公开(公告)号：EP2818542A4

公开(公告)日：2015-10-14

申请号：EP12864324

申请日：2012-12-28

申请人： HITACHI CHEMICAL CO LTD ,  UNIV TOKYO NAT UNIV CORP

发明人： KIKUHARA YOSHIHITO ,  KANBARA HISASHIGE ,  SUZUKI TAKAHIRO ,  MATSUNAGA TADASHI ,  YOSHINO TOMOKO ,  HOSOKAWA MASAHITO

IPC分类号： C12M1/28

CPC分类号： G01N1/4005 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/0874 ,  C12M47/02 ,  G01N15/0272 ,  G01N15/06 ,  G01N2001/4088 ,  G01N2015/0065

公开(公告)号：EP2175014A4

公开(公告)日：2015-04-08

申请号：EP08790322

申请日：2008-07-31

申请人： UNIV TOKYO NAT UNIV CORP

发明人： MATSUNAGA TADASHI ,  ARAKAKI ATSUSHI ,  TAGUCHI TOMOYUKI ,  HOSOKAWA MASAHITO

IPC分类号： C12M1/00 ,  C12M1/26 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/53 ,  G01N37/00 ,  C12N5/06

CPC分类号： G01N15/1484 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/049 ,  C12Q1/6841 ,  G01N15/1475 ,  C12Q2565/629

公开(公告)号：EP3214166A4

公开(公告)日：2018-07-04

申请号：EP15853920

申请日：2015-10-29

申请人： NATIONAL UNIV CORPORATION TOKYO UNIV OF AGRICULTURE AND TECHNOLOGY ,  HITACHI CHEMICAL CO LTD

发明人： YOSHINO TOMOKO ,  TANAKA TSUYOSHI ,  MATSUNAGA TADASHI ,  NEGISHI RYO ,  KANBARA HISASHIGE ,  NAKAMURA SEITA

IPC分类号： C12M3/06 ,  C12M1/12 ,  G01N1/36 ,  G01N1/40 ,  G01N33/49 ,  G01N33/50 ,  G01N33/574

CPC分类号： C12M47/04 ,  C12M1/12 ,  C12M1/28 ,  C12M3/06 ,  C12M33/00 ,  C12M33/14 ,  C12M47/02 ,  C12N1/02 ,  G01N1/36 ,  G01N1/4077 ,  G01N33/5091 ,  G01N33/574 ,  G01N2001/4088

摘要： A cell isolation method includes: a cell trapping step of allowing a test liquid to pass through a cell trapping filter which has a plurality of through-holes in the thickness direction, thereby trapping isolation target cells contained in the test liquid on one surface of the cell trapping filter; a gel embedding step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel in which the cells are embedded, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that was hardened in the gel hardening step from the cell trapping filter.