公开(公告)号：EP4306532A3

公开(公告)日：2024-04-10

申请号：EP23203880.2

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C07H1/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na 2 S 2 O 4 . Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：EP3837384A1

公开(公告)日：2021-06-23

申请号：EP20708085.4

申请日：2020-03-02

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： ROMANOV, Nikolai ,  HONG, Stanley S. ,  MCCAULEY, Patrick ,  LIU, Xiaohai ,  CONDELLO, Danilo ,  SIU, Merek

IPC分类号： C12Q1/6869

公开(公告)号：EP3794012A1

公开(公告)日：2021-03-24

申请号：EP19727252.9

申请日：2019-05-14

申请人： Illumina Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C07H21/04 ,  C12Q1/6806 ,  C12Q1/6874

公开(公告)号：EP4306532A2

公开(公告)日：2024-01-17

申请号：EP23203880.2

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C07H19/20

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na 2 S 2 O 4 . Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：EP3592865A1

公开(公告)日：2020-01-15

申请号：EP18712067.0

申请日：2018-03-06

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： LANGLOIS, Robert ,  VIECELI, John ,  LIU, Xiaohai

IPC分类号： C12Q1/6874 ,  B01J19/00 ,  G01N21/64

公开(公告)号：EP4168421A1

公开(公告)日：2023-04-26

申请号：EP21737355.4

申请日：2021-06-21

申请人： Illumina Cambridge Limited

发明人： FRANCAIS, Antoine ,  CRESSINA, Elena ,  MARIANI, Angelica ,  CULLEY, Adam ,  KOETJE, Anno ,  LIU, Xiaohai

IPC分类号： C07H1/02 ,  C07H19/10 ,  C07H19/20 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6874

公开(公告)号：EP2964624B1

公开(公告)日：2017-01-04

申请号：EP13709182.3

申请日：2013-03-08

申请人： Illumina Cambridge Limited

发明人： ROMANOV, Nikolai, Nikolaevich ,  LIU, Xiaohai

IPC分类号： C07D311/82 ,  C07H21/00 ,  C09B11/24 ,  G01N33/58

CPC分类号： C07D311/82 ,  C07H19/10 ,  C07H21/00 ,  C09B11/24 ,  G01N33/582

公开(公告)号：EP2970356A1

公开(公告)日：2016-01-20

申请号：EP13711333.8

申请日：2013-03-15

申请人： Illumina Cambridge Limited

发明人： LIU, Xiaohai ,  WU, Xiaolin ,  SMITH, Geoffrey Paul

IPC分类号： C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/173 ,  C07H19/20

CPC分类号： C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/173 ,  C07H19/20 ,  C12Q1/6869 ,  C12Q1/6876 ,  Y02P20/55

摘要： Some embodiments described herein relate to modified nucleotide and nucleoside molecules with novel 3′-hydroxy protecting groups. Also provided herein are methods to prepare such modified nucleotide and nucleoside molecules and sequencing by synthesis processes using such modified nucleotide and nucleoside molecules.

摘要翻译： 本文所述的一些实施方案涉及具有新的3'-羟基保护基团的修饰的核苷酸和核苷分子。 本文还提供了通过使用这种修饰的核苷酸和核苷分子的合成方法制备这种修饰的核苷酸和核苷分子和测序的方法。

公开(公告)号：EP4267765A1

公开(公告)日：2023-11-01

申请号：EP21844256.4

申请日：2021-12-21

申请人： Illumina Cambridge Limited

发明人： WU, Xiaolin ,  ANASTASI, Carole ,  EVANS, Geraint ,  LIU, Xiaohai

IPC分类号： C12Q1/6869

公开(公告)号：EP3848380A1

公开(公告)日：2021-07-14

申请号：EP21156755.7

申请日：2003-08-22

申请人： Illumina Cambridge Limited

发明人： MILTON, John ,  RUEDIGER, Silke ,  LIU, Xiaohai

IPC分类号： C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/68 ,  G01N33/53

摘要： The invention provides a nucleotide or nucleoside having a 3'-azidomethyl group and a base attached to a detectable label via a linker.