摘要:
Methods and systems for creating TEM lamella using image restoration algorithms for low keV FIB images are disclosed. An example method includes irradiating a sample with an ion beam at low keV settings, generating (308) a low keV ion beam image of the sample based on emissions resultant from irradiation by the ion beam, and then applying (310) an image restoration model to the low keV ion beam image of the sample to generate a restored image. The sample is then localized (312) within the restored image, and a low keV milling of the sample is performed (316) with the ion beam based on the localized sample within the restored image.
摘要:
The invention relates to a method of sampling a sample (202) and displaying obtained information on a display (244), the method comprising: • Scanning a beam (232) of, for example, electrons, over the sample in a series of N overlapping sub-frames, one sub-frame at a time, each sub-frame comprising M n scan positions, the scan positions of each sub-frame not overlapping with the scan positions of other sub-frames, the beam thereby irradiating the sample at N x M n scan positions, the N x M n scan positions forming the field of view; • Using a detector (242) detecting a signal emanating from the sample in response to the irradiation of the sample by said beam, for example secondary or backscattered electrons, said signal sampled for each scan positions; and • Displaying the sub-frames on the display having at least N x M n pixels in such a way, that after the series of N scans each of the pixels displays information derived from the signal from one or more scan positions; Characterized in that • In which after the scan of the first sub-frame each of the pixels displays information derived from the scan positions of the first sub-frame; and • In which after the scan of the second sub-frame each of the pixels displays information derived during the scanning of the first sub-frame, the second sub-frame, or both sub-frames. This results in a scanning method that shows a more even charge distribution over the sample, resulting in e.g. less charging effects, while offering a well interpretable image even after the scanning of the first sub-frame.
摘要:
A method of examining a sample using a charged-particle microscope (400), comprising the following steps: - Mounting the sample (410) on a sample holder (408); - Using a particle-optical column (402) to direct at least one beam (404) of particulate radiation onto a surface S of the sample, thereby producing an interaction that causes emitted radiation to emanate from the sample; - Using a detector arrangement (100, 420) to detect at least a portion of said emitted radiation, which method comprises the following steps: - Embodying the detector arrangement to detect electrons in the emitted radiation; - Recording an output On of said detector arrangement as a function of kinetic energy E n of said electrons, thus compiling a measurement set M = {(O n , E n )} for a plurality of values of E n ; - Using computer processing apparatus (424) to automatically deconvolve the measurement set M and spatially resolve it into a result set R = {(V k , L k )}, in which a spatial variable V demonstrates a value V k at an associated discrete depth level L k referenced to the surface S, whereby n and k are members of an integer sequence, and spatial variable V represents a physical property of the sample as a function of position in its bulk.
摘要:
The present invention relates to a method of training a network for reconstructing and/or segmenting microscopic images comprising the step of training the network in the cloud. Further, for training the network in the cloud training data comprising microscopic images can be uploaded into the cloud and a network is trained by the microscopic images. Moreover, for training the network the network can be benchmarked after the reconstructing and/or segmenting of the microscopic images. Wherein for benchmarking the network the quality of the image(s) having undergone the reconstructing and/or segmenting by the network can be compared with the quality of the image(s) having undergone reconstructing and/or segmenting by already known algorithm and/or a second network.
摘要:
A method of investigating a specimen using: - A charged-particle microscope comprising: ▪ A specimen holder, for holding the specimen; ▪ A source, for producing a beam of charged-particle radiation; ▪ An illuminator, for directing said beam so as to irradiate a surface of the specimen; ▪ An imaging detector, for receiving a flux of radiation emanating from the specimen in response to said irradiation, so as to produce an image of at least part of said surface; - An apparatus that can be invoked to modify said surface by performing thereon a process chosen from the group comprising material removal, material deposition, and combinations hereof, which method comprises the following steps: - Producing and storing a first image, of a first, initial surface of the specimen; - In a primary modification step, invoking said apparatus so as to modify said first surface, thereby yielding a second, modified surface; - Producing and storing a second image, of said second surface; - Using a mathematical Image Similarity Metric to perform pixel-wise comparison of said second and first images, so as to generate a primary figure of merit for said primary modification step.
摘要:
A method of investigating a specimen using: - A charged-particle microscope comprising: ▪ A specimen holder, for holding the specimen; ▪ A source, for producing a beam of charged-particle radiation; ▪ An illuminator, for directing said beam so as to irradiate a surface of the specimen; ▪ An imaging detector, for receiving a flux of radiation emanating from the specimen in response to said irradiation, so as to produce an image of at least part of said surface;
- An apparatus that can be invoked to modify said surface by performing thereon a process chosen from the group comprising material removal, material deposition, and combinations hereof,
which method comprises the following steps: - Producing and storing a first image, of a first, initial surface of the specimen; - In a primary modification step, invoking said apparatus so as to modify said first surface, thereby yielding a second, modified surface; - Producing and storing a second image, of said second surface; - Using a mathematical Image Similarity Metric to compare said second and first images, so as to generate a primary figure of merit for said primary modification step.
摘要:
A method of accumulating an image of a specimen using a scanning-type microscope, comprising the following steps: - Directing a beam of radiation from a source through an illuminator so as to irradiate a surface S of the specimen; - Using a detector to detect a flux of radiation emanating from the specimen in response to said irradiation; - Causing said beam to follow a scan path relative to said surface; - For each of a set of sample points in said scan path, recording an output D n of the detector as a function of a value P n of a selected measurement parameter P, thus compiling a measurement set M = {(D n , P n )}, where n is a member of an integer sequence; - Using computer processing apparatus to automatically deconvolve the measurement set M and spatially resolve it into a result set R representing depth-resolved imagery of the specimen,
whereby, considered at a given point p i within the specimen, the method comprises the following steps: - In a first probing session, employing a first beam configuration B 1 to irradiate the point p i with an associated first Point Spread Function F 1 , whereby said beam configuration is different to said measurement parameter; - In at least a second probing session, employing a second beam configuration B 2 to irradiate the point p i with an associated second Point Spread Function F 2 , whereby F 2 overlaps partially with F 1 in a common overlap zone O i in which point p i is located; - Using an Independent Component Analysis algorithm in said computer processing apparatus to perform spatial resolution in said overlap zone O i .
摘要:
The invention relates to a multi-beam apparatus (210) for inspecting a sample (232) with at least a first focused beam and a second focused beam, the apparatus equipped to scan the first and the second beam (243, 218) over the sample, the apparatus equipped with at least one detector (240) for detecting secondary radiation emitted by the sample when said sample is irradiated by the first and the second beam, the detector capable of outputting a detector signal representing the intensity of the secondary radiation detected by the detector, in working the detector signal comprising information caused by the first and the second beam, characterized in that the apparatus is equipped with a programmable controller (219) programmed for processing the detector signal using a source separation technique, as a result of which the programmable controller is equipped to output a signal representing information caused by a single beam.
摘要:
Methods for drift corrected, fast, low dose, adaptive sample imaging with a charged particle microscopy system include scanning a surface region of a sample with a charged particle beam to obtain a first image of the surface region with a first detector modality, and then determining a scan strategy for the surface region. The scan strategy comprises a charged particle beam path, a first beam dwell time associated with at least one region of interest in the first image, the first beam dwell time being sufficient to obtain statistically significant data from a second detector modality, and at least a second beam dwell time associated with other regions of the first image, wherein the first beam dwell time is different than the second beam dwell time. The surface region of the sample is then scanned with the determined scan strategy to obtain data from the first and second detector.