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    DNA SEQUENCING
    3.
    发明公开
    DNA SEQUENCING 有权
    DNA-SEQUENZIERUNG

    公开(公告)号：EP2844772A4

    公开(公告)日：2016-01-20

    申请号：EP13784766

    申请日：2013-05-02

    申请人： IBIS BIOSCIENCES INC

    发明人： ESHOO MARK W

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2525/101 ,  C12Q2525/185 ,  C12Q2563/107

    摘要： Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to methods, compositions, systems, and kits for sequencing a nucleic acid using a degenerate two-base code. Particular embodiments provide: 1) that the two-base degenerate code relates a first element to a base comprising adenine (A) or guanine (G) and a second element to a base comprising cytosine (C) or thymine (T); 2) that the two-base degenerate code relates a first element to a base comprising A or C and a second element to a base comprising G or T; and 3) that the two-base degenerate code relates a first element to a base comprising G or C and a second element to a base comprising A or T.

    摘要翻译： 本文提供了与核酸测序相关的技术，特别但并非排他地涉及使用简并二碱基编码对核酸进行测序的方法，组合物，系统和试剂盒。 具体实施方案提供：1）所述双碱基简并代码将第一元件与包含腺嘌呤（A）或鸟嘌呤（G）的碱和第二元素连接至包含胞嘧啶（C）或胸腺嘧啶（T）的碱; 2）两碱基简并代码将第一元素与包含A或C的碱和第二元素相结合，包括G或T; 和3）两碱性简并代码将第一元素与包含G或C的碱和第二元素相关联到包含A或T的碱。

    NUCLEIC ACID SAMPLE PREPARATION METHODS AND COMPOSITIONS
    8.
    发明公开
    NUCLEIC ACID SAMPLE PREPARATION METHODS AND COMPOSITIONS 有权
    VERFAHREN UND ZUSAMMENSETZUNGENFÜRDIE HERSTELLUNG VONNUKLEINSÄUREPROBEN

    公开(公告)号：EP2658988A4

    公开(公告)日：2014-10-22

    申请号：EP11853018

    申请日：2011-12-27

    申请人： IBIS BIOSCIENCES INC

    发明人： ESHOO MARK W ,  PICURI JOHN ,  MOTLEY STANLEY

    IPC分类号： C12P19/34

    CPC分类号： B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/686 ,  C12Q2521/501 ,  C12Q2525/191

    摘要： The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification.

    摘要翻译： 本发明提供了用于在多用途缓冲液（例如，使用全基因组扩增）中制备核酸文库的组合物和方法，其中在步骤之间或期间不需要核酸纯化。 在某些实施方案中，使用少量的起始核酸（例如，基因组DNA），并且这些步骤在单个容器中完成。 在一些实施方案中，对核酸文库进行测序方法学或滚环扩增。

    PRODUCTION OF SINGLE-STRANDED CIRCULAR NUCLEIC ACID
    9.
    发明公开
    PRODUCTION OF SINGLE-STRANDED CIRCULAR NUCLEIC ACID 有权
    制备单链环状核酸

    公开(公告)号：EP2545183A4

    公开(公告)日：2014-08-13

    申请号：EP11754012

    申请日：2011-03-09

    申请人： IBIS BIOSCIENCES INC

    发明人： ESHOO MARK W ,  PICURI JOHN

    IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

    CPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/66 ,  C12P19/34 ,  C12Q2521/501 ,  C12Q2521/519 ,  C12Q2525/301 ,  C12Q2525/307 ,  C40B40/08 ,  C40B50/06

    摘要： A method is provided for generating single stranded circular nucleic acid from a sample of target nucleic acid. A complex comprising a transposase and a plurality of hairpin polynucleotides is formed with each of the hairpin polynucleotides having a duplex region comprising a transposase recognition sequence. The complex is mixed with the target nucleic acid, thereby fragmenting the target nucleic acid and ligating the hairpin polynucleotides to the target nucleic acid to form hairpin-linked nucleic acid fragments, each having a nucleobase segment gap between each fragment and its corresponding hairpin polynucleotide. The hairpin-linked fragments are contacted with a ligase, thereby ligating the hairpin-linked fragments together to form single-stranded circular nucleic acid comprising a pair of opposing loops and an intervening duplex region comprising a pair of nucleobase segment gaps. The single-single stranded circular nucleic acid is then contacted with a polymerase and nucleotide triphosphates, thereby filling the nucleobase segment gaps.