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    SUBSTRATE FOR BIOCHIP AND BIOCHIP
    1.
    发明公开
    SUBSTRATE FOR BIOCHIP AND BIOCHIP 有权
    BIOCHIPSUBSTRAT UND BIOCHIP

    公开(公告)号：EP1887364A1

    公开(公告)日：2008-02-13

    申请号：EP06756519.2

    申请日：2006-05-24

    申请人： HiPep Laboratories ,  Nippon Light Metal, Co., Ltd.

    发明人： NOKIHARA, Kiyoshi 7-115, Fuujiyama-cho ,  OKA, Yasuo

    IPC分类号： G01N37/00 ,  C12M1/00 ,  C12N15/09 ,  G01N33/53 ,  G01N33/547 ,  G01N33/553

    CPC分类号： G01N33/553 ,  B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  G01N33/54353 ,  G01N33/551 ,  Y10T428/31678

    摘要： A substrate for biochips with which immobilization is easy, which does not exhibit self-fluorescence, which is easy to manufacture, and which is excellent in flatness and surface precision, is disclosed. A substrate having a substrate body of the biochip, which is made of a metal, and a carbon layer having functional groups formed on the metal substrate body is used as a substrate for biochips. Since the substrate body of the substrate for biochips is made of a metal, the substrate is not only easy to manufacture, but also free from cracking and chipping, so that it allows easy handling, and high flatness and surface precision can be attained. Therefore, the problem that the optical system is hard to focus when detecting fluorescence does not occur. Moreover, since the substrate body is made of a metal, it does not emit fluorescence by itself. In addition, since the carbon layer has functional groups such as amino groups, biologically relevant substances can be easily immobilized.

    摘要翻译： 公开了一种容易制造的生物芯片的基板，其固定化容易，不具有自发荧光，易于制造，平坦度和表面精度优异。 作为生物芯片的基板，使用具有由金属制成的生物芯片的基板主体和具有形成在金属基板主体上的官能团的碳层的基板。 由于用于生物芯片的基板的基板由金属制成，所以基板不仅容易制造，而且没有破裂和碎裂，从而易于处理，并且可以获得高平坦度和表面精度。 因此，不发生检测荧光时光学系统难以聚焦的问题。 此外，由于基板主体由金属制成，它本身不会发出荧光。 此外，由于碳层具有诸如氨基的官能团，所以可以容易地固定生物相关的物质。

    BIOCHIP SUBSTRATUM AND METHOD FOR PRODUCTION THEREOF
    3.
    发明公开
    BIOCHIP SUBSTRATUM AND METHOD FOR PRODUCTION THEREOF 有权
    BIOCHIP-SUBSTRAT UND VERFAHREN ZUR HERSTELLUNG DAVON

    公开(公告)号：EP2306197A1

    公开(公告)日：2011-04-06

    申请号：EP09773454.5

    申请日：2009-06-30

    申请人： HiPep Laboratories ,  Nippon Light Metal, Co., Ltd.

    发明人： NOKIHARA, Kiyoshi ,  HIRATA, Akiyoshi ,  OKA, Yasuo ,  TAKEBAYASHI, Yasushi

    IPC分类号： G01N33/543 ,  C12N15/09 ,  G01N33/545 ,  G01N33/551 ,  G01N37/00

    CPC分类号： G01N33/545 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01L3/50 ,  C08J3/28 ,  C12Q1/6837 ,  G01N33/54353 ,  G01N33/54393 ,  Y10T428/273

    摘要： A substrate for biochips which has a high probe loading amounts and a uniform immobilization density, and which further has a high detection sensitivity and a high reproducibility by preventing a non-specific adsorption of proteins, when used as a substrate for biochips for immobilizing probes composed of biologically relevant substances such as proteins and nucleic acids, is disclosed. Amino groups can be bound to the surface of the substrate uniformly, at a high density and stably by covalently immobilizing an amino group-containing polymer on the surface of the substrate. The probe immobilization rate is high and immobilizing density was uniform by immobilizing a probe composed of a biologically relevant substance such as a protein or nucleic acid by utilizing the amino groups. Further, detection sensitivity and reproducibility are high by inhibiting non-specific adsorption of proteins.

    摘要翻译： 用于生物芯片的基底，其具有高的探针加载量和均匀的固定密度，并且当用作用于固定探针的生物芯片的基底时，其进一步具有高的检测灵敏度和通过防止蛋白质的非特异性吸附而具有高的再现性 的生物相关物质如蛋白质和核酸。 氨基可以通过将含氨基的聚合物共价固定在基材的表面上，以高密度和稳定地均匀地结合到基材的表面。 探针固定化率高，固定化密度均匀，通过利用氨基固定由生物相关物质如蛋白质或核酸组成的探针。 此外，通过抑制蛋白质的非特异性吸附，检测灵敏度和再现性高。

    BIOCHIP SUBSTRATE AND BIOCHIP
    6.
    发明公开
    BIOCHIP SUBSTRATE AND BIOCHIP 有权
    生物芯片衬底和生物芯片

    公开(公告)号：EP2006679A2

    公开(公告)日：2008-12-24

    申请号：EP06766807.9

    申请日：2006-06-16

    申请人： HiPep Laboratories ,  Nippon Light Metal Company Ltd.

    发明人： NOKIHARA, Kiyoshi ,  OKA, Yasuo

    IPC分类号： G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  G01N33/543 ,  G01N33/547 ,  G01N37/00

    CPC分类号： G01N33/54393 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/12 ,  B82Y30/00 ,  C40B60/14

    摘要： A substrate for biochips with which spots are not diffused and cross-contamination due to contacts between adjacent spots does not occur, as well as a biochip using the substrate, is disclosed. A substrate for biochips with which spots are not diffused and cross-contamination due to contacts between adjacent spots does not occur, by forming a plurality of recesses for immobilizing biologically relevant substances, as well as a biochip using the substrate, was provided. It was discovered also that the desired binding of target molecules in a test sample occurs at a detectable level in solution systems even if the volume of each recess is extremely small as small as 1 nL to 10 nL.

    摘要翻译： 公开了一种用于生物芯片的基板，该基板不会发生点扩散，并且由于相邻点之间的接触而不会发生交叉污染，以及使用该基板的生物芯片。 通过形成多个用于固定生物相关物质的凹槽以及使用该基板的生物芯片，提供了一种用于生物芯片的基板，该基板不会发生斑点扩散并且由于相邻斑点之间的接触而不会发生交叉污染。 还发现，即使每个凹槽的体积非常小（小至1nL至10nL），测试样品中目标分子的期望结合在溶液系统中以可检测的水平发生。