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1.发明公开
公开(公告)号：EP3831948A1
公开(公告)日：2021-06-09
申请号：EP19841706.5
申请日：2019-07-13
发明人： YAO, Bin , SU, XiaoYun , GAO, Fei , LUO, Huiying , HUANG, Huoqing , BAI, Yingguo , WANG, Yuan , TU, Tao , WANG, Yaru , MENG, Kun
IPC分类号： C12N15/80
摘要： The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.
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公开(公告)号：EP2021466A1
公开(公告)日：2009-02-11
申请号：EP06741789.9
申请日：2006-04-30
发明人： YAO, Bin , LUO, Huiying , HUANG, Huoqing , WANG, Yaru , YUAN, Tiezheng , SHI, Xiuyun , BAI, Yingguo , MENG, Kun , YANG, Peilong
IPC分类号： C12N9/16
CPC分类号： C07K14/195 , A23K20/189 , A23K50/00 , C12N9/16 , Y10S530/825
摘要： The present invention relates to a novel phytase enzyme, a novel isolated nucleic acid molecule coding the enzyme, and a novel Yersinia intermedia having phytase activity. Particularly, the present invention relates to the phytase having (a) Theoretical molecular weight 45.5 kDa, (b) high specific activity 3960±248 U/mg, (c) high stability at high temperature and wide pH, (d) optimal pH of 4.0-5.0, (e) optimal temperature of 50-60°C, (f) high resistance to pepsin and trypsin. The phytase is very suitable to be used in feed of monogastrics as feed additive. The present invention also relates to a recombinant vector comprising said nucleic acid molecule, a recombinant host cell (e.g., Pichia pastoris ) harboring said recombinant vector, and a method for producing phytase using the recombinant host cell. The present invention further provides a feed additive comprising said phytase and/or host cells expressing a phytase as effective ingredient. In addition, the present invention provides a novel method for isolating phytase from a target organism.
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公开(公告)号：EP3805378A1
公开(公告)日：2021-04-14
申请号：EP18921821.7
申请日：2018-12-20
发明人： YAO, Bin , LUO, Huiying , TU, Tao , HUANG, Huoqing , SU, Xiaoyun , WANG, Yaru , BAI, Yingguo , WANG, Yuan , MENG, Kun
摘要： The present invention takes the glucose oxidase GOD from Aspergillus niger as the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70°C for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80°C for 2 min.
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4.发明公开
公开(公告)号：EP3569704A2
公开(公告)日：2019-11-20
申请号：EP18739340.0
申请日：2018-01-15
发明人： NIU, Canfang , YANG, Peilong , YAO, Bin , LI, Yangyang , YU, Dali , LUO, Huiying , HUANG, Huoqing , WANG, Yaru
摘要： The present invention relates to the field of genetic engineering, particularly to phytase variants YkAPPA having amino acid sequence substituting Leucine at the 162 th site of the sequence set forth in SEQ ID NO.1 with glycine or proline, or having amino acid sequence substituting glutamic acid at the 230 th site of the sequence set forth in SEQ ID NO.1 with glycine, alanine, serine, threonine, aspartic acid, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency of 2.1 times of that of the wild phytase, in the benefit of the development of economical feed enzyme industry.
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公开(公告)号：EP2021466B1
公开(公告)日：2012-08-29
申请号：EP06741789.9
申请日：2006-04-30
发明人： YAO, Bin , LUO, Huiying , HUANG, Huoqing , WANG, Yaru , YUAN, Tiezheng , SHI, Xiuyun , BAI, Yingguo , MENG, Kun , YANG, Peilong
CPC分类号： C07K14/195 , A23K20/189 , A23K50/00 , C12N9/16 , Y10S530/825
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6.发明公开
公开(公告)号：EP3578647A1
公开(公告)日：2019-12-11
申请号：EP16919766.2
申请日：2016-12-15
发明人： YAO, Bin , LUO, Huiying , LI, Yeqing , WANG, Yuan , TU, Tao , HUANG, Huoqing , BAI, Yingguo , WANG, Yaru , Su, Xiaoyun
摘要： Provided are acidic thermophilic polygalacturonase TePG28A, encoding gene and application thereof. The amino acid sequence thereof is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The expressed acidic thermophilic polygalacturonase by means of cloning has advantages such as high enzyme activity and high stability; can adapt to the high temperature environment in the industrial production; has better application prospect; and can effectively degrade pectic substances such as polygalacturonic acid and pectin; and can be effectively applied to the industrial field of feed, food, and textile, etc.
