公开(公告)号：EP1674578A4

公开(公告)日：2007-03-14

申请号：EP04817164

申请日：2004-10-14

申请人： HOKKO CHEM IND CO

发明人： YAMAGUCHI MASANORI ,  KITA YUICHI ,  MORI TETSUYA ,  KANBE KENJI ,  TOMODA AKIHIRO ,  TAKAHASHI ATSUSHI ,  ICHIKAWA WAKAKO

IPC分类号： C12N9/04 ,  C07C35/16 ,  C12N1/21 ,  C12N15/53 ,  C12P7/18 ,  C12P7/26

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Further, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

公开(公告)号：EP1072685A4

公开(公告)日：2002-11-27

申请号：EP98914084

申请日：1998-04-17

申请人： HOKKO CHEM IND CO

发明人： TERAKAWA TERUHIKO ,  HASEGAWA HISAKAZU ,  YAMAGUCHI MASANORI

IPC分类号： C12N1/21 ,  C12N9/88 ,  C12N15/60 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/63

CPC分类号： C12N9/88 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8254

摘要： DNA sequences capable of encoding an enzyme protein functioning as a rice dihydrodipicolinate synthase (DHDPS). An example of the above DNA sequences is one having the base sequence represented by SEQ ID NO:1 in the Sequence Listing. Moreover, a DNA having the base sequence represented by SEQ ID NO:5 in the Sequence Listing and one having the base sequence represented by SEQ ID NO:7 each capable of encoding the protein with a DHDPS activity are obtained. By culturing vegetable cells having, transferred thereinto, a vector which carries a DNA fragment having such a DNA integrated into the downstream of a promoter, a transformed plant with a high lysine content can be regenerated.