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    Method for preparation or extracting amino acids from manure
    1.
    发明公开
    Method for preparation or extracting amino acids from manure 失效
    Verfahren zur Herstellung oder Extraktion vonAminosäurenausDünger。

    公开(公告)号：EP0287152A1

    公开(公告)日：1988-10-19

    申请号：EP88200630.7

    申请日：1988-04-05

    申请人： GIST-BROCADES N.V.

    发明人： Slijkhuis, Harmen ,  Sanders, Johan Pieter Marinus

    IPC分类号： C12P13/04 ,  A23K1/16

    CPC分类号： C12P13/12 ,  A23K10/26 ,  A23K20/142 ,  C12P13/04 ,  C12P13/08

    摘要： A method is provided for preparing or extracting amino acids, in particular L-amino acids such as L-lysine and L-methionine from manure. Manure of domestic farm animals is inoculted with bacteria which are capable of producing the amino acids mentioned. The bacteria are allowed to grow in the manure and the reaction mixture is worked up after the fermentation.

    摘要翻译： 提供了一种从粪便中制备或提取氨基酸，特别是L-赖氨酸和L-甲硫氨酸的L-氨基酸的方法。 用能够产生所述氨基酸的细菌接种家养农场动物的粪便。 允许细菌在肥料中生长，并且在发酵后处理反应混合物。

    Molecular cloning and expression in industrial microorganism species
    2.
    发明公开
    Molecular cloning and expression in industrial microorganism species 失效
    Molekulare Klonierung und在工业中的表达Mikroorganismen。

    公开(公告)号：EP0134048A1

    公开(公告)日：1985-03-13

    申请号：EP84200990.4

    申请日：1984-07-06

    申请人： GIST-BROCADES N.V.

    发明人： Sanders, Johan Pieter Marinus ,  van den Berg, Johannes Abel ,  Andreoli, Peter Michael ,  Vos, Yvonne Johanna ,  van Ee, Jan Hendrik ,  Mulleners, Leo J.S.M.

    IPC分类号： C12N15/00 ,  C12N9/28 ,  C12N9/56

    CPC分类号： C12N9/2417 ,  C12N9/54 ,  C12N9/6481 ,  C12N15/63 ,  C12N15/75

    摘要： Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing one or more copies of the gene expressing the polypeptide of interest are introduced into a compatible host, the transformed hosts treated to form protoplasts and then fused with protoplast acceptors prepared from industrial strains. Efficient transfer of the vehicle containing the genes of interest is achieved, with the resulting modified industrial strains being effective producers of the desired polypeptide product.

    摘要翻译： 提供新的方法和新的工业单细胞微生物菌株，特别是工业芽孢杆菌菌株，用于增强内源和外源多肽的产生。 将含有一种或多种表达目的多肽的基因的克隆载体引入相容宿主中，转化的宿主被处理以形成原生质体，然后与由工业菌株制备的原生质体受体融合。 实现含有感兴趣基因的载体的有效转移，所得到的修饰的工业菌株是所需多肽产物的有效生产者。

    Process for the treatment of organic waste
    4.
    发明公开
    Process for the treatment of organic waste 失效
    有机废物处理工艺

    公开(公告)号：EP0565176A3

    公开(公告)日：1993-12-08

    申请号：EP93200931.9

    申请日：1993-03-31

    申请人： GIST-BROCADES N.V.

    发明人： Sanders, Johan Pieter Marinus ,  Van Os, Jan Lambert ,  Jones, Brian Edward

    IPC分类号： C05F3/00 ,  C05F15/00

    CPC分类号： C05F3/00 ,  A23K10/12 ,  Y02A40/205 ,  Y02E50/343 ,  Y02P20/145 ,  Y02P60/877 ,  Y02W30/47 ,  C05F5/00 ,  C05F11/00

    摘要： Organic waste material, which contains at least a substantial part of manure is treated by
    i - separating the organic material in a fraction (A) mainly consisting of the soluble part of said material such as compounds of potassium and nitrogen in solubilized form and a fraction (B) mainly consisting of the (less soluble or) insoluble part of said material such as compounds of P, Ca, and nitrogen in the organic form; ii - sterilizing or pasteurizing fraction (B), followed by an anaerobic fermentation using one or more selected species of microoganisms to, at least partly solubilizing fraction (B) to form a fraction (C) which comprises an increased amount of solubilized nitrogen and a decreased amount of insoluble nitrogen compounds; and iii - wherein fraction (C) is separated into a fraction (D) mainly comprising the soluble compounds such as compounds of nitrogen and into a fraction (E) mainly comprising the insoluble compounds such as compounds of P and Ca.

    Process for the treatment of organic waste
    5.
    发明公开
    Process for the treatment of organic waste 失效
    维尔弗伦

    公开(公告)号：EP0565176A2

    公开(公告)日：1993-10-13

    申请号：EP93200931.9

    申请日：1993-03-31

    申请人： GIST-BROCADES N.V.

    发明人： Sanders, Johan Pieter Marinus ,  Van Os, Jan Lambert ,  Jones, Brian Edward

    IPC分类号： C05F3/00 ,  C05F15/00

    CPC分类号： C05F3/00 ,  A23K10/12 ,  Y02A40/205 ,  Y02E50/343 ,  Y02P20/145 ,  Y02P60/877 ,  Y02W30/47 ,  C05F5/00 ,  C05F11/00

    摘要： Organic waste material, which contains at least a substantial part of manure is treated by

    i - separating the organic material in a fraction (A) mainly consisting of the soluble part of said material such as compounds of potassium and nitrogen in solubilized form and a fraction (B) mainly consisting of the (less soluble or) insoluble part of said material such as compounds of P, Ca, and nitrogen in the organic form;
    ii - sterilizing or pasteurizing fraction (B), followed by an anaerobic fermentation using one or more selected species of microoganisms to, at least partly solubilizing fraction (B) to form a fraction (C) which comprises an increased amount of solubilized nitrogen and a decreased amount of insoluble nitrogen compounds; and
    iii - wherein fraction (C) is separated into a fraction (D) mainly comprising the soluble compounds such as compounds of nitrogen and into a fraction (E) mainly comprising the insoluble compounds such as compounds of P and Ca.

    摘要翻译： 含有至少大部分粪便的有机废物通过以主要由所述材料的可溶部分组成的级分（A）中分离有机材料进行处理，所述部分（A）主要由可溶性形式的钾和氮化合物组成， （B），主要由所述材料（较不溶或不溶）部分组成，例如有机形式的P，Ca和氮的化合物; ii-消毒或巴氏杀菌级分（B），然后使用一种或多种选择的微生物种类进行厌氧发酵，至少部分溶解级分（B）以形成级分（C），其含有增加量的溶解氮和 减少不溶性氮化合物的量; 和iii-其中级分（C）分离成主要包含可溶性化合物如氮化合物的级分（D），并且分离成主要包含不溶性化合物如P和Ca.的化合物的级分（E）。 一个