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1.发明公开Process for producing dietary sterol fatty acid esters 审中-公开在Nahrungsmitteln的Verfahren zur Herstellung vonSterolfettsäureestern
公开(公告)号：EP1209239A2
公开(公告)日：2002-05-29
申请号：EP01250411.4
申请日：2001-11-22
发明人： Norinobu, Seiji , Seo, Naoko , Sato, Fumi , Kaneko, Shoji , Mankura, Mitsumasa
摘要： A process for enzymatically producing, from a vegetable oil deodorizer distillate, a dietary sterol fatty acid ester superior in flavor qualities (e.g., color, odor and taste) and safety and containing no or little trans fatty acids, wherein the conditions for treatment of the starting material, synthetic reaction and subsequent purification are controlled so that the sterol fatty acid ester becomes applicable as a daily food material, a health food material or a pharmaceutical material. In the process, fatty acid esters, e.g., triacylglycerol, in the vegetable oil deodorizer distillate are previously degraded by hydrolysis with a chemical catalyst, fatty acids produced in the hydrolysis are removed by molecular distillation to give a sterol-containing fraction. The sterol-containing fraction is added with any fat and oil primarily comprising triacylglycerol. The mixture is used as the starting material, and the synthetic reaction is performed under strictly controlled conditions using a lipolytic enzyme.
The resulting product undergoes several steps of purification process to provide qualities suitable for a food material.摘要翻译： 一种从植物油除臭馏分中提取出具有优良风味品质（如颜色，气味和味道）和安全性且不含或少量反式脂肪酸的膳食甾醇脂肪酸酯的方法，其中处理 控制原料，合成反应和随后的纯化，使得固醇脂肪酸酯可以用作日常食品材料，保健食品材料或药物材料。 在此过程中，植物油除臭馏分中的脂肪酸酯，例如三酰基甘油，预先通过用化学催化剂水解而降解，通过分子蒸馏除去在水解中产生的脂肪酸，得到含固醇馏分。 加入含甾醇的级分中主要包含三酰基甘油的任何脂肪和油。 将混合物用作起始原料，并使用脂肪分解酶在严格控制的条件下进行合成反应。 所得产物经过几个步骤的纯化过程以提供适合食品材料的质量。
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公开(公告)号：EP3438278A1
公开(公告)日：2019-02-06
申请号：EP17775006.4
申请日：2017-03-28
发明人： NAKATSUKA, Tomoko , AOKI, Hideyuki , KIDA, Mikiko , YAMADA, Kenta , KANEKO, Shoji , KUGIMIYA, Akimitsu
IPC分类号： C12Q1/25 , C12Q1/68 , G01N27/416 , G01N33/68
摘要： [Problem] To provide: a method for selectively and easily quantifying an amino acid to be measured (L-form or D-form amino acid) with high sensitivity using aminoacyl-tRNA synthetase (AARS); and an amino acid quantification kit. [Solution] The present invention pertains to: a method for quantifying an amino acid (L-form or D-form amino acid) in a sample by means of AARS, said method being characterized in that AARS and an amino acid are released from an aminoacyl-AMP AARS complex once formed and are used once again for forming an aminoacyl-AMP AARS complex so as to ultimately produce a reaction product such as pyrophoric acid to be measured in an amount that is greater in terms of the number of moles than the amino acid included in the sample; and an amino acid quantification kit for implementing said method.
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3.发明授权METHOD FOR DETERMINATION OF 1,5-ANHYDROGLUCITOL, AND REAGENT COMPOSITION FOR DETERMINATION OF 1,5-ANHYDROGLUCITOL 有权用于确定1.5脱水和试剂组合物为1,5-脱水葡萄糖醇的测定
公开(公告)号：EP2039765B1
公开(公告)日：2011-10-05
申请号：EP07767432.3
申请日：2007-06-22
发明人： ISHIMARU, Emi , SANADA, Hirokazu , OMURA, Hironori , YOSHIOKA, Hideki , TSUKAMOTO, Shuhei , MASUDA, Minoru
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/66 , G01N33/6893 , G01N2800/042
摘要： A method for the determination of 1,5-anhydroglucitol, characterized by using any one of the following proteins (a) to (c): (a) a protein which comprises an amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence having the deletion, substitution and/or addition of one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO:2 and which has a sorbose dehydrogenase activity; and (c) a protein which comprises an amino acid sequence having at least 60% sequence homology to the amino acid sequence depicted in SEQ ID NO:2 and which has a sorbose dehydrogenase activity.
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4.发明公开METHOD FOR DETERMINATION OF 1,5-ANHYDROGLUCITOL, AND REAGENT COMPOSITION FOR DETERMINATION OF 1,5-ANHYDROGLUCITOL 有权用于确定1.5脱水和试剂组合物为1,5-脱水葡萄糖醇的测定
公开(公告)号：EP2039765A1
公开(公告)日：2009-03-25
申请号：EP07767432.3
申请日：2007-06-22
发明人： ISHIMARU, Emi , SANADA, Hirokazu , OMURA, Hironori , YOSHIOKA, Hideki , TSUKAMOTO, Shuhei , MASUDA, Minoru
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/66 , G01N33/6893 , G01N2800/042
摘要： The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.
摘要翻译： 本发明的目的是提供一种采矿确定性1.5脱水的方法，包括使用（A），它besteht SEQ ID NO：2的氨基酸序列组成的蛋白质：2; （B）的氨基酸哪besteht具有序列缺失，取代和/或添加的SEQ ID NO的氨基酸序列的一个或多个氨基酸残基组成的蛋白质：2，且具有山梨糖脱氢酶活性; 或（c）其中besteht具有至少60％与SEQ ID NO的氨基酸序列的同源性的氨基酸序列组成的蛋白质：2，且具有山梨糖脱氢酶活性。
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5.发明公开COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME 有权COENZYM-VERKNÜPFTEGLUCOSE-DEHYDROGENASE UNDDAFÜRCODIERENDES POLYNUKLEOTID
公开(公告)号：EP1862543A1
公开(公告)日：2007-12-05
申请号：EP06730146.5
申请日：2006-03-27
发明人： OMURA, Hironori , SANADA, Hirokazu , YADA, Takako , ATSUMI, Ayaka , MORITA, Tetsunari , ISHIMARU, Emi
IPC分类号： C12N15/09 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12Q1/32 , C12R1/645 , C12R1/66 , C12R1/69
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体的存在下催化葡萄糖的氧化并具有麦芽糖的活性为5％以下; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集与FAD连接以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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公开(公告)号：EP2380980B1
公开(公告)日：2014-11-05
申请号：EP11156649.3
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：EP2426200A1
公开(公告)日：2012-03-07
申请号：EP10769837.5
申请日：2010-04-30
发明人： TAKENAKA Ryo , HONDA Michinari , OMURA Hironori
IPC分类号： C12N15/09 , C07K14/38 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12P21/02 , C12Q1/00 , G01N27/327 , H01M8/16
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.
The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.摘要翻译： 要解决的问题是提供与酶具有高亲和力的电子介体和融合体，使用细胞外分泌型细胞色素和酶的测量方法，电极和传感器。 本发明涉及包含细胞外分泌型细胞色素的葡萄糖氧化还原酶用电子传递体，电子传递体与葡萄糖氧化还原酶融合的融合体，包含电子传递体或融合体的葡萄糖测定用组合物，编码新的细胞外 分泌型细胞色素，以及使用细胞外分泌型细胞色素和酶的测量方法，电极和传感器。
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8.发明公开Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same 审中-公开Coenzym-verknüpfte葡萄糖脱氢酶和dafürcodierendes Polynucleotid
公开(公告)号：EP2380981A1
公开(公告)日：2011-10-26
申请号：EP11156664.2
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体的存在下催化葡萄糖的氧化并具有麦芽糖的活性为5％以下; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集与FAD连接以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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公开(公告)号：EP2380980A1
公开(公告)日：2011-10-26
申请号：EP11156649.3
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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10.发明公开Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same 审中-公开辅酶连接的葡萄糖脱氢酶和编码它的多核苷酸
公开(公告)号：EP2368987A1
公开(公告)日：2011-09-28
申请号：EP11156657.6
申请日：2006-03-27
发明人： Omura, Hironori , Sanada, Hirokazu , Yada, Takako , Atsumi, Ayaka , Morita, Tetsunari , Ishimaru, Emi
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大量生产对葡萄糖具有优异的底物识别能力同时对麦芽糖提供低作用的辅酶连接的葡萄糖脱氢酶的成员。 本发明涉及编码可溶性辅酶连接的葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％或更低的麦芽糖活性; 由该多核苷酸的核苷酸序列编码的多肽; 携带该多核苷酸的重组载体; 使用重组载体产生的转化细胞; 产生多肽的方法，包括培养转化的细胞并从培养产物中收集与FAD连接以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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