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公开(公告)号：EP3805378A1
公开(公告)日：2021-04-14
申请号：EP18921821.7
申请日：2018-12-20
发明人： YAO, Bin , LUO, Huiying , TU, Tao , HUANG, Huoqing , SU, Xiaoyun , WANG, Yaru , BAI, Yingguo , WANG, Yuan , MENG, Kun
摘要： The present invention takes the glucose oxidase GOD from Aspergillus niger as the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70°C for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80°C for 2 min.
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2.发明公开
公开(公告)号：EP3831948A1
公开(公告)日：2021-06-09
申请号：EP19841706.5
申请日：2019-07-13
发明人： YAO, Bin , SU, XiaoYun , GAO, Fei , LUO, Huiying , HUANG, Huoqing , BAI, Yingguo , WANG, Yuan , TU, Tao , WANG, Yaru , MENG, Kun
IPC分类号： C12N15/80
摘要： The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.
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3.发明公开
公开(公告)号：EP3578647A1
公开(公告)日：2019-12-11
申请号：EP16919766.2
申请日：2016-12-15
发明人： YAO, Bin , LUO, Huiying , LI, Yeqing , WANG, Yuan , TU, Tao , HUANG, Huoqing , BAI, Yingguo , WANG, Yaru , Su, Xiaoyun
摘要： Provided are acidic thermophilic polygalacturonase TePG28A, encoding gene and application thereof. The amino acid sequence thereof is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The expressed acidic thermophilic polygalacturonase by means of cloning has advantages such as high enzyme activity and high stability; can adapt to the high temperature environment in the industrial production; has better application prospect; and can effectively degrade pectic substances such as polygalacturonic acid and pectin; and can be effectively applied to the industrial field of feed, food, and textile, etc.
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4.发明公开
公开(公告)号：EP4006149A1
公开(公告)日：2022-06-01
申请号：EP19939238.2
申请日：2019-11-13
发明人： YAO, Bin , TU, Tao , JIANG, Xiao , LUO, Huiying , SU, Xiaoyun , HUANG, Huoqing , WANG, Yaru , BAI, Yingguo , WANG, Yuan , SHI, Xia , ZHANG, Jie
摘要： The present invention relates to the field of genetic engineering, particularly to a glucose oxidase mutant having improved thermal stability, gene and application thereof. The present invention provides several glucose oxidase GOD mutants with high catalytic efficiency and improved thermal stability, which breaks the barrier of low enzyme activity and poor stability and is suited well to meet the requirements of application to the fields of food, medicine, feed and textile industry, and has a very broad application prospect.
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5.发明公开
公开(公告)号：EP3792356A1
公开(公告)日：2021-03-17
申请号：EP19799589.7
申请日：2019-05-10
发明人： LUO, Huiying , YAO, Bin , LIU, Weina , GU, Yuan , TU, Tao , WANG, Yuan , WANG, Yaru , HUANG, Huoqing , BAI, Yingguo , SU, Xiaoyun , MENG, Kun
摘要： The present invention relates to mannanase PMan5A mutant having improved heat resistance, gene and application thereof. Said mutant is obtained by substitution the 93 th histidine with tyrosine, the 94 th phenylalanine with tyrosine, the 356 th leucine with histidine, and/or the 389 th alanine with proline. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play the important role for the thermal stability of the mannanase of GH5 family.
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6.发明公开
公开(公告)号：EP3783102A1
公开(公告)日：2021-02-24
申请号：EP19770537.9
申请日：2019-03-21
发明人： YAO, Bin , LUO, Huiying , QIU, Jin , TU, Tao , HUANG, Huoqing , WANG, Yuan , WANG, Yaru , BAI, Yingguo , SU, XiaoYun , MENG, Kun
摘要： The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.
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公开(公告)号：EP3647414A1
公开(公告)日：2020-05-06
申请号：EP18790650.8
申请日：2018-06-07
发明人： YAO, Bin , LUO, Huiying , LIU, Weina , GE, Jianzhong , TU, Tao , HUANG, Huoqing , SU, Xiaoyun , BAI, Yingguo , WANG, Yuan , WANG, Yaru , MENG, Kun
摘要： Provided are a glucose oxidase CnGODA, an encoding gene thereof, a recombinant expression vector comprising the gene, and a recombinant strain; the amino acid sequence of the glucose oxidase CnGODA is as represented in SEQ ID NO.1 or SEQ ID NO.2. Further provided is a method for use in preparing glucose oxidase CnGODA, and application of glucose oxidase CnGODA.
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