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1.发明公开Nucleic acid sequence amplification methods 失效Verfahren zurVervielfältigungvonNukleinsäuresequenzen。
公开(公告)号：EP0408295A2
公开(公告)日：1991-01-16
申请号：EP90307503.4
申请日：1990-07-10
IPC分类号： C12Q1/68
CPC分类号： C12Q1/706 , C12Q1/6865 , C12Q1/689 , C12Q1/70 , C12Q1/703 , C12Q2600/156 , C12Q2600/158
摘要： Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
摘要翻译： 提供了在基本恒定的温度，离子强度和pH条件下自动催化靶核酸序列的多个拷贝的方法，其中靶序列的多个RNA拷贝自动催化产生另外的拷贝。 这些方法对于产生核酸靶序列的拷贝是有用的，其目的包括定量临床，环境，法医和相似样品中的特异性核酸序列的测定，克隆和产生探针。
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公开(公告)号：EP0408295B1
公开(公告)日：1996-08-28
申请号：EP90307503.4
申请日：1990-07-10
IPC分类号： C12Q1/68
CPC分类号： C12Q1/706 , C12Q1/6865 , C12Q1/689 , C12Q1/70 , C12Q1/703 , C12Q2600/156 , C12Q2600/158
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公开(公告)号：EP0731174A3
公开(公告)日：1998-03-25
申请号：EP96101620.1
申请日：1990-07-10
CPC分类号： C12Q1/706 , C12Q1/6865 , C12Q1/689 , C12Q1/70 , C12Q1/703 , C12Q2600/156 , C12Q2600/158
摘要： Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
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4.发明公开
公开(公告)号：EP0731174A2
公开(公告)日：1996-09-11
申请号：EP96101620.1
申请日：1990-07-10
CPC分类号： C12Q1/706 , C12Q1/6865 , C12Q1/689 , C12Q1/70 , C12Q1/703 , C12Q2600/156 , C12Q2600/158
摘要： Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
摘要翻译： 提供了在基本恒定的温度，离子强度和pH条件下自动催化靶核酸序列的多个拷贝的方法，其中靶序列的多个RNA拷贝自动催化产生另外的拷贝。 这些方法对于产生核酸靶序列的拷贝是有用的，其目的包括定量临床，环境，法医和相似样品中的特异性核酸序列的测定，克隆和产生探针。
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公开(公告)号：EP0731174B1
公开(公告)日：2004-11-17
申请号：EP96101620.1
申请日：1990-07-10
CPC分类号： C12Q1/706 , C12Q1/6865 , C12Q1/689 , C12Q1/70 , C12Q1/703 , C12Q2600/156 , C12Q2600/158
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公开(公告)号：EP0408295A3
公开(公告)日：1991-08-28
申请号：EP90307503.4
申请日：1990-07-10
IPC分类号： C12Q1/68
CPC分类号： C12Q1/706 , C12Q1/6865 , C12Q1/689 , C12Q1/70 , C12Q1/703 , C12Q2600/156 , C12Q2600/158
摘要： Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
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