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7.发明公开
公开(公告)号：EP3569703A1
公开(公告)日：2019-11-20
申请号：EP18738844.2
申请日：2018-01-15
发明人： NIU, Canfang , YANG, Peilong , YAO, Bin , LI, Yangyang , DU, Yongkai , LUO, Huiying , HUANG, Huoqing , WANG, Yaru
IPC分类号： C12N9/16 , C12N15/55 , A23K20/189
摘要： The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162 th site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230 th site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.
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8.发明公开A METHOD FOR PRODUCING A PHYTASE VARIANT WITH IMPROVED THERMAL STABILITY, AND A PHYTASE VARIANT AND THE USE THEREOF 有权一种用于生产植酸酶变体热稳定性提高，及其用途植酸酶变体
公开(公告)号：EP3072962A1
公开(公告)日：2016-09-28
申请号：EP13897309.4
申请日：2013-11-12
发明人： YAO, Bin , HUANG, Huoqing , LUO, Huiying , SHAO, Chao , BAI, Yingguo , WANG, Yaru , YANG, Peilong , SHI, Pengjun , MENG, Kun , ZHAO, Heng , MA, Rui
CPC分类号： C12N9/16 , A23K20/189 , C12N1/14 , C12N1/20 , C12N15/52 , C12N15/63 , C12Y301/03
摘要： The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase from Escherichia coli and other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.
摘要翻译： 本发明涉及基因工程领域，具体地讲，本发明涉及一种用于在改进的热稳定性产生具有植酸酶变体的方法，以及一个植酸酶变体及其用途。 植酸酶变体包含至少一个脯氨酸变形例中，相比于来自大肠杆菌和其它突变体肌醇六磷酸酶。 与修饰肌醇六磷酸酶变体具有优选改进的性质，：如热稳定性，最适反应温度，pH值性能，比活性，蛋白酶抗性和性能在动物饲料。
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9.发明公开
公开(公告)号：EP4006149A1
公开(公告)日：2022-06-01
申请号：EP19939238.2
申请日：2019-11-13
发明人： YAO, Bin , TU, Tao , JIANG, Xiao , LUO, Huiying , SU, Xiaoyun , HUANG, Huoqing , WANG, Yaru , BAI, Yingguo , WANG, Yuan , SHI, Xia , ZHANG, Jie
摘要： The present invention relates to the field of genetic engineering, particularly to a glucose oxidase mutant having improved thermal stability, gene and application thereof. The present invention provides several glucose oxidase GOD mutants with high catalytic efficiency and improved thermal stability, which breaks the barrier of low enzyme activity and poor stability and is suited well to meet the requirements of application to the fields of food, medicine, feed and textile industry, and has a very broad application prospect.
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10.发明公开
公开(公告)号：EP3792356A1
公开(公告)日：2021-03-17
申请号：EP19799589.7
申请日：2019-05-10
发明人： LUO, Huiying , YAO, Bin , LIU, Weina , GU, Yuan , TU, Tao , WANG, Yuan , WANG, Yaru , HUANG, Huoqing , BAI, Yingguo , SU, Xiaoyun , MENG, Kun
摘要： The present invention relates to mannanase PMan5A mutant having improved heat resistance, gene and application thereof. Said mutant is obtained by substitution the 93 th histidine with tyrosine, the 94 th phenylalanine with tyrosine, the 356 th leucine with histidine, and/or the 389 th alanine with proline. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play the important role for the thermal stability of the mannanase of GH5 family.
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