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1.发明公开Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same 审中-公开COENZYM-VERKNÜPFTEGLUCOSE-DEHYDROGENASE UNDDAFÜRCODIERENDES POLYNUCLEOTID
公开(公告)号：EP2365074A1
公开(公告)日：2011-09-14
申请号：EP11156661.8
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体的存在下催化葡萄糖的氧化并具有麦芽糖的活性为5％以下; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集与FAD连接以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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公开(公告)号：EP2365073A1
公开(公告)日：2011-09-14
申请号：EP11156659.2
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：EP2380980B1
公开(公告)日：2014-11-05
申请号：EP11156649.3
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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4.发明公开Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same 审中-公开Coenzym-verknüpfte葡萄糖脱氢酶和dafürcodierendes Polynucleotid
公开(公告)号：EP2380981A1
公开(公告)日：2011-10-26
申请号：EP11156664.2
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体的存在下催化葡萄糖的氧化并具有麦芽糖的活性为5％以下; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集与FAD连接以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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公开(公告)号：EP2380980A1
公开(公告)日：2011-10-26
申请号：EP11156649.3
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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6.发明公开Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same 审中-公开辅酶连接的葡萄糖脱氢酶和编码它的多核苷酸
公开(公告)号：EP2368987A1
公开(公告)日：2011-09-28
申请号：EP11156657.6
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大量生产对葡萄糖具有优异的底物识别能力同时对麦芽糖提供低作用的辅酶连接的葡萄糖脱氢酶的成员。 本发明涉及编码可溶性辅酶连接的葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％或更低的麦芽糖活性; 由该多核苷酸的核苷酸序列编码的多肽; 携带该多核苷酸的重组载体; 使用重组载体产生的转化细胞; 产生多肽的方法，包括培养转化的细胞并从培养产物中收集与FAD连接以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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