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    SEPARATION METHOD USING SINGLE POLYMER PHASE SYSTEMS
    3.
    发明公开
    SEPARATION METHOD USING SINGLE POLYMER PHASE SYSTEMS 有权
    分离使用聚合物相系统

    公开(公告)号：EP2373676A1

    公开(公告)日：2011-10-12

    申请号：EP10729364.9

    申请日：2010-01-07

    申请人： GE Healthcare Bio-Sciences AB

    发明人： VAN ALSTINE, James ,  SHANAGAR, Jamil ,  HJORTH, Rolf ,  HALL, Martin ,  ESTMER NILSSON, Camilla

    IPC分类号： C07K1/14 ,  C07K1/36 ,  C07K16/00 ,  C07K16/04 ,  C07K16/06

    CPC分类号： C07K16/00 ,  C07K1/14 ,  C07K1/36 ,  C07K16/04 ,  C07K16/065 ,  C07K2317/10

    摘要： The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase. With minor or no modification the target containing phase solution can be further processed via standard unit operations such as precipitation, chromatography, and filtration to further purify target and remove any residual polymer.

    PLASMA PROTEIN FRACTIONATION BY SEQUENTIAL POLYACID PRECIPITATION
    7.
    发明公开
    PLASMA PROTEIN FRACTIONATION BY SEQUENTIAL POLYACID PRECIPITATION 审中-公开
    等位多聚肽沉淀分离蛋白质

    公开(公告)号：EP2755992A1

    公开(公告)日：2014-07-23

    申请号：EP12832274.0

    申请日：2012-09-14

    申请人： GE Healthcare Bio-Sciences AB

    发明人： VAN ALSTINE, James ,  BERG, Mikael ,  KJÖRNING, Johanna ,  SHANAGAR, Jamil

    IPC分类号： C07K1/30

    CPC分类号： C07K1/30 ,  A61K35/16 ,  C07K1/303 ,  C07K14/75 ,  C07K14/76 ,  C07K16/18

    摘要： There is a recognized need for novel, more simplified, approaches to isolation of plasma from whole blood, as well as a need to isolate cell-free plasma fractions containing different plasma proteins. Methods are divulged for use of aqueous phase systems, formed in blood or blood containing solutions via addition of a single polymer at relatively low concentration, to effect isolation (clarification) of plasma proteins from blood cells. Methods are also divulged to replace widely used Cohn-type plasma protein fractionation which is based on sequential addition of up to 40% (v/v) ethanol and other precipitants, with simple sequential addition of a polyacid. The latter results in isolation of plasma protein fractions (i.e. fibrinogen, immunoglobulin, albumin) in sequence similar to that obtained with Cohn Fractionation and therefore may be suitable for use to reduce solvent use and solvent-related process complications in existing plasma protein purification processes. It may also support use of polymeric film based containers in novel solvent free plasma fractionation processes. The methods disclosed may also be suitable for use in smaller scale plasma protein isolation, in research and diagnostic applications. The general methodologies are robust and can function over a broad range of process variables such as temperature and pH.

    摘要翻译： 公认需要新的，更简化的从全血中分离血浆的方法，以及需要分离含有不同血浆蛋白的无细胞血浆部分。 通过添加相对较低浓度的单一聚合物在血液或含血液溶液中形成的水​​相体系的使用方法被公开，以从血细胞中分离（澄清）血浆蛋白。 还公开了方法以取代广泛使用的Cohn型血浆蛋白分馏，其基于依次添加高达40％（v / v）乙醇和其他沉淀剂，并且简单地依次添加多元酸。 后者导致以与科恩分级法相似的顺序分离血浆蛋白组分（即纤维蛋白原，免疫球蛋白，白蛋白），因此可适用于减少现有血浆蛋白纯化过程中的溶剂使用和溶剂相关过程并发症。 它也可以支持在新型无溶剂血浆分馏过程中使用基于聚合物膜的容器。 所公开的方法也可适用于研究和诊断应用中的小规模血浆蛋白分离。 一般的方法是稳健的，并且可以在宽范围的过程变量（例如温度和pH）上起作用。