公开(公告)号：EP0885393A4

公开(公告)日：2000-10-18

申请号：EP97908967

申请日：1997-03-05

申请人： IGEN INT INC

发明人： MASSEY RICHARD J ,  MARTIN MARK T ,  DONG LIWEN ,  LU MING ,  FISCHER ALAN ,  JAMEISON FABIAN ,  LIANG PAM ,  HOCH ROBERT ,  LELAND JONATHON K

IPC分类号： B01J20/281 ,  A61K47/04 ,  B01J20/20 ,  B01J21/18 ,  B01J32/00 ,  C01B31/02 ,  C07K16/44 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N21/78 ,  G01N30/88 ,  G01N33/543 ,  G01N33/553 ,  G01N33/573 ,  G01N33/58 ,  H01M4/60

CPC分类号： C01B31/0213 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/152 ,  C01B32/174 ,  C01B2202/34 ,  C01B2202/36 ,  C07K17/06 ,  D01F9/12 ,  D01F11/14 ,  G01N21/69 ,  G01N21/76 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/582 ,  G01N2030/8863 ,  G11C13/025 ,  H01M4/60 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S977/737 ,  Y10S977/742

摘要： Graphitic nanotubes, which include tubular fullerenes (commonly called 'buckytubes') and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

公开(公告)号：EP0836651A4

公开(公告)日：2004-07-14

申请号：EP96923255

申请日：1996-06-07

申请人： IGEN INT INC

发明人： MARTIN MARK T ,  LIANG PAM ,  DONG LIWEN

IPC分类号： C07D499/26 ,  C12Q1/34 ,  G01N21/78 ,  G01N27/327 ,  G01N27/42 ,  C12Q1/00 ,  A61K51/00 ,  C07D327/10 ,  C12Q1/04 ,  C12Q1/06 ,  G01N31/00 ,  G01N33/53

CPC分类号： C12Q1/34 ,  G01N2333/986 ,  G01N2415/00 ,  G01N2458/30 ,  G01N2458/40

摘要： The present invention provides detectable compounds comprising a chemically-transformable first compound covalently linked to an electrochemiluminescent compound. These compounds are useful in processes and kits that monitor the status of the first compound and derive information from the monitoring. The figure shows a proposed ECL mechanism depicting reaction steps associated with the use of TPA as a nonconjugated reductant.

公开(公告)号：EP0759976A4

公开(公告)日：2001-02-07

申请号：EP94919078

申请日：1994-03-30

申请人： IGEN INT INC

发明人： SMITH RODGER G ,  MCCAFFERTY JOHN ,  CHISWELL DAVID ,  DARSLEY MICHAEL J ,  FITZGERALD KEVIN ,  KENTEN JOHN H ,  MARTIN MARK T ,  TITMAS RICHARD C ,  WILLIAMS RICHARD O

IPC分类号： G01N33/531 ,  A61K38/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P43/00 ,  C07K16/00 ,  C07K16/44 ,  C12N9/00 ,  C12N15/09 ,  C12N15/10 ,  C12R1/19 ,  C40B40/02 ,  A61K38/43

CPC分类号： C40B40/02 ,  A61K38/00 ,  C07K16/00 ,  C07K16/44 ,  C07K2319/00 ,  C12N9/0002 ,  C12N15/1037 ,  C12N15/1093

公开(公告)号：EP0871887A4

公开(公告)日：2000-04-26

申请号：EP96923291

申请日：1996-06-06

申请人： IGEN INT INC

发明人： MARTIN MARK T ,  SAUL RICHARD ,  LIANG PAM

IPC分类号： G01N33/543 ,  C07F15/00 ,  C07K16/44 ,  G01N33/533 ,  G01N33/535 ,  C07K16/00

CPC分类号： G01N33/535 ,  C07F15/0053 ,  C07K16/44 ,  G01N33/533

摘要： Electrochemiluminescent-labels and enzyme substrates, which preferably are conjugated, are used in immunoassays and electrochemiluminescence is generated catalytically. In conventional electrochemiluminescence immunoassays, an anti-analyte antibody molecule can give rise to typically 6-8 electrochemiluminescence-active ruthenium atoms, while in the present invention, each enzyme-labeled anti-analyte molecule can give rise to thousands of electrochemiluminescence-active ruthenium atoms per second. An exemplary immunoassay is based on a catalytic process employing lactamase-conjugated anti-analytes which enzymatically hydrolyze electrochemiluminescent-labeled substrates, making them strongly electrochemiluminescent. The electrochemiluminescence signal generated by each anti-analyte molecule (i.e., each analyte molecule) is much greater than with the conventional method. Accordingly, greater sensitivity can be gained in the measurement of low concentrations of a given immunoassay analyte.

公开(公告)号：EP0837949A4

公开(公告)日：2000-05-17

申请号：EP96923261

申请日：1996-06-06

申请人： IGEN INT INC

发明人： MARTIN MARK T

IPC分类号： C12Q1/32 ,  C12Q1/00 ,  G01N21/66 ,  G01N21/76 ,  G01N21/78 ,  G01N27/327 ,  G01N27/42 ,  G01N33/535 ,  C12N9/04 ,  G01N21/64

CPC分类号： G01N33/535 ,  C12Q1/005 ,  G01N21/66 ,  G01N21/76

摘要： Electrochemiluminescent enzymes, their preparation and use as biosensors are disclosed. Specifically, two appendages are covalently attached to a desired dehydrogenase enzyme; (1) a nicotinamide adenine cofactor or analog thereof, and (2) a luminescent ruthenium complex. For example, glucose concentration is the following way. A doubly-modified glucose dehydrogenase could oxidize glucose with concomitant reduction of the attached NAD to NADH. Because NADH, but not NAD , is able to interact with surface ruthenium to promote ECL, only enzyme molecules that have reacted with glucose will emit light from their ruthenium label in an ECL instrument. The relative close proximity of NADH and ruthenium on the enzyme surface enhances light emission as compared to the same concentrations in free solution. When NADH reduces ruthenium, it returns to become NAD , permitting multiple cycles of ECL light emission from a single enzyme molecule. Such biosensors can be used in solution or bound to a solid surface. Assays employing the biosensor molecules can be performed on an IGEN Origen3 Analyzer.