by the operation instruction device, based on the optical conditions detected by the detecting device.
摘要:
The invention relates to a method of detecting telomerase activity involving the following steps: (a) a test specimen is prepared; (b) a first primer suitable as a telomerase substrate and nucleoside triphosphates are added and the reaction mixture is incubated under conditions conducive to primer extension by the telomerase; (c) amplification of the extension product produced by the telomerase; (d) the amplification product obtained in step (c) is immobilised on a solid phase; and (e) the immobilised amplification product is detected or quantitatively measured. The invention also relates to a reagent kit for carrying out the claimed process.