公开(公告)号：EP0759976A4

公开(公告)日：2001-02-07

申请号：EP94919078

申请日：1994-03-30

申请人： IGEN INT INC

发明人： SMITH RODGER G ,  MCCAFFERTY JOHN ,  CHISWELL DAVID ,  DARSLEY MICHAEL J ,  FITZGERALD KEVIN ,  KENTEN JOHN H ,  MARTIN MARK T ,  TITMAS RICHARD C ,  WILLIAMS RICHARD O

IPC分类号： G01N33/531 ,  A61K38/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P43/00 ,  C07K16/00 ,  C07K16/44 ,  C12N9/00 ,  C12N15/09 ,  C12N15/10 ,  C12R1/19 ,  C40B40/02 ,  A61K38/43

CPC分类号： C40B40/02 ,  A61K38/00 ,  C07K16/00 ,  C07K16/44 ,  C07K2319/00 ,  C12N9/0002 ,  C12N15/1037 ,  C12N15/1093

公开(公告)号：EP1409459A4

公开(公告)日：2005-08-24

申请号：EP02749629

申请日：2002-06-21

申请人： IGEN INT INC

发明人： SIGAL GEORGE B ,  TJIONG HOWIE ,  DONG LIWEN ,  MASOOD ATHAR ,  TITMAS RICHARD C

IPC分类号： G01N33/532 ,  C07D213/30 ,  C07D213/34 ,  C07D213/40 ,  C07D213/53 ,  C07D213/55 ,  C07D213/81 ,  C07D401/14 ,  C07D471/04 ,  C07F9/58 ,  C07F9/6561 ,  C07F15/00 ,  G01N27/416 ,  G01N33/58 ,  C07D213/22 ,  C07K1/13 ,  C12Q1/68 ,  G01N33/533

CPC分类号： C07D213/30 ,  C07D213/34 ,  C07D213/40 ,  C07D213/81 ,  C07D471/04 ,  C07F9/582 ,  C07F9/6561 ,  C07F15/0053 ,  C09B57/00 ,  C09B57/10 ,  G01N33/582 ,  G01N2458/40

摘要： Bipyridine or phenanthroline ligands presenting functional groups that prevent non-specific binding (in particular, negatively charged functional groups that are unaffected by standard conditions for conjugating biological reagents through amide bonds) are described as are luminescent metal complexes comprising these ligands. The use of luminescent ruthenium and osmium complexes comprising these ligands in electrochemiluminescence assays shows that the use of these labels can significantly reduce the amount of non-specific binding observed relative to assays carried out using reagents labeled with analogous labels that don't present functional groups that decrease non-specific binding.