公开(公告)号：EP2878374A1

公开(公告)日：2015-06-03

申请号：EP13195075.0

申请日：2013-11-29

申请人： IMEC VZW

发明人： Fiorini, Paolo ,  Stakenborg, Tim ,  Colle, Frederik

IPC分类号： B01L3/00 ,  B01L7/00

CPC分类号： B01L3/502784 ,  B01L7/52 ,  B01L2200/061 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/1827 ,  C12Q1/686

摘要： A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.

摘要翻译： 提出了一种用于执行数字PCR的微流体装置100。 该装置包括：半导体衬底; 第一微流体通道104，包括嵌入在半导体衬底中的入口102和出口103; 热耦合到第一微流体通道104的加热元件101; 连接到第一微流体通道104的入口102的液滴发生器107，用于产生液滴并以一定流量将产生的液滴泵入第一微流体通道104; 其特征在于：加热元件101是连接到温度控制单元111的单个加热元件，温度控制单元111被配置为使完整的第一微流体通道104的温度循环至少两个温度值; 并且其中液滴发生器107的流量是适应性的。 此外，使用微流体装置100呈现执行数字PCR的方法。

公开(公告)号：EP2902109B1

公开(公告)日：2018-10-31

申请号：EP15154853.4

申请日：2011-09-23

申请人： IMEC VZW ,  Katholieke Universiteit Leuven

发明人： Jones, Benjamin ,  Fiorini, Paolo ,  Tanaka, Hiroyuki

IPC分类号： B01L7/00 ,  B01L3/00 ,  B81C1/00 ,  B81B3/00 ,  B81B7/00

CPC分类号： B01L3/502707 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/1805 ,  B01L2300/1883 ,  B81B3/0081 ,  B81B7/0087 ,  B81B2201/051

摘要： The present invention provides a micro-fluidic device (104), comprising: - a semiconductor substrate; - at least one micro-reactor (105) in said semiconductor substrate; - one or more micro-fluidic channels (101) in said semiconductor substrate, connected to said at least one micro-reactor (105); - a cover layer (106) bonded to the semiconductor substrate (900) for sealing the one or more micro-fluidic channels (101); and - at least one through-substrate trench (100) surrounding said at least one micro-reactor (105) and the one or more micro-fluidic channels (101).

公开(公告)号：EP2896457B1

公开(公告)日：2017-08-23

申请号：EP14151290.5

申请日：2014-01-15

申请人： IMEC VZW

发明人： Jones, Benjamin ,  Fiorini, Paolo

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2300/0838 ,  B01L2300/12 ,  B01L2400/0406 ,  B01L2400/0688 ,  Y10T137/87917

公开(公告)号：EP2896457A1

公开(公告)日：2015-07-22

申请号：EP14151290.5

申请日：2014-01-15

申请人： IMEC VZW

发明人： Jones, Benjamin ,  Fiorini, Paolo

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2300/0838 ,  B01L2300/12 ,  B01L2400/0406 ,  B01L2400/0688 ,  Y10T137/87917

摘要： The embodiments of the present invention disclose a micro-fluidic device comprising a substrate, a cavity in the substrate and a plurality of micro-pillar columns located inside the cavity. The micro-pillars columns are configured to create a capillary action when a fluid sample is provided in the cavity. A micro-fluidic channel is present between two walls of any two adjacent micro-pillars in a same micro-pillar column. Each of the two walls comprises a sharp corner along the direction of a propagation path of the fluid sample in the micro-fluidic channel thereby forming a capillary stop valve. A notch provided in a sidewall of the cavity acts as a capillary stop valve.

摘要翻译： 本发明的实施例公开了一种微流体装置，其包括衬底，衬底中的腔和位于空腔内的多个微柱。 微柱柱配置成当在空腔中提供流体样品时产生毛细管作用。 微流体通道存在于相同微柱柱中任意两个相邻微柱的两个壁之间。 两个壁中的每一个包括沿微流体通道中的流体样品的传播路径的方向的尖角，从而形成毛细管截止阀。 设置在腔的侧壁中的凹口用作毛细管截止阀。