公开(公告)号：EP4306532A3

公开(公告)日：2024-04-10

申请号：EP23203880.2

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C07H1/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na 2 S 2 O 4 . Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：EP3794012A1

公开(公告)日：2021-03-24

申请号：EP19727252.9

申请日：2019-05-14

申请人： Illumina Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C07H21/04 ,  C12Q1/6806 ,  C12Q1/6874

公开(公告)号：EP4306532A2

公开(公告)日：2024-01-17

申请号：EP23203880.2

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C07H19/20

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na 2 S 2 O 4 . Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.