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    PROCEDE DE PRODUCTION DE SUBSTRAT LIGNOCELLULOSIQUE LIQUEFIE OPTIMISE
    4.
    发明公开
    PROCEDE DE PRODUCTION DE SUBSTRAT LIGNOCELLULOSIQUE LIQUEFIE OPTIMISE 审中-公开
    VERFAHREN ZUR HERSTELLUNG EINES OPTIMIERTENVERFLÜSSIGTENLIGNOCELLULOSE-SUBSTRATES

    公开(公告)号：EP2791346A2

    公开(公告)日：2014-10-22

    申请号：EP12813406.1

    申请日：2012-11-29

    申请人： IFP Energies nouvelles

    发明人： LOURET, Sylvain ,  ROUSSET, Romain

    IPC分类号： C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C13K1/02 ,  G01N11/00 ,  G01N27/02 ,  G01N3/26

    摘要： The present invention relates to a production of liquefied lignocellulosic substrate by enzymatic reaction in which 10% to 40% by weight of solids of lignocellulosic substrate pretreated with water and with enzymes at a concentration between 0.1 and 60 mg of enzymes per gram of cellulose are brought into contact, with stirring, for a duration of between 1 and 24 hours, said process being characterized in that over time at least the value of one of the rheological characteristics of the reaction medium is measured and in that if a reduction of said value over time is detected, a step a) below is carried out: a) the flow rate for the supply of pretreated lignocellulosic substrate is increased, with or without modification of the flow rate of enzymes and/or of water, and in that if an increase of said value over time is detected, a step b) below is carried out: b) the flow rate for the supply of water and/or of enzymes is increased, with or without modification of the flow rate of pretreated lignocellulosic substrate.

    摘要翻译： 本发明涉及通过酶反应生产液化的木质纤维素底物。 将预处理的木质纤维素底物的10重量％至40重量％的干物质与水和酶在0.1至60毫克酶/克纤维素中接触1至24小时。 随着时间的推移，至少测量反应介质的一个流变特性的值。 如果随着时间的推移检测到值的降低，则执行以下步骤a）：a）预处理的木质纤维素底物的进料流速增加，在或不改变酶和/或水的流速的情况下; 如果随时间检测到值的增加，则执行步骤b）：b）增加水和/或酶的进料流速，同时或不改变预处理的木质纤维素底物的流速。

    PROCÉDÉ DE PRODUCTION D'UN COCKTAIL ENZYMATIQUE UTILISANT LES RÉSIDUS SOLIDES D'UN PROCÉDÉ DE CONVERSION BIOCHIMIQUE DE MATÉRIAUX LIGNO-CELLULOSIQUES
    9.
    发明公开
    PROCÉDÉ DE PRODUCTION D'UN COCKTAIL ENZYMATIQUE UTILISANT LES RÉSIDUS SOLIDES D'UN PROCÉDÉ DE CONVERSION BIOCHIMIQUE DE MATÉRIAUX LIGNO-CELLULOSIQUES 有权
    用于生产酶混合物与固体废料PROCESS FOR木质纤维素材料的生物化学转化

    公开(公告)号：EP2791328A1

    公开(公告)日：2014-10-22

    申请号：EP12813901.1

    申请日：2012-11-27

    申请人： IFP Energies nouvelles

    发明人： BEN CHAABANE, Fadhel ,  LOURET, Sylvain

    IPC分类号： C12N9/42 ,  C12N9/24 ,  C12N1/16 ,  C12P7/10

    CPC分类号： C12N9/2437 ,  C12N1/16 ,  C12N9/2477 ,  C12P7/10 ,  C12P21/00 ,  C12P2203/00 ,  C12Y302/01004 ,  C12Y302/01074 ,  Y02E50/16

    摘要： The present invention relates to a method for producing an enzyme cocktail in a submerged culture with a cellulolytic microorganism, including two phases: a phase a) of growing said microorganism in the presence of at least one carbon growth substrate in a closed reactor, the concentration of said carbon growth substrate in said growing phase being between 10 and 90 g/L; and a phase b) for producing the enzyme cocktail in which at least one inducing carbon substrate is fed, said inducing carbon substrate being at least one solid residue from the enzymatic hydrolysis of lignocellulosic materials having undergone a pretreatment step, the concentration of the carbon production substrate in said production phase being between 150 and 400 g/L.

    摘要翻译： 本发明涉及一种用于生产酶鸡尾酒由浸没培养用纤维素分解微生物的方法，包括两个阶段：阶段a）用于在封闭的反应器中，所述至少一种碳质生长基质中存在所述微生物生长的 生长阶段正在开展在10至90g / L的范围内的碳质生长基质的浓度; 一阶段b）用于生产酶促鸡尾酒，其中至少一个碳质诱导底物被提供时，所述碳质诱导物底物是从已经过预处理的木质纤维素材料的酶水解步骤中获得的至少一种固体残余物 步骤，所述生产阶段被在范围内进行与碳质产品衬底的浓度150至400g / L